Immobilized Artificial Membrane Chromatography: Surface Chemistry and Applications

Pidgeon, Charles; Marcus, Carol S.; Alvarez, Francisco M.

doi:10.1007/978-1-4757-9235-5_16

Cited by 24 publications

(26 citation statements)

References 32 publications

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“…IAMs were shown to closely mimic the lipid environment found in cell membranes and have been used successfully in the prediction of drug transport across membranes and in the purification and reconstitution of membrane proteins (Pidgeon et al, 1992). Recently, the interaction of a pool of 100 nt RNAs containing randomized sequences with an IAM.PC.DD2 column (Regis Technologies, Inc.) prepared from phospatidylcholine analogs, was studied (Vlassov and Yarus, unpublished).…”

Section: Rna Binding By Immobilized Artificial Membranesmentioning

confidence: 98%

How was Membrane Permeability Produced in an RNA World?

Vlassov

2005

Orig Life Evol Biosph

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Darwinian evolution in an RNA World required that catalysts be encapsulated in membranes since this would allow superior catalysts to benefit from the products of their own reactions. However, typical membranes are relatively impermeable to polar and complex molecules and, thus, even primitive cells had to have RNA-based mechanisms for the uptake of external nutrients and the excretion of waste products. Nucleic acids form weak non-specific contacts with the surface of the lipid membrane in the presence of divalent cations, and strongly binding species can be obtained in the course of SELEX experiments. The only currently suggested mechanism for the production of permeability is through formation of supramolecular RNA complexes capable of destabilizing and transiently opening lipid membranes by action from one side, but neither natural nor selected examples of RNA channels or transmembrane shuttles are known so far. The necessity to evolve proteins could be strongly driven by the need to build defined hydrophobic structures that when integrated into membranes could provide selective permeability.

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Section: Rna Binding By Immobilized Artificial Membranesmentioning

confidence: 98%

How was Membrane Permeability Produced in an RNA World?

Vlassov

2005

Orig Life Evol Biosph

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“…We now report the immobilization of a nicotinic acetylcholine receptor (nAChR) subtype in the phospholipid monolayer of an immobilized artificial membrane (IAM) LC stationary phase (9) and the use of the resulting phase for the rapid on-line chromatographic determination of ligand binding affinities at a nAChR. The IAM LC stationary phase used in this study was derived from the covalent immobilization of 1-myristoyl-2-[(13-carboxyl)tridecanoyl]-sn-3-glycerophosphocholine on aminopropyl silica, and resembles one-half of a cellular membrane (9).…”

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confidence: 99%

“…The IAM LC stationary phase used in this study was derived from the covalent immobilization of 1-myristoyl-2-[(13-carboxyl)tridecanoyl]-sn-3-glycerophosphocholine on aminopropyl silica, and resembles one-half of a cellular membrane (9). nAChRs are the primary excitatory neurotransmitter receptors on skeletal muscles and on autonomic ganglia in the peripheral nervous system of vertebrates.…”

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confidence: 99%

Immobilized Nicotinic Receptor Stationary Phase for On-Line Liquid Chromatographic Determination of Drug–Receptor Affinities

Zhang

Xiao

Kellar

et al. 1998

Analytical Biochemistry

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“…85 (2002) 527 log P oct . However, in terms of hydrophobicity, the IAM surface resembles a RP-C 3 HPLC column [30] [31], whereas the LC-ABZ is a RP-C 18 stationary phase. It can, thus, be supposed that the latter provides a more hydrophobic environment for solute partitioning.…”

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confidence: 99%

Structural Properties Governing Retention Mechanisms on Immobilized Artificial Membrane (IAM) HPLC Columns

Taillardat-Bertschinger

Barbato

Quercia

et al. 2002

HCA

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The aims of this study were to investigate whether three commercially available immobilized artificial membrane (IAM) HPLC columns yield collinear data for neutral compounds, and whether IAM scales are distinct from the log P oct (partition coefficient in the octanol/H 2 O system) scale. With these objectives, the retention mechanisms on the IAM HPLC columns were analysed by linear solvation free-energy relationships (LSERs). A set of 68 neutral model compounds with known solvatochromic parameters and log P oct values was investigated, allowing a regular and broad exploration of property space. The resulting solvatochromic equations clearly indicate that the three IAM stationary phases retain small neutral solutes by a balance of intermolecular forces closely resembling those underlying partitioning in octanol/H 2 O and retention on a reversed-phase LC-ABZ HPLC column. For all systems, the solute×s size and hydrogen-bond-acceptor basicity are the two predominant factors, whereas dipolarity/polarisability and hydrogen-bond-donor acidity play only minor roles.

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Immobilized Artificial Membrane Chromatography: Surface Chemistry and Applications

Cited by 24 publications

References 32 publications

How was Membrane Permeability Produced in an RNA World?

How was Membrane Permeability Produced in an RNA World?

Immobilized Nicotinic Receptor Stationary Phase for On-Line Liquid Chromatographic Determination of Drug–Receptor Affinities

Structural Properties Governing Retention Mechanisms on Immobilized Artificial Membrane (IAM) HPLC Columns

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