Immobilization of viral antigens on filter paper for a [125I]staphylococcal protein a immunoassay: A rapid and sensitive technique for detection of herpes simplex virus antigens and antiviral antibodies

Cleveland, Patrick H.; Richman, Douglas D.; Oxman, Michael N.; Wickham, M. Gary; Binder, Perry S.; Worthen, David M.

doi:10.1016/0022-1759(79)90008-5

Cited by 40 publications

(13 citation statements)

References 22 publications

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“…The retention of viral antigens by virus-infected cells treated with GUV or psoralen-LWUV was examined by ["2'I]SPA radioimmunoassay as described in Materials and Methods. Previous work in this laboratory has shown this method to be both sensitive and specific for the detection and quantitation of HSV antigens on infected cells (2). Under appropriate conditions, the amount of ['26I]SPA bound in the presence of a constant amount of antiviral antibody is directly proportional to the amount of virus antigen present.…”

Section: Resultsmentioning

confidence: 99%

Psoralen inactivation of influenza and herpes simplex viruses and of virus-infected cells

Redfield¹,

Richman²,

Oxman³

et al. 1981

Infect Immun

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Psoralen compounds covalently bind to nucleic acids when irradiated with longwavelength ultraviolet light. This treatment can destroy the infectivity of deoxyribonucleic acid and ribonucleic acid viruses. Two psoralen compounds, 4'-hydroxymethyltrioxsalen and 4'-aminomethyltrioxsalen, were used with long-wavelength ultraviolet light to inactivate cell-free herpes simplex and influenza viruses and to render virus-infected cells noninfectious. This method of inactivation was compared with germicidal (short-wavelength) ultraviolet light irradiation. The antigenicity of the treated, virus-infected, antigen-bearing cells was examined by immunofluorescence and radioimmunoassay and by measuring the capacity of the herpes simplex virus-infected cells to stimulate virus-specific lymphocyte proliferation. The infectivity of the virus-infected cells could be totally eliminated without altering their viral antigenicity. The use of psoralen plus long-wavelength ultraviolet light is well suited to the preparation of noninfectious virus antigens and virus antigen-bearing cells for immunological assays. Cells. Rabbit kidney (RK13) cells were obtained from

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Section: Resultsmentioning

confidence: 99%

Psoralen inactivation of influenza and herpes simplex viruses and of virus-infected cells

Redfield¹,

Richman²,

Oxman³

et al. 1981

Infect Immun

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“…The tests were based on procedures developed by Cleveland et al (1979Cleveland et al ( , 1982 and Richman et al (1981). They were performed in a 'Millititer' immunofiltration apparatus (Millipore Corp., Bedford, Mass., U.S.A.) in 96-well microplates in which the bottoms of the wells are formed by polyvinylidene fluoride membrane (Millititer SV plates).…”

Section: Radioimmunoassays (Ria)mentioning

confidence: 99%

Comparison of techniques for demonstrating antibodies to Rift Valley fever virus

Swanepoel

Struthers

Erasmus

et al. 1986

J. Hyg.

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SUMMARYNine serological techniques were compared by monitoring the response to infection with Rift Valley fever (RVF) virus in three sheep. Antibodies were monitored daily for the first 14 days after infection, then weekly and later fortnightly up to week 24. The earliest antibody response was detected in one sheep on day 3 by a plaque reduction neutralization test, and by day 6 antibodies were demonstrable in all three sheep by haemagglutination-inhibition, reversed passive haemagglutination-inhibition, immunodiffusion, indirect immunofluorescence (IF), enzyme-linked immunosorbent assay and neutralization ofcytopathic effect in cell cultures. Antibodies were demonstrable by complement fixation on day 8 at the earliest. IF and the two neutralization techniques produced the highest titres, but all tests could be used satisfactorily for the serological diagnosis of RVF. Inactivated antigen could be used for all except the neutralization tests. A radioimmunoassay technique using l251-labelled staphylococcal protein A detected antibodies on day 8 at the earliest and produced lower mean titres than some of the other techniques. This was probably because sheep immunoglobulins bind protein A poorly.

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“…The assay of antibody in microliter quantities of serum and perilymph was performed by using an enzyme immunoassay with a filtration system originally described by Cleveland et al (3) and described in detail for GPCMV elsewhere (12).…”

Section: Animalsmentioning

confidence: 99%

Ganciclovir prophylaxis for cochlear pathophysiology during experimental guinea pig cytomegalovirus labyrinthitis

Woolf

Ochi

Silva

et al. 1988

Antimicrob Agents Chemother

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The effectiveness of the antiviral agent ganciclovir (9-[1,3-dihydroxy-2-propoxymethyl]guanine) against guinea pig cytomegalovirus was tested in vitro in guinea pig embryonic fibroblasts and in vivo in an experimental guinea pig cytomegalovirus labyrinthitis model. In vitro, ganciclovir completely prevented guinea pig cytomegalovirus infection of guinea pig embryonic fibroblasts at concentrations above 32.6 ,ug/ml. In vivo, antibody-negative animals had an average 17-dB elevation in their auditory nerve compound action potential thresholds (P < 0.01, t test) and showed signs bilaterally of guinea pig cytomegalovirus labyrinthitis 8 days after intrathecal inoculation of virus. Ganciclovir administration starting 1 day before inoculation prevented the development of both cochlear histopathologic change and hearing loss. Guinea pig cytomegalovirus meningitis was observed in both the drug-treated and untreated groups. High-pressure liquid chromatography confirmed the presence of ganciclovir in the serum, perilymph, and cerebrospinal fluid of the drug recipients. Prophylactic ganciclovir thus can protect the cochlea from the histopathologic changes and hearing loss normally associated with experimental guinea pig cytomegalovirus labyrinthitis.Cytomegalovirus (CMV) is the leading infectious cause of congenital sensorineural hearing loss in humans. As many as 2.5% of all newborns have been estimated to be congenitally infected with CMV (10,11,13,25,26). About 5% of congenitally infected infants develop symptomatic congenital CMV syndrome. One half of these infants die, and the survivors typically exhibit significant and permanent intellectual, auditory, visual, or neurologic sequellae of their infection (26). Ninety-five percent of infants congenitally infected with CMV are asymptomatic at birth. However, as many as 20% of infants who appear normal at birth are later found to have sensorineural deafness, neurologic abnormalities, or mental retardation (11,18). Recent epidemiological studies have concluded that in the United States alone both congenital CMV infections and primary CMV infections later in life may account for as many as 40,000 new cases of sensorineural hearing loss per year (6). CMV may also be a significant factor in the sensorineural hearing loss and central auditory dysfunction found in up to 68% of patients with acquired immunodeficiency syndrome (J. Gardi, C. D. Sooy, D. Morledge, P. Chambers, R. Gorter, and I. Medina, Assoc. Res. Otolaryngol. 10:93, 1987), over 90% of whom are infected with CMV (20). The pathogenesis of CMVinduced sensorineural hearing loss is poorly understood, and there is no known effective treatment.The species specificity of CMV has prevented the development of animal models using human CMV. However, it was recently demonstrated that the virology and histopathology of guinea pig CMV (GPCMV) closely resemble those of human CMV disease (2, 27). Using GPCMV, we established a model of virus labyrinthitis in guinea pigs after inoculation of seronegative animals with GPCMV via intra...

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Immobilization of viral antigens on filter paper for a [125I]staphylococcal protein a immunoassay: A rapid and sensitive technique for detection of herpes simplex virus antigens and antiviral antibodies

Cited by 40 publications

References 22 publications

Psoralen inactivation of influenza and herpes simplex viruses and of virus-infected cells

Psoralen inactivation of influenza and herpes simplex viruses and of virus-infected cells

Comparison of techniques for demonstrating antibodies to Rift Valley fever virus

Ganciclovir prophylaxis for cochlear pathophysiology during experimental guinea pig cytomegalovirus labyrinthitis

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