Immobilized enzyme electrode for the determination of salicylate in blood serum

A Polypyrrole-Bienzyme Electrode (Salicylate Hydroxylase-Polyphenol Oxidase) for the Interference-Free Determination of Salicylate

“…As previously reported by Rahni et al [24], an optimum temperature range between 25 and 30 C was determined, the biosensor sensitivity decreasing for higher temperatures. Consequently, the amperometric measurements were carried out at 30 C. Since NADH is not stable at pH values lower than 5.5, the pH effect on the activity of poly1-SH electrode was examined in the pH range from 6 to 8.…”

Study of NADH Stability Using Ultraviolet–Visible Spectrophotometric Analysis and Factorial Design

“…Enzymes are being used in a great number of analytical procedures in the search for greater selectivity in complex sample analysis in clinical (1,2), environmental (3), food (4), and pharmaceutical assays (5) and biosensors employ them as biologically active components (6 -12). Many enzymes depend on the nicotinamide adenine dinucleotide (NADH) cofactor to act with the substrate, thus extensive studies about NADH monitoring are described in the literature (13)(14)(15).…”

The properties and applications of amperometric gas sensors

“…In biochemistry, amperometric serisors (although not gas sensors) have been applied t o the determination of proteins, root death, total iron and the concentration of NO,. It has been rcported that the glucose oxidase enzyme can be immobilized at the sureace of the oxygen electrode, and oxygen deficiency at the working electrode due to the enzymatic oxidation has been used for in-vivo and ex-vivo monitoringofglucose [51][52][53][54][55][56][57][58][59][60], lactate [61][62][63],xanthine [64],ascorbic acid [65], microbial grouch [66,67], dopamine [68], and salicylate [ 69,701. The utilization of amperometric sensors ol all kinds for the determination of environmental pollutants has been reviewed recently by Smart [71] and particularly by Janata 1721. hmperometric sensor is used in industry hygiene and safety to determine the quality of the workplace environment.…”