Immobilization of Thiobacillus ferrooxidans using various polymers as matrix.

Wakao, Norio; Endo, Kazuhiko; Mino, Kenichi; Sakurai, Yonekichi; Shiota, Hideo

doi:10.2323/jgam.40.349

Cited by 21 publications

(7 citation statements)

References 9 publications

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“…Entrapment of ¹. ferrooxidans within calcium alginate, agar, -carrageenan, gerlite and photocrosslinkable resin has been reported in the literature (Lancy and Tuovinen 1984;Fig. 1 Scanning electron micrographs of a fresh polyurethane foam biomass support particle Wakao et al 1994). The concept of biomass support particles (BSP), introduced and patented by Atkinson et al (1978Atkinson et al ( , 1980, involves providing a structure within which the organism may grow, protected from high external shear and within which even weakly adhesive or flocculent organisms will be retained.…”

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confidence: 97%

Effect of ferrous iron concentration on the catalytic activity of immobilized cells of Thiobacillus ferrooxidans

Nemati

Webb

1996

Applied Microbiology and Biotechnology

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The kinetics of continuous oxidation of ferrous iron by immobilized cells of ¹hiobacillus ferrooxidans was studied in a packed-bed bioreactor. Polyurethane foam biomass support particles were used as carriers for cell immobilization. Effects of ferrous iron concentration and its volumetric loading on the kinetics of the reaction were investigated. Media containing different concentrations of ferrous iron in the range 5-20 kg m\ were tested. For each medium the kinetics of the reaction at different volumetric loadings of ferrous iron, at a constant temperature of 30°C, were determined. With media containing 5 kg m\ and 10 kg m\ Fe>, the fastest oxidation rates of 34.25 kg m\ h\ and 32 kg m\ h\ were achieved at a dilution rate of around 6 h\, which represents a residence time of 10 min. Employing a higher concentration of ferrous iron (20 kg m\) in the medium resulted in lower oxidation rates, with a maximum value of 10 kg m\ h\, indicating an inhibitory effect of ferrous iron on growth and activity of ¹. ferrooxidans. The reliable performance of the bioreactor during the course of the experiments confirmed the suitability of polyurethane foam biomass support particles as carriers for ¹. ferrooxidans immobilization.

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confidence: 97%

Effect of ferrous iron concentration on the catalytic activity of immobilized cells of Thiobacillus ferrooxidans

Nemati

Webb

1996

Applied Microbiology and Biotechnology

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“…Whole cell entrapment in calcium alginate, K-carrageenan and gerlite as gel matrix have also been attempted. 15,16 Cell entrapment in PVA cryogel as plain carrier 2,3 and in combination with boric acid, 17 with sodium alginate 18 and also passive cell attachment on siliceous stone particles, 19 ceramic beads 20 have been reported as good packed-bed supports for Acidithiobacillus ferrooxidans immobilization. The effect of surface roughness of active carbon fibers on immobilization of Acidithiobacillus ferrooxidans in inverse fluidized bed biofilm reactor 21 and the influence of different process variables on bio-oxidation by Acidithiobacillus ferrooxidans supported on LDPE particles in packed bed 22 have been recently reported.…”

Section: Introductionmentioning

confidence: 99%

Ferrous iron oxidation by foam immobilized Acidithiobacillus ferrooxidans: Experiments and modeling

Jaisankar

Modak

2009

Biotechnology Progress

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Ferrous iron bio-oxidation by Acidithiobacillus ferrooxidans immobilized on polyurethane foam was investigated. Cells were immobilized on foams by placing them in a growth environment and fully bacterially activated polyurethane foams (BAPUFs) were prepared by serial subculturing in batches with partially bacterially activated foam (pBAPUFs). The dependence of foam density on cell immobilization process, the effect of pH and BAPUF loading on ferrous oxidation were studied to choose operating parameters for continuous operations. With an objective to have high cell densities both in foam and the liquid phase, pretreated foams of density 50 kg/m(3) as cell support and ferrous oxidation at pH 1.5 to moderate the ferric precipitation were preferred. A novel basket-type bioreactor for continuous ferrous iron oxidation, which features a multiple effect of stirred tank in combination with recirculation, was designed and operated. The results were compared with that of a free cell and a sheet-type foam immobilized reactors. A fivefold increase in ferric iron productivity at 33.02 g/h/L of free volume in foam was achieved using basket-type bioreactor when compared to a free cell continuous system. A mathematical model for ferrous iron oxidation by Acidithiobacillus ferrooxidans cells immobilized on polyurethane foam was developed with cell growth in foam accounted by an effectiveness factor. The basic parameters of simulation were estimated using the experimental data on free cell growth as well as from cell attachment to foam under nongrowing conditions. The model predicted the phase of both oxidation of ferrous in shake flasks by pBAPUFs as well as by fully activated BAPUFs for different cell loadings in foam. Model for stirred tank basket bioreactor predicted within 5% both transient and steady state of the experiments closely for the simulated dilution rates. Bio-oxidation at high Fe(2+) concentrations were simulated with experiments when substrate and product inhibition coefficients were factored into cell growth kinetics.

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“…The constant value was probably reached because the new iron(III) deposits preclude nutrient access. However, the iron(III) productivity and the final soluble iron are not sufficiently high in comparison with values reported (5-50 mmol .l -1.h -1 ) using other supports under similar, but not identical, conditions [16,22,24]. When the reactor operating in repeated batch mode reached its highest iron(III) productivity, the culture medium was replaced by fresh media and a continuous flow of medium through a peristaltic pump was started.…”

Section: Resultsmentioning

confidence: 92%

“…Different immobilization methods and several supports have been employed, including glass beads, activated carbon particles, sand, polystyrene, polyurethane, poly(vinyl alcohol), calcium alginate, ion-exchange resin, nickel alloy fibre, PVC and diatomaceous earth [8][9][10][11][12][13][14][15][16][17]. A. ferrooxidans cells immobilized on supports can grow at higher dilution rates and reach high iron(III) productivity.…”

Section: Introductionmentioning

confidence: 99%

Ferrous Iron Oxidation by Immobilized on Refractory Clay Tiles

Donati¹

2008

TOBIOTJ

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Refractory clay tiles are composed of kaolin, which is the commercial name of clay, and this consists mainly of kaolinite mineral. Two such tiles and caowool packed in glass columns were used for the immobilization of Acidithiobacillus ferrooxidans cells in a ferrous iron medium, which was percolated through the supports. Colonization was carried out by several media replacements with no further inoculation until maximum ferric iron productivity was reached. One of the tiles was discarded due to the high iron precipitation during bacterial growth. The columns with the other supports were used for ferrous iron oxidation in batch and continuous flow modes of operation and these appeared to be promising supports for A. ferrooxidans. A ferrous iron oxidation rate of 14.5 mmol.l -1 .h -1 was reached in one of the columns in the continuous culture. After being used for several cultures, pieces of tiles with immobilized cells were stored at 4 ºC. Samples at different times were incubated in ferrous medium and these showed high cell activity even after 6 months.

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Immobilization of Thiobacillus ferrooxidans using various polymers as matrix.

Cited by 21 publications

References 9 publications

Effect of ferrous iron concentration on the catalytic activity of immobilized cells of Thiobacillus ferrooxidans

Effect of ferrous iron concentration on the catalytic activity of immobilized cells of Thiobacillus ferrooxidans

Ferrous iron oxidation by foam immobilized Acidithiobacillus ferrooxidans: Experiments and modeling

Ferrous Iron Oxidation by Immobilized on Refractory Clay Tiles

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