Immobilized Forms of the Ophiostoma piceae Lipase for Green Synthesis of Biodiesel. Comparison with Eversa Transform 2.0 and Cal A

Abstract

In this work, we analyzed the suitability of a versatile recombinant lipase, secreted by Ophiostoma piceae (OPEr) and produced in Pichia pastoris, as a catalyst of the synthesis of biodiesel. The enzyme was immobilized by five covalent procedures and by hydrophobicity on functionalized nanoparticles of magnetite or of a novel Zn/Mn oxide named G1. Then, they were tested for green production of biodiesel by solventless enzymatic transesterification of discarded cooking oil and methanol (1:4) at 25 °C. The results were compared with those shown by free OPEr and the commercial lipases Eversa® and Cal A®. Several preparations with immobilized OPEr produced high synthesis yields (>90% transesterification), comparable to those obtained with Eversa®, the commercial enzyme designed for this application. Three of the biocatalysts maintained their catalytic efficiency for nine cycles. The process catalyzed by AMNP-CH-OPEr was scaled from 500 µL to 25 mL (50 times), improving its efficiency.

Decreasing acid value of fatty acid ethyl ester products using complex enzymes

“…Furthermore, water in the reaction system may inhibit the positive progression of the lipase-catalyzed reaction and result in the high acid value of the product ( Hájek et al, 2012 ; Nguyen et al, 2018 ). During the reaction, the surface of the lipase Eversa ® Transform 2.0 requires a layer of essential water to maintain a certain active conformation for its catalytic activity ( Molina-Gutiérrez et al, 2021 ). Therefore, the dehydration capacity of the water removal agent should be appropriate, so that not only the acid value of the FAEE product is desired, but also an essential layer of water is not taken away from the surface of the enzyme.…”

Molecular identification and lipolytic potential of filamentous fungi isolated from residual cooking oil

“…The recognition of filamentous fungi with hydrolytic enzymatic activity suggests that they are preferred sources for the industry, due to superior metabolic versatility ( Su et al 2012 ). Fungal enzymes are used in biofuel production processes, bioremediation of organic contaminants, for detergent production, antibiotic production, bleaching of textile fabrics, juice clarification, removal of leather aromas and paper production ( El-Sawah et al 2021 , Füting et al 2021 , Hyde et al 2019 , Kirk et al 2002 , Meyer et al 2020 , Molina-Gutiérrez et al 2021 , Niyonzima 2020 , Singh et al 2021 , Sudeep et al 2020 , Syedd-León et al 2022 ).…”

“…From a social and economic perspective, these microorganisms play an important role in the pharmaceutical, agricultural, food, detergent, leather, paper industries and biofuel ( El-Sawah et al 2021 , Hyde et al 2019 , KC et al 2020 , Meyer 2021 , Molina-Gutiérrez et al 2021 , Singh et al 2021 ), for the generation of hydrolytic enzymes, which can be used in novel biotechnological processes ( Ramos Sanchez 2015 , Syedd-León et al 2022 ).…”

Indigenous Materials as Catalyst Supports for Renewable Diesel Production in Malaysia

“…In that study, the outstanding catalytic stability of the mentioned catalyst was discovered even after the fifth reused cycle and is capable of achieving a fatty acid methyl ester (FAME) yield of around 93%. Other than heterogeneous catalysts, biocatalysts such as immobilized lipase from Bacillus mycoides and Ophiostoma piceae strains, as well as fermented macaúba cake are used to assist in enzymatic transesterification [18][19][20]. Biocatalysts are studied as they are eco-friendly, generate minimal waste while consuming less energy, and function well under mild process conditions [20].…”