Immobilized aminoacylase on porous glass beads.

Yokote, Yasuharu; Fujita, Masako; Shimura, Gen; Noguchi, Sadao; Kimura, Kazuo; Samejima, Hirotoshi

doi:10.1271/bbb1961.39.1545

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“…This can be explained by considering that at a higher SV, i.e., a shorter residence time across the hollow fiber of the Ac‐ dl ‐Met solution, the overall reaction is governed by the intrinsic enzyme reaction of the aminoacylase captured by the graft chain and not by convective mass transport of the substrate to the graft chain. In Figure 3, the conversion reported under an identical Ac‐ dl ‐Met concentration with the enzyme immobilized onto the glass beads (16) is included; the conversion by the fiber prepared in this study was about 3‐fold higher than that of the bead‐packed bed.…”

Section: Resultsmentioning

confidence: 98%

“…The permeability of the resultant aminoacylase‐immobilized hollow fiber was 80% that of the trunk polymer. This is favorable for a high rate of permeation of substrate solution at a low permeation pressure across the enzyme‐immobilized porous hollow fiber; for example, a permeation pressure of 0.01 MPa provided a space velocity (SV) of 50 h − 1 , which was about 10‐fold that of a bed packed with enzyme‐immobilized beads (16).…”

Section: Resultsmentioning

confidence: 99%

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High Conversion in Asymmetric Hydrolysis during Permeation through Enzyme-Multilayered Porous Hollow-Fiber Membranes

et al. 2001

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We describe a novel porous hollow-fiber support for immobilizing aminoacylase in multilayers. Epoxy-group-containing polymer chains were grafted onto a porous hollow-fiber membrane by radiation-induced graft polymerization of glycidyl methacrylate, and subsequently a diethylamino group as an anion-exchange group was introduced into the graft chain. Aminoacylase was adsorbed in multilayers by allowing the amioacylase buffer solution to permeate through the pores across the hollow fiber; the graft chains provided three-dimensional space for the enzymes because of their electrostatic repulsion. The adsorbed enzyme at a degree of multilayer binding of 15 was cross-linked with glutaraldehyde to prevent leakage. An acetyl-DL-methionine solution was allowed to permeate through the pores surrounded by the aminoacylase-immobilized graft chain. Production of L-methionine was observed at a 4.1 mol/h per L of the fiber for a space velocity of 200 h(-1), defined as the flow rate of the effluent penetrating the outside surface of the hollow fiber divided by the membrane volume including the lumen.

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Section: Resultsmentioning

confidence: 98%

Section: Resultsmentioning

confidence: 99%