Immobilization Reduces the Activity of Surface-Bound Cationic Antimicrobial Peptides with No Influence upon the Activity Spectrum

Bagheri, Mojtaba; Beyermann, Michael; Dathe, Margitta

doi:10.1128/aac.01254-08

Cited by 223 publications

(304 citation statements)

References 60 publications

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“…Humblot et al [68] reported that the immobilized AMPs had a bacteriostatic rather that bactericidal effect, perhaps due to the low peptide concentration or short contact time. Other research has performed the immobilization through long linkers in an attempt to permit sufficient flexibility to penetrate target cell membranes [1,69,70], as the directly attached AMPs lost their antimicrobial activity. Hilpert et al [71], in their screening and characterization of surface-tethered cationic peptides studies, reported that the immobilization of peptides to a surface should result in constraints on the peptide mobility and on the capacity of peptides to enter or even transpose the cellular membranes.…”

Section: Mode Of Antibacterial Actionmentioning

confidence: 99%

“…Thus, stable immobilization of AMPs onto a biomaterial could be the pathway to overcome these difficulties [72]. Covalent immobilization of AMP can increase their long-term stability while decreasing their toxicity, as compared to incorporation approaches on leach-or releasebased systems [11,35,36,69,[72][73][74][75]. Furthermore, the proper orientation of the peptide may result in enhanced activity [34].…”

Section: Covalent Immobilization Of An Antimicrobial Peptidementioning

confidence: 99%

“…Other important activity-modulating parameters include the length, flexibility and kind of spacer between the active sequences and the solid matrices [67,69,70], the AMP surface density [1,68,69], and orientation after immobilization [1,11,[67][68][69][70][76][77][78]. These parameters are discussed below.…”

Section: Covalent Immobilization Of An Antimicrobial Peptidementioning

confidence: 99%

“…A wide variety of solid supports has been assessed for production of surfaces with immobilized AMPs, including polymeric brushes and resins [1,56,67,69,71,77,78], metal (e.g. silanized titanium) [70], glass coverslips [76], model surfaces (e.g.…”

Section: Solid Supports and Chemical Coupling Strategiesmentioning

confidence: 99%

“…Although some studies demonstrated that immobilized AMPs have antimicrobial activity without incorporation of a spacer [11,68,71,76], most protocols presented a spacer attachment step [1,56,67,69,70,77,78], particularly with a PEG spacer with a MW ranging from 3000 to 5400 [56,69,70,77,78]. The utilization of PEG as a spacer presents several advantages.…”

Section: Influence Of the Spacermentioning

confidence: 99%

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Covalent immobilization of antimicrobial peptides (AMPs) onto biomaterial surfaces

et al. 2011

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a b s t r a c tBacterial adhesion to biomaterials remains a major problem in the medical devices field. Antimicrobial peptides (AMPs) are well-known components of the innate immune system that can be applied to overcome biofilm-associated infections. Their relevance has been increasing as a practical alternative to conventional antibiotics, which are declining in effectiveness. The recent interest focused on these peptides can be explained by a group of special features, including a wide spectrum of activity, high efficacy at very low concentrations, target specificity, anti-endotoxin activity, synergistic action with classical antibiotics, and low propensity for developing resistance. Therefore, the development of an antimicrobial coating with such properties would be worthwhile. The immobilization of AMPs onto a biomaterial surface has further advantages as it also helps to circumvent AMPs' potential limitations, such as short half-life and cytotoxicity associated with higher concentrations of soluble peptides. The studies discussed in the current review report on the impact of covalent immobilization of AMPs onto surfaces through different chemical coupling strategies, length of spacers, and peptide orientation and concentration. The overall results suggest that immobilized AMPs may be effective in the prevention of biofilm formation by reduction of microorganism survival post-contact with the coated biomaterial. Minimal cytotoxicity and longterm stability profiles were obtained by optimizing immobilization parameters, indicating a promising potential for the use of immobilized AMPs in clinical applications. On the other hand, the effects of tethering on mechanisms of action of AMPs have not yet been fully elucidated. Therefore, further studies are recommended to explore the real potential of immobilized AMPs in health applications as antimicrobial coatings of medical devices.

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Section: Mode Of Antibacterial Actionmentioning

confidence: 99%

Section: Covalent Immobilization Of An Antimicrobial Peptidementioning

confidence: 99%

Section: Covalent Immobilization Of An Antimicrobial Peptidementioning

confidence: 99%

Section: Solid Supports and Chemical Coupling Strategiesmentioning

confidence: 99%

Section: Influence Of the Spacermentioning

confidence: 99%

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Covalent immobilization of antimicrobial peptides (AMPs) onto biomaterial surfaces

et al. 2011

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Polymer Brushes

Hadjesfandiari

Bajgai

et al. 2013

Encyclopedia of Polymer Science and Technology

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Polymer brushes are monolayers of one‐end tethered polymer chains at high graft density on a surface. Owing to the steric interactions between the tethered polymer chains within the brush regime, this class of polymer thin films has unique properties compared to the conventional polymer thin films. Polymer brushes provide an elegant route for surface modification owing to their excellent mechanical stability as well as functional versatility. Among the polymer brush synthesis methods, surface‐initiated polymerization (SIP) offers a unique opportunity for the generation of high graft density polymer brushes. Combined with controlled/living polymerization, the SIP method revolutionized the polymer brush synthesis and allowed the tailoring of surface properties of these molecular thin films. The control of surface properties provided new ways to change wettability, lubrication, and the interaction of biological macromolecules/cells to surfaces and created new opportunities in the design of responsive surfaces, smart materials, biocompatible surfaces, and various biotechnology and nanotechnology applications. This field is rapidly emerging with wide range of applications in multiple research fields and is promoting cross‐disciplinary research.

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