Immobilized lipase from Hypocrea pseudokoningii on hydrophobic and ionic supports: Determination of thermal and organic solvent stabilities for applications in the oleochemical industry

Pereira, Maria José Bistafa; Facchini, Fernanda Dell Antonio; Filó, Luiz Estevam Cavenage; Polizeli, Aline Moraes; Vici, Ana Claudia; Jorge, João Atı́lio; Fernández‐Lorente, Gloria; Pessela, Benevides C.; Guisán, José M.; Polizeli, Maria de Lourdes Teixeira de Moraes

doi:10.1016/j.procbio.2014.12.027

Cited by 27 publications

(29 citation statements)

References 22 publications

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“…A reference solution with soluble enzyme was submitted to the same treatment to detect any possible effect of Triton X-100 on the enzyme activity [23].…”

Section: Desorption Of Lipase From the Octyl-sepharose Supportmentioning

confidence: 99%

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Stabilization of the lipase of Hypocrea pseudokoningii by multipoint covalent immobilization after chemical modification and application of the biocatalyst in oil hydrolysis

Pereira

Facchini

Polizeli

et al. 2015

Journal of Molecular Catalysis B: Enzymatic

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“…A reference solution with soluble enzyme was submitted to the same treatment to detect any possible effect of Triton X-100 on the enzyme activity [23].…”

Section: Desorption Of Lipase From the Octyl-sepharose Supportmentioning

confidence: 99%

“…Due to changes in the code of nomenclature, the genus Trichoderma has been proposed for conservation over its teleomorph Hypocrea [21]. The lipase production for Hypocrea pseudokoningii was previously identified and characterized in our laboratory and the lipase was immobilized on ionic and hydrophobic supports [22,23].…”

Section: Introductionmentioning

confidence: 99%

Stabilization of the lipase of Hypocrea pseudokoningii by multipoint covalent immobilization after chemical modification and application of the biocatalyst in oil hydrolysis

Pereira

Facchini

Polizeli

et al. 2015

Journal of Molecular Catalysis B: Enzymatic

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“…Vários polímeros são utilizados como suporte para imobilização de enzimas, tais como agarose, sefarose, decaoctil, nanotubos de carbono (ASURI et al, 2006;PEREIRA et al, 2015). Alguns estudos indicam o uso de materiais lignocelulósicos e celulósicos com o mesmo intuito, dando também alternativa ao reaproveitamento dos resíduos agroindustriais (BRÍGIDA, 2010;KIM et al, 2015;KOSAKA et al, 2007;SUN et al, 2015).…”

Section: Lista De Figurasunclassified

“…Esses biocatalisadores demonstram melhor desempenho em meios orgânicos e anidros, minimizando assim a dessorção das enzimas imobilizadas (FERNANDEZ-LAFUENTE et al, 1998). Diversos materiais são utilizados como suporte para imobilizar enzimas via adsorção, como polímeros hidrofóbicos e iônicos (PEREIRA et al, 2015), matrizes celulósicas (BRÍGIDA, 2010;KIM et al, 2015;KOSAKA et al, 2007) (FERNANDEZ-LAFUENTE et al, 1998;GARCIA-GALAN et al, 2014;PEREIRA et al, 2015). Essas variações podem ser diferentes para uma mesma enzima, dependendo do suporte utilizado e do protocolo de imobilização (CABRERA et al, 2008;HIRATA et al, 2016a;PALOMO et al, 2002).…”

Section: Lista De Figurasunclassified

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Emprego de derivados celulósicos esterificados na imobilização da fosfolipase Lecitase® Ultra

Rodrigues¹

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AGRADECIMENTOSAo Criador, pela oportunidade de viver, pelo sopro de vida que me impulsiona e por permitir reconhecê-lo como o sentido de tudo.Aos meus pais, Wilson e Rejane, pela instrução que me deram, ensinamentos, apoio, respeito, amor e por sempre acreditarem em mim. Reverencio tudo que fizeram por mim e me fizeram ser o que sou.Aos meus avós, Jeovah e Laudelina, por conseguirem me ensinar apenas com sua existência.À minha irmã, Meni Loren, e ao meu cunhado, Neilton, pelo carinho e respeito sempre dispensados.Aos meus pequenos, Murilo, Isabele, Sophia, Mariana e Maurício, por me ensinarem a beleza, a cor e a pureza da vida.À toda minha família. Vocês são o abraço que Deus me dá. showed that the use of TAC as support for adsorption of LU proved promising, due to the high capacity of enzymatic retention and maintenance of the catalytic activity of the enzyme after immobilization. Aos meus amigos

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Potential application of Thermomyces lanuginosus lipase (TLL) immobilized on nonporous polystyrene particles

Dantas

Valério

Ninow

et al. 2018

Env Prog and Sustain Energy

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The crescent demand for biocatalyst application, associated with the production costs and the need to reduce the dependence for the fossil fuels combined with waste recovery, which could be used as an alternative to resources, increased the interests for the enzyme immobilization. In this work, low‐cost nonporous polystyrene (PS) particles were studied as a support to Thermomyces Lanuginosus lipase (NS‐40116) immobilization. The results showed that immobilized lipase on PS matrix showed better thermal and pH stability when compared to soluble NS‐40116 lipase, with an increase of 84% in the hydrolytic activity at pH 12. Kinetic constants showed that immobilization process increased enzyme specificity by the substrate. In addition, immobilized lipase on PS matrix was effectively applied in the production of free fatty acids from commercial soybean oil demonstrating operational stability after five reuse cycles as well as potential application in the transesterification and esterification reactions by using abdominal chicken fat as substrate. © 2018 American Institute of Chemical Engineers Environ Prog, 38: 608–613, 2019

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Immobilized lipase from Hypocrea pseudokoningii on hydrophobic and ionic supports: Determination of thermal and organic solvent stabilities for applications in the oleochemical industry

Cited by 27 publications

References 22 publications

Stabilization of the lipase of Hypocrea pseudokoningii by multipoint covalent immobilization after chemical modification and application of the biocatalyst in oil hydrolysis

Stabilization of the lipase of Hypocrea pseudokoningii by multipoint covalent immobilization after chemical modification and application of the biocatalyst in oil hydrolysis

Emprego de derivados celulósicos esterificados na imobilização da fosfolipase Lecitase® Ultra

Potential application of Thermomyces lanuginosus lipase (TLL) immobilized on nonporous polystyrene particles

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