Immobilized carbonic anhydrase on mesoporous cruciate flower-like metal organic framework for promoting CO2 sequestration

Fast Immobilization of Human Carbonic Anhydrase II on Ni-Based Metal-Organic Framework Nanorods with High Catalytic Performance

“…Carbonic anhydrase (CA, E.C. 4.2.1.1), a zinc metalloenzyme, can efficiently catalyze the reversible hydration of CO 2 to bicarbonate ion [1,2]. In nature, chemical fixation of CO 2 usually requires high temperatures, high pressure and long reaction times, while carbonic anhydrase can fix carbon dioxide and have a high reaction rate under low pressure conditions [3].…”

Novel bovine carbonic anhydrase encapsulated in a metal–organic framework: a new platform for biomimetic sequestration of CO2

“…The TGA curve of ZIF-8 and BCA-ZIF-8 show an initial weight loss related to the removal of guest water molecules and residual imidazole in the pores within the range of 20-200 C, followed by the next weight loss in the range of 200-300 C attributed to the loss of the BCA enzyme. 39 By comparison of step two in the TGA curves of BCA-ZIF-8 and ZIF-8, it can be seen that the weight loss of BCA-ZIF-8 is larger than that of pure ZIF-8 which proves the claim about the presence of BCA in the framework.…”

“…Additionally, the existence of the band at 1648 cm À1 corresponding to double bonds and C]O stretching modes of the enzyme veried the presence of the protein in the composites. 39 Moreover, the free BCA spectrum exhibited stretching bands at 1500-1550 cm À1 corresponding to amide II bonds and NH stretching vibration which were visible in the biocatalyst spectrum, conrming the presence of the CA within the ZIF-8 (Fig. 2c).…”

“…38 Cui et al successfully designed a combined immobilization system of CA and ZIF-8 by the physical adsorption of CA onto the external surface of ZIF-8. 39 The synthesized composite was utilized as a robust biocatalyst for the sequestration of CO 2 , but this immobilized system suffered from problems of weak interactions such as lower enzyme protection associated with high enzyme exposure to the environment, as well as a signicantly higher enzyme leaching and detachment of the enzyme during the process. Chen et al embedded the CA enzyme into a zeolitic imidazolate framework (ZIF-L) of nanoparticles to synthesize CA/ZIF-L composite.…”

Synthesis, carbonic anhydrase inhibitory activity and antioxidant activity of some 1,3‐oxazine derivatives

“…So, the design and synthesis of safe and effective CAIs as useful clinical agents is an active area of research in medicinal chemistry (Gulçin & Taslimi, ; Yiğit et al, ). Ren et al have reported the CO 2 sequestration by immobilized carbonic anhydrase on mesoporous cruciate flower‐like metal organic framework (Ren et al, ).…”