Immobilization of β-glucosidase from a commercial preparation. Part 2. Optimization of the immobilization process on chitosan

Martino, Ascanio; Pifferi, Pier Giorgio; Spagna, G.

doi:10.1016/0032-9592(95)00066-6

Cited by 79 publications

(33 citation statements)

References 10 publications

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“…Chitosan is known as an ideal natural support for enzyme immobilization because of its many characteristics such as hydrophilicity, biocompatibility, biodegradability, and antibacterial properties [1,2]. It has been used widely as an adsorbent for transition metal ions and organic species because the amino (-NH 2 ) and hydroxy (-OH) groups on chitosan chains can serve as the coordination and reaction sites [3][4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Adsorption of tannic acid, humic acid, and dyes from water using the composite of chitosan and activated clay

Chang

Juang

2004

Journal of Colloid and Interface Science

495

203

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Section: Introductionmentioning

confidence: 99%

Adsorption of tannic acid, humic acid, and dyes from water using the composite of chitosan and activated clay

Chang

Juang

2004

Journal of Colloid and Interface Science

495

203

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“…1,2 Chitosan is known as an ideal support material for enzyme immobilization because of its many advantageous characteristics such as its hydrophilicity, biocompatibility, biodegradability, and antibacterial property. [3][4][5] It has been widely used as an adsorbent for transition metals and organic species because the amino (-NH 2 ) and hydroxy (-OH) groups on chitosan chains can serve as the coordination and reaction sites. 6,7 In addition to these, chitosan appears economically attractive since chitin is the second most abundant biopolymer in nature next to cellulose.…”

Section: Introductionmentioning

confidence: 99%

Adsorption of dyes and humic acid from water using chitosan‐encapsulated activated carbon

Wu¹,

Tseng

Juang

2002

J of Chemical Tech & Biotech

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The adsorption isotherms and rates of two dyes and humic acid from aqueous solutions onto chitosan-encapsulated activated carbon (CEAC) beads were measured at 30°C. Such beads were prepared by mixing different weight percents of cuttlefish-based chitosan (100%, 80%, 67%, and 55%) and rice-based activated carbons. It was shown that the isotherms of dyes and humic acid were well fitted by the Freundlich equation. The adsorption capacity and rate could be enhanced when activated carbon was encapsulated with chitosan. Four simplified kinetic models including the pseudo-firstorder equation, pseudo-second-order equation, intraparticle diffusion model, and the Elovich equation were tested to follow the adsorption processes. The adsorption of dyes was best described by the Elovich equation, but that of humic acid was best described by the intraparticle diffusion model. The kinetic parameters of each best-fit model were calculated and are discussed in this paper. # 2002 Society of Chemical IndustryKeywords: chitosan-encapsulated activated carbon; adsorption; isotherms; kinetics; dyes; humic acidPseudo-first-order rate constant defined in eqn (7) (min À1 ) k 2 Pseudo-second-order rate constant defined in eqn (9) (kg g À1 min À1

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“…The behavior is of considerable interest for the use of the enzyme in the food-processing industries. 34 However, ENT-YADH shows an optimum pH between 8 -9.…”

Section: Resultsmentioning

confidence: 99%

Immobilization of yeast alcohol dehydrogenase by entrapment and covalent binding to polymeric supports

Soni

Desai

Devi

2001

J of Applied Polymer Sci

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Yeast alcohol dehydrogenase (YADH), which catalyzes oxidoreductions of a broad spectrum of substrates, was immobilized by entrapping it into a network of a poly(acrylamide-co-hydroxyethyl methacrylate) copolymer and was also covalently bound onto porous chitosan beads activated through glutaraldehyde. Maximum retention of YADH activity achieved was 90 and 24% for entrapment and covalent binding, respectively. The results obtained for thermal, storage, and operational stability of entrapped and covalently bound YADH were compared with free YADH. The immobilized enzyme showed improved thermal and storage stability. The immobilized enzymes also retained 50% activity after six and eight cycles. Enzyme-catalyzed oxidation of ethanol was observed to be diffusion-controlled through Lineweaver-Burk plots.

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Immobilization of β-glucosidase from a commercial preparation. Part 2. Optimization of the immobilization process on chitosan

Cited by 79 publications

References 10 publications

Adsorption of tannic acid, humic acid, and dyes from water using the composite of chitosan and activated clay

Adsorption of tannic acid, humic acid, and dyes from water using the composite of chitosan and activated clay

Adsorption of dyes and humic acid from water using chitosan‐encapsulated activated carbon

Immobilization of yeast alcohol dehydrogenase by entrapment and covalent binding to polymeric supports

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