Immobilization of yeast on delignified cellulosic material for room temperature and low-temperature wine making

Study of sugarcane pieces as yeast supports for ethanol production from sugarcane juice and molasses

“…Further, the reduction of costs, in bioprocesses involving immobilized cells systems are related to aspects such as the cost of raw materials, the use of cheap, abundant and stable immobilization supports, the high cell concentrations in the bioreactors, the simplicity and low cost of the immobilization techniques, the stability of the immobilized biocatalyst in operating conditions [6]. Based on these purposes, varies immobilization supports for variety of products have been reported such as alginates [5,7], Apple pieces [8][9][10][11], orange peel [12], gluten pellets [13] and delignified cellulosic residues [14,15]. Generally, the liginicellulose materials were delignified prior to application for the immobilization supports.…”

“…Through the delignification treatment, the lignin was removed partially and more hydrophilic groups were exposed, which increased the absorption [16]. The delignification treatment with sodium hydroxide solution increased the possibility cells going through and so accommodated their immobilization [15]. These natural biocatalysts were considered more attractive in the ethanol production as well as in wine making and beer production than inorganic supports, such as alginates, in relation with low price of the support and its abundance in nature [11].…”

Immobilization of Yeast Pichia stipitis for Ethanol Production

“…그리고, 이러한 고정화 효모 세포를 이용하는 다 양한 반응기 시스템이 연구되어 왔으며 [2,3,[8][9][10]14,26], 특히, 경제성을 고려하여 세포의 흡착을 이용한 다양한 부산물 [2,3,5,9,13,17,18,26]이 세포 고정화를 위한 담체로서 관심을 끌 고 있다. 그 중에서 woodchips, sugarcane bagasse, sorghum bagasse, 그리고 corncob과 같은 agricultural byproducts가 특 히 이러한 경제성과 간편한 방법 때문에 많이 연구되어왔다.…”

Simultaneous Production of Sugar and Ethanol from Sugarcane in China, the Development, Research and Prospect Aspects

“…Neelakantam (2004) demonstrated that a high yeast inoculation at the start of the sugarcane juice fermentation allows the yeast outgrow the contaminant bacteria and inhibit its growth and metabolism. Varies immobilization supports for variety of products have been reported such as polyvinyl alcohol (PVA, see Fig10), alginates (Kiran Sree, 2000;Corton et al, 2000), Apple pieces (Kourkoutas et al, 2006), orange peel (S.plessas, 2007), and delignified cellulosic residues (Kopsahelis, 2006;Bardi ＆ Koutinas, 1994). We applied sugarcane pieces as yeast supports for alcohol production from sugarcane juice and molasses (Fig 11).The results (Liang et al,2008) showed ethanol concentrations (about 77g/l or 89.76g/l in average value) , and ethanol productivities (about 62.76 g/l.d or 59.55g/l .d in average value)were high and stable, and residual sugar concentrations were low in all fermentations(0.3-3.6g/l)with conversions ranging from 97.7-99.8%, showing efficiency(90.2-94.2%) and operational stability of the biocatalyst for ethanol fermentation.…”