Immobilized cells: biocatalysts and processes

Андрюшина, В. А.; Andryushina, V. A.; Tolstova, Tatiana; Balabanova, Tat'yana; Беклемишев, Анатолий; Беклемишев, А. Б.; Varfolomeev, S. D.; Варфоломеев, С. Д.; Водякова, Марина; Vodyakova, M. A.; Демаков, Виталий; Demakov, Vitaliy; Дитченко, Татьяна; Ditchenko, Tat'yana; Джавахия, Вахтанг; Dzhavahiya, Vahtang; Дроздова, М. Г.; Drozdova, Mariya; Ефременко, Елена; Завьялова, Наталья; Zav'yalova, Natal'ya; Ившина, И. Б.; Ismailov, A. D.; Karpova, N. V.; Karpova, N. V.; Коваленко, Г. А.; Krivoruchko, Anastasiia V.; Krivoruchko, Anastasiya V.; Куюкина, М. С.; Kuyukina, Mariya; Lozinskii, V. I.; Lozinskiy, Vladimir; Лягин, Илья; Lyagin, Ilya; Максимова, Юлия; Maksimova, Yuliya; Мамедова, Фахрия; Mamedova, F.; Марквичева, Елена; Maslova, Olga; Махлис, Т.А.; Mahlis, Tat'yana; Молчан, О. В.; Perminova, L. V.; Плеханова, Юлия; Plehanova, Yuliya; Решетилов, А Н; Reshetilov, Anatoliy; Senko, Olga; Stepanov, Nikolay; Stytsenko, T. S.; Stycenko, Tat'yana; Филиппова, С. Е.; Холстов, Александр; Holstov, Aleksandr; Юрин, Владимир; Yurin, Vladimir; Ядерец, Вера; Yaderec, Vera

doi:10.29039/02004-3

Cited by 12 publications

(9 citation statements)

References 37 publications

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“…The mechanism of the influence of various toxic compounds on the bioluminescence of bacterial Photobacterium phosphoreum cells depends on the chemical nature of these compounds, but the sensitivity of the toxins’ determination essentially depends on the form of the cells used for the analysis (suspended or immobilized) [32]. Therefore, the purpose of this work was to assess the feasibility of using cells of bioluminescent bacteria P. phosphoreum immobilized in PVA cryogel for detecting several mycotoxins under discrete and flow-through conditions.…”

Section: Introductionmentioning

confidence: 99%

Immobilized Luminescent Bacteria for the Detection of Mycotoxins under Discrete and Flow-Through Conditions

et al. 2019

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A biosensitive element in the form of bacterial Photobacterium phosphoreum cells immobilized in poly(vinyl alcohol) cryogel was tested for the determination of different mycotoxins under discrete and flow-through analysis conditions. The immobilized bioluminescent cells made it possible to quantify the presence of Ochratoxin A, Sterigmatocystin, Zearalenone, and Deoxynivalenon in aqueous media in a wide range of their concentrations (0.017–56 mg/L, 0.010–33 mg/L, 0.009–14 mg/L, and 0.026–177 mg/L, respectively) via measuring the quenching of cell luminescence. The flow conditions allowed the analysis sensitivity to be improved by an order of magnitude in terms of detected concentrations. Using the immobilized luminescent bacterial cells, we have shown the possibility of evaluating the efficiency of the mycotoxins’ hydrolysis under the action of enzymes. In this way, a 94 ± 4.5% efficiency of Zearalenone hydrolysis with hexahistidine-containing organophosphorus hydrolase for 1h-long treatment of the mycotoxin solution (100 mg/L) was shown.

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Section: Introductionmentioning

confidence: 99%

Immobilized Luminescent Bacteria for the Detection of Mycotoxins under Discrete and Flow-Through Conditions

et al. 2019

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“…It is known that an important characteristic of immobilized cells that significantly distinguishes them from suspension cells is their high operational stability [39]. In this regard, in order to suppress the metabolic activity of immobilized cells, it is likely that slightly higher concentrations of HC will be required than for suspension cells or re-introduction of HC derivatives in the case of their practical application in landfills to suppress methanogenesis.…”

Section: Discussionmentioning

confidence: 99%

Suppression of Methane Generation during Methanogenesis by Chemically Modified Humic Compounds

et al. 2020

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The introduction of various concentrations of chemically modified humic compounds (HC) with different redox characteristics into the media with free and immobilized anaerobic consortia accumulating landfill gases was studied as approach to their functioning management. For this purpose, quinone (hydroquinone, naphthoquinone or methylhydroquinone) derivatives of HC were synthesized, which made it possible to vary the redox and antioxidant properties of HC as terminal electron acceptors in methanogenic systems. The highest acceptor properties were obtained with potassium humate modified by naphthoquinone. To control possible negative effect of HC on the cells of natural methanogenic consortia, different bioluminescent analytical methods were used. The addition of HC derivatives, enriched with quinonones, to nutrient media at concentrations above 1 g/L decreased the energetic status of cells and the efficiency of the methanogenesis. For the first time, the significant decrease in accumulation of biogas was reached as effect of synthetic HC derivatives, whereas both notable change of biogas composition towards increase in the CO2 content and decrease in CH4 were revealed. Thus, modification with quinones makes it possible to obtain low-potential HC derivatives with strongly pronounced acceptor properties, promising for inhibition of biogas synthesis by methanogenic communities.

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“…Cells of all bacterial cultures and samples of ASs were immobilized into the PVA cryogel according to previously developed techniques [14,28,29,30]. To realize that, the biomass precipitate was thoroughly mixed with a 10% ( w/v ) aqueous PVA solution to obtain a 10% ( w/w ) concentration of bacterial cells, and a 30% concentration ( w/w ) of anaerobic sludge.…”

Section: Methodsmentioning

confidence: 99%

Prospective Approach to the Anaerobic Bioconversion of Benzo- and Dibenzothiophene Sulfones to Sulfide

et al. 2019

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Sulfur recovery from organic molecules such as toxic sulfones is an actual problem, and its solution through the use of environmentally friendly and nature-like processes looks attractive for research and application. For the first time, the possible bioconversion of organic sulfones (benzo-and dibenzothiophene sulfones) to inorganic sulfide under anaerobic conditions with simultaneous biogas production from glucose within a methanogenesis process is demonstrated. Biogas with a methane content of 50.7%–82.1% was obtained without H2S impurities. Methanogenesis with 99.7%–100% efficiency and 97.8%–100% conversion of benzo- and dibenzothiophene sulfones (up to 0.45 mM) to inorganic sulfide were obtained in eight days by using a combination of various anaerobic biocatalysts immobilized in a poly(vinyl alcohol) cryogel. Pure cell cultures of sulfate-reducing bacteria and/or H2-producing bacteria were tested as additives to the methanogenic activated sludge. The immobilized activated sludge “enhanced” by bacterial additives appeared to retain its properties and be usable multiple times for the conversion of sulfones under batch conditions.

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Immobilized cells: biocatalysts and processes

Cited by 12 publications

References 37 publications

Immobilized Luminescent Bacteria for the Detection of Mycotoxins under Discrete and Flow-Through Conditions

Immobilized Luminescent Bacteria for the Detection of Mycotoxins under Discrete and Flow-Through Conditions

Suppression of Methane Generation during Methanogenesis by Chemically Modified Humic Compounds

Prospective Approach to the Anaerobic Bioconversion of Benzo- and Dibenzothiophene Sulfones to Sulfide

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