Immobilization Techniques for the Preparation of Supported Biocatalysts: Making Better Biocatalysts Through Protein Immobilization

Rocha-Martín, Javier; Betancor, Lorena; López‐Gallego, Fernando

doi:10.1002/9783527824465.ch3

Cited by 3 publications

(2 citation statements)

References 95 publications

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“…The previously mentioned RM immobilization technology has enabled the separation of RM retention time from hydraulic retention time in environmental applications. However, the complex procedures of these immobilization processes, the time-consuming lab work to select suitable process conditions and unaffordable carriers always limited their wide applications (Rocha-Martín et al 2021;Wang et al 2021). To overcome these raised issues, data-driven approaches such as machine learning algorithms have been attempted to predict and optimize those complex procedures and to obtain feasible immobilization conditions (Shi et al 2022).…”

Section: Natural and Bio-resourced Redox Mediatormentioning

confidence: 99%

Environmental applications of immobilized and bio-resourced redox mediators: A Review

Guo

Huang

2022

BioRes

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Redox mediators (RMs), also known as electron shuttles, have been widely reported to promote both biotic and abiotic reductions of oxidized pollutants in water, soil, biogeochemical cycles, and wastewater treatment systems. However, the continuous addition of dissolved RMs is unaffordable and the potential environmental risks remain unknown because most applied RMs are synthetic chemicals. Immobilization technology enables RMs to be attached on non-dissolved supports, avoiding wash-out from the treatment systems. This realizes the reuse of RMs in scaled-up engineering applications and the in-situ remediation. Moreover, renewable natural biomass and their derivatives, such as biochar, have also aroused increased interest because they provide an economical and feasible way to solve the shortcomings of applying soluble RMs. This review presents different RM immobilization methods, which include entrapment, adsorption, and surface modification, as well as the use of bio-resourced RMs. The immobilization procedures and reaction mechanisms of the immobilized RMs and bio-resourced RMs in environmental applications are critically compared and summarized.

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Section: Natural and Bio-resourced Redox Mediatormentioning

confidence: 99%

Environmental applications of immobilized and bio-resourced redox mediators: A Review

Guo

Huang

2022

BioRes

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“…Common tasks in a cascade transformation (e.g., the recycling of co‐substrates and coenzymes; the mass flow from one reaction step to the next) are thus realized efficiently (Caparco et al, 2020 ; Velasco‐Lozano et al, 2017 ; Xu et al, 2020 ). Importantly, besides the essential features of the immobilized enzyme system (e.g., mode of protein tethering; overall protein loading; enzyme activity ratio for flux control) (Bolivar & Nidetzky, 2019 , 2020 ; Hanefeld et al, 2009 ; Rocha‐Martín et al, 2021 ; Zhong et al, 2020 ), the key characteristics of the solid carrier (e.g., surface and bulk material chemistry; external and internal hydrodynamic properties; and mechanical properties) can be tailored to the requirement of the continuous process (Bayne et al, 2013 ; Liese et al, 2013 ; Buchholz et al, 2005 ).…”

Section: Introductionmentioning

confidence: 99%

Leloir glycosyltransferases enabled to flow synthesis: Continuous production of the natural C‐glycoside nothofagin

Liu

Nidetzky

2021

Biotech & Bioengineering

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C-glycosyltransferase (CGT) and sucrose synthase (SuSy), each fused to the cationic binding module Z basic2 , were co-immobilized on anionic carrier (ReliSorb SP400) and assessed for continuous production of the natural C-glycoside nothofagin. The overall reaction was 3ʹ-C-β-glycosylation of the polyphenol phloretin from uridine 5ʹ-diphosphate (UDP)-glucose that was released in situ from sucrose and UDP.Using solid catalyst optimized for total (∼28 mg/g) as well as relative protein loading (CGT/SuSy = ∼1) and assembled into a packed bed (1 ml), we demonstrate flow synthesis of nothofagin (up to 52 mg/ml; 120 mM) from phloretin (≥95% conversion) solubilized by inclusion complexation in hydroxypropyl β-cyclodextrin. About 1.8 g nothofagin (90 ml; 12-26 mg/ml) were produced continuously over 90 reactor cycles (2.3 h/cycle) with a space-time yield of approximately 11 mg/(ml h) and a total enzyme turnover number of up to 2.9 × 10 3 mg/mg (=3.8 × 10 5 mol/mol). The coimmobilized enzymes exhibited useful effectiveness (∼40% of the enzymes in solution), with limitations on the conversion rate arising partly from external liquid-solid mass transfer of UDP under packed-bed flow conditions. The operational half-life of the catalyst (∼200 h; 30°C) was governed by the binding stability of the glycosyltransferases (≤35% loss of activity) on the solid carrier. Collectively, the current study shows integrated process technology for flow synthesis with coimmobilized sugar nucleotide-dependent glycosyltransferases, using efficient glycosylation from sucrose via the internally recycled UDP-glucose. This provides a basis from engineering science to promote glycosyltransferase applications for natural product glycosides and oligosaccharides.

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Immobilization and stabilization of enzymes using biomimetic silicification reactions

Ortiz

Jackson

Betancor

2022

J Sol-Gel Sci Technol

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Immobilization Techniques for the Preparation of Supported Biocatalysts: Making Better Biocatalysts Through Protein Immobilization

Cited by 3 publications

References 95 publications

Environmental applications of immobilized and bio-resourced redox mediators: A Review

Environmental applications of immobilized and bio-resourced redox mediators: A Review

Leloir glycosyltransferases enabled to flow synthesis: Continuous production of the natural C‐glycoside nothofagin

Immobilization and stabilization of enzymes using biomimetic silicification reactions

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