Immobilized Cell Physiology Imaging and Stabilization of Enzyme Cascade Reaction Using Recombinant Cells Escherichia coli Entrapped in Polyelectrolyte Complex Beads by Jet Break-Up Encapsulator
Abstract:A novel, high performance, and scalable immobilization protocol using a laminar jet break-up technique was developed for the production of polyelectrolyte complex beads with entrapped viable Escherichia coli cells expressing an enzyme cascade of alcohol dehydrogenase, enoate reductase, and cyclohexanone monooxygenase. A significant improvement of operational stability was achieved by cell immobilization, which was manifested as an almost two-fold higher summative product yield of 63% after five cascade reactio… Show more
“…In the final research article, the group of Prof. Mihovilovic [7] tackle biocatalysis with the combination of enzymes in cascade reactions. Several whole-cell enzyme cascade systems using the effect of shifting the reaction equilibrium using an irreversible reaction catalysed by BVMOs, have been previously reported to maximise the yield of the desired product.…”
Flavin-containing monooxygenase are a large family of enzymes involved in an array of different reactions by activating molecular oxygen and transferring one atom of oxygen to their substrates [...]
“…In the final research article, the group of Prof. Mihovilovic [7] tackle biocatalysis with the combination of enzymes in cascade reactions. Several whole-cell enzyme cascade systems using the effect of shifting the reaction equilibrium using an irreversible reaction catalysed by BVMOs, have been previously reported to maximise the yield of the desired product.…”
Flavin-containing monooxygenase are a large family of enzymes involved in an array of different reactions by activating molecular oxygen and transferring one atom of oxygen to their substrates [...]
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