Immobilized alcalase alkaline protease on the magnetic chitosan nanoparticles used for soy protein isolate hydrolysis

Wang, Shengnan; Zhang, Chaoran; Qi, Baokun; Sui, Xiaonan; Jiang, Lianzhou; Li, Yang; Wang, Zhongjiang; Feng, Han‐Zhong; Rui, Wang; Zhang, Qiao-Zhi

doi:10.1007/s00217-014-2301-1

Cited by 46 publications

(22 citation statements)

References 24 publications

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“…These results were comparable to the soy protein hydrolysis carried out by flavouzyme which obtained 15% DH after 6 hrs [14]. Alcalase immobilized on magnetic nano particles hydrolysed soy proteins up to 18% DH [46].…”

Section: Hydrolysis Of Proteins With Immobilized 89lsupporting

confidence: 71%

Controlled Protein Hydrolysis with Immobilized Alkaline Endo-Protease

Odaneth¹

2017

JABB

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Section: Hydrolysis Of Proteins With Immobilized 89lsupporting

confidence: 71%

Controlled Protein Hydrolysis with Immobilized Alkaline Endo-Protease

Odaneth¹

2017

JABB

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“…As GA plays a role as a spacer arm for the carriers by providing aldehyde groups to couple to free amine groups from the enzymes, forming imines, it can also act as a denaturing agent [12]. Additionally, some studies obtained different crosslinking times (between 1 h to 4 h) and GA concentrations (from 1% to 6%), implying good biocompatibility for these specific enzymes [12,21,28,29].…”

Section: Immobilisation Of Phw and Psr Onto Magnetic Nanoparticlesmentioning

confidence: 99%

“…Characteristic peaks of the immobilised enzymes attached via the crosslinker (Fig. 5C, 5D) were observed at 1,536 cm -1 , 1,544 cm -1 and 1,630 cm -1 because of the C=N and C=O absorption from the glutaraldehyde and NH2 from the enzyme [29].…”

Section: Characterization Of the Functionalized Nanoparticles Used Fomentioning

confidence: 99%

The immobilisation of proteases produced by SSF onto functionalized magnetic nanoparticles: Application in the hydrolysis of different protein sources

Yazid

Barrena

Sánchez

2016

Journal of Molecular Catalysis B: Enzymatic

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Alkaline proteases produced from protein-rich waste (hair waste and soya residues) by solid state fermentation (SSF) were immobilised onto functionalized magnetic iron oxide nanoparticles (MNPs) using glutaraldehyde as a crosslinking agent. The covalent binding method had a better immobilisation yield compared to simple adsorption, retaining 93%-96% (459±106 U/mg nanoparticles, 319±34 U/mg nanoparticles) of hair waste and soya residues proteases, respectively after crosslinking with 5% glutaraldehyde for 6 h. However, the adsorption immobilisation yield was 47%-54% after 8 h for both proteases. MNPs and immobilised proteases were characterized using transmission electron microscopy (TEM), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR) and electron diffraction. Our results indicated successful crosslinking between the proteases and amino-functionalized MNPs. The operational stability (pH and temperature) and storage stability of free and immobilised enzyme were also analysed. Despite the fact that the optimum pH of free and immobilised proteases was identical in the alkaline region, the immobilised proteases reached their optimum condition at higher temperatures (40ºC-60ºC 40 ºC-60 ºC). After 2 months of storage at 4ºC 4 ºC, the immobilised proteases showed good stability, retaining more than 85% of their initial activity. The high magnetic response of MNPs render an ease of separation and reusability, which contributes to the residual activity of both immobilised proteases on MNPs remained retaining more than 60% of their initial values after seven hydrolytic cycles. These results resulted showed the enhancement of the stability of the crosslinking interactions between the proteases and nanoparticles. The immobilised proteases were capable of hydrolysing select selected proteins (casein, oat bran protein isolate, and egg white albumin). However, differences in the degree of hydrolysis were observed, depending on the combination of the protease and type of substrate used.

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“…Besides water treatment, immobilized enzyme also shows potential in other fields like Food, medicine, cosmetics and some other industries due to its recycling convenience and stability. [17] …”

Section: Resultsmentioning

confidence: 99%

Study on Preparation of Immobilized Alkaline Protease and Its Performance

Li¹

2017

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Abstract:The nano-Fe 3 O 4 particles were prepared by hydrothermal method. Cellulose-based magnetic Fe 3 O 4 was prepared by adding cellulose solution dissolved in sodium hydroxide / thiourea / urea system. The basic protease was used as the catalyst and glutaraldehyde was used as the crosslinking agent, The production of magnetic recyclable immobilized enzyme can greatly reduce the cost of hydrolysis of sludge. The aldehyde groups in the glutaraldehyde and the amino groups in the protein can be stably crosslinked by coupling. The immobilization effect of nanometer Fe 3 O 4 was reflected by investigating the activity of immobilized enzyme and immobilization rate of enzyme under different conditions.

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Immobilized alcalase alkaline protease on the magnetic chitosan nanoparticles used for soy protein isolate hydrolysis

Cited by 46 publications

References 24 publications

Controlled Protein Hydrolysis with Immobilized Alkaline Endo-Protease

Controlled Protein Hydrolysis with Immobilized Alkaline Endo-Protease

The immobilisation of proteases produced by SSF onto functionalized magnetic nanoparticles: Application in the hydrolysis of different protein sources

Study on Preparation of Immobilized Alkaline Protease and Its Performance

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