Immobilization of uricase within polystyrene using polymaleimidostyrene as a stabilizer and its application to uric acid sensor

Wang, Xiuyun; Hagiwara, Tokio; Uchiyama, Shunichi

doi:10.1016/j.aca.2007.01.025

Cited by 39 publications

(27 citation statements)

References 16 publications

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“…As a contrast on the correctness of the results, the UA concentrations of the same samples were analyzed simultaneously by the HPLC method. The results summarized in Table 1 show that the UA concentrations found in urine samples are fairly close to those reported elsewhere, [19][20][21][22][23] and the results of HPLC. 9 …”

Section: Analysis Of Urine Samplessupporting

confidence: 74%

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Highly Sensitive and Selective Uric Acid Biosensor Based on Direct Electron Transfer of Hemoglobin-encapsulated Chitosan-modified Glassy Carbon Electrode

et al. 2009

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A uric acid biosensor based on the direct electron transfer of a hemoglobin-encapsulated chitosan-modified glassy carbon electrode was developed for a highly sensitive and selective analysis in urine samples. The modified electrode was prepared by the encapsulation of hemoglobin and uricase in a chitosan matrix. The hydrogen peroxide produced from the catalytic oxidation of uric acid by uricase was reduced electrocatalytically by immobilized hemoglobin and used to obtain a sensitive amperometric response to uric acid. The linear response of the uric acid concentrations ranged from 2.00 to 30.0 µM with a correlation of 0.9982, the detection limit of uric acid was estimated to be 0.85 µM at a signal/noise ratio of 3. The uric acid biosensor can efficiently exclude the interference of commonly coexisted ascorbic acid, dopamine, epinephrine, etc. The relative standard deviation was under 2.56% (n = 5) for the determination of real samples. This biosensor is satisfactory for the determination of human urine samples compared with the HPLC-UV method.

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Section: Analysis Of Urine Samplessupporting

confidence: 74%

“…[20][21][22] However, the interferences contributed by other electroactive species, such as ascorbic acid (AA), epinephrine (EP) and dopamine (DA) often disturb the proper determination of UA. Also, the reported amperometric biosensor can only achieve a relative high detection limit of about 2.0 µM.…”

Section: Introductionmentioning

confidence: 99%

Highly Sensitive and Selective Uric Acid Biosensor Based on Direct Electron Transfer of Hemoglobin-encapsulated Chitosan-modified Glassy Carbon Electrode

et al. 2009

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“…Several biosensors based on uricase had been reported [11][12][13][14]. However, they still have some disadvantages which include sensitivity to O 2 concentrations, significant interference signals by ascorbic acid, etc.…”

Section: Introductionmentioning

confidence: 99%

Non-enzymatic disposable test strip for detecting uric acid in whole blood

Chen

Fang

Qiu

et al. 2009

Journal of Electroanalytical Chemistry

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“…Table 1 summarizes the work carried out on uric acid biosensors by various workers using different matrices. Various matrices have been utilized for the detection of uric acid such as APTES Bis sulfosuccinimide/ITO [3], gold electrode/polystyrene [4], carbon felt based H 2 O 2 [5], poly N-isopropyl acrylamide MWCNT-Ch/poly(amidoamine)/DNA/gold electrode [6], Polyaniline [7], screen printed electrode [8] and so on. However, the low sensitivity and poor shelf life of the biosensors are major concern.…”

Section: Introductionmentioning

confidence: 99%

Modified Tin Oxide Based Bioelectrode for Reagentless Detection of Uric Acid

Arora¹,

Tomar²,

Gupta³

2017

J Biosens Bioelectron

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A reagentless uric acid biosensor has been realized using Copper implanted tin oxide thin film (Cu:SnO 2 ) based matrix. The biosensing characteristics of implanted matrix have been studied using the electrochemical impedance spectroscopy and cyclic voltammetry. The prepared matrix (Cu:SnO 2 ), because of the presence of Cu, possess redox properties so that the electron transfer from enzyme to the electrode could be accomplished without using any external mediator in the electrolyte. The developed uric acid biosensor exhibits a high sensitivity of about 0.93 mA/ mM and a linear variation in current response over a concentration range from 0.05 to 1.0 mM of uric acid besides high shelf life (~20 weeks). The Michaelis-Menten kinetic parameter (K m ) is found to be relatively very low (0.12 mM), which indicates the high affinity of the fabricated bioelectrode towards the uric acid (analyte). The results highlight the importance of implanted Cu:SnO 2 thin film as an attractive matrix for the realization of reagentless biosensors towards uric acid.

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Immobilization of uricase within polystyrene using polymaleimidostyrene as a stabilizer and its application to uric acid sensor

Cited by 39 publications

References 16 publications

Highly Sensitive and Selective Uric Acid Biosensor Based on Direct Electron Transfer of Hemoglobin-encapsulated Chitosan-modified Glassy Carbon Electrode

Highly Sensitive and Selective Uric Acid Biosensor Based on Direct Electron Transfer of Hemoglobin-encapsulated Chitosan-modified Glassy Carbon Electrode

Non-enzymatic disposable test strip for detecting uric acid in whole blood

Modified Tin Oxide Based Bioelectrode for Reagentless Detection of Uric Acid

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