Immobilization of vaginal Lactobacillus in polymeric nanofibers for its incorporation in vaginal probiotic products

Probiotic lactobacilli in formulas and hygiene products for the health of the urogenital tract

“…On the other hand, nanotechnology applying the electrospinning technique has recently been proposed to cover different devices for vaginal uses with viable Lactobacillus in a single step. 178,179 The coating system was successfully applied in several products, as stent, soybeans, cellulose paper, with a wide variety of bioactive immobilized in nanofibers through electrospinning. [180][181][182] Then, this Lactobacillus immobilization system offers advantages when compared with lyophilized powders, since modifications of the final product are not evidenced, and subsequent processing for their incorporation in the product design are not required.…”

“…On the other hand, nanotechnology applying the electrospinning technique has recently been proposed to cover different devices for vaginal uses with viable Lactobacillus in a single step 178,179 . The coating system was successfully applied in several products, as stent, soybeans, cellulose paper, with a wide variety of bioactive immobilized in nanofibers through electrospinning 180–182 .…”

“…The immobilization of a L . rhamnosus CRL 1332 vaginal strain in polyvinylalcohol nanofibers by electrospinning was successfully, maintaining the required viable cell numbers during 360 days at 4°C and the inhibition to urogenital pathogens 178 . Up to date, clinical trials to determine the safety, efficacy and effectiveness of immobilized probiotics in these products for UGTI treatment were not published.…”

Advancements in the Pharmaceutical Applications of Probiotics: Dosage Forms and Formulation Technology

“…Nanofibers exhibit uniform morphology and composition, nanometric diameter, large surface areas, and high porosity, offering many advantages for the efficient delivery of biological substances. 153 Consequently, nanofibers can be used to encapsulate various microorganisms, cells, genes, and proteins. 152 , 153 The incorporation of probiotics into nanofibers can improve the stability of probiotic bacterial cells and also allow their site-specific delivery.…”

“… 153 Consequently, nanofibers can be used to encapsulate various microorganisms, cells, genes, and proteins. 152 , 153 The incorporation of probiotics into nanofibers can improve the stability of probiotic bacterial cells and also allow their site-specific delivery. López-Rubio et al prepared ultrathin PVA-based electrospun fibers encapsulating Bifidobacterium animalis Bb12 ( Figure 3 ), 154 which had a mean diameter of 150 nm and were effective in retaining the viability of encapsulated probiotics for 40 days of storage at room temperature and for 130 days under the refrigerated conditions.…”