Immobilization of Thermomyces lanuginosus lipase on ZnO nanoparticles: mimicking the interfacial environment

Shah, Ekta; Mahapatra, Paramita; Bedekar, Ashutosh V.; Soni, Hemant

doi:10.1039/c5ra02249e

Cited by 23 publications

(11 citation statements)

References 53 publications

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“…Zinc oxide, as a versatile nanomaterial, has been proved suitable for biotechnological applications by plenty of studies. − There are also several works dealing with enzyme immobilization on ZnO nanoparticles, , while these structures are on the list of the U.S. Food and Drug Administration (21CFRI82.8991) as safe (GRAS). β-Glucosidases (E.C 3.2.1.21) are well-characterized biological catalysts .…”

Section: Introductionmentioning

confidence: 99%

Development of a ZnO Nanowire Continuous Flow Microreactor with β-Glucosidase Activity: Characterization and Application for the Glycosylation of Natural Products

Gkantzou

Govatsi

Chatzikonstantinou

et al. 2021

ACS Sustainable Chem. Eng.

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Microreactor technology is being increasingly utilized in the field of biocatalysis, offering cost-effectiveness and environmental sustainability. On the other hand, enzyme immobilization on nanomaterials can provide biocatalytic systems with the operational stability demands of an intensified bioprocess. Combining the methodology for ZnO nanowire growth on glass surfaces with enzyme immobilization techniques, we developed a novel continuous-flow microbioreactor system, with β-glucosidase activity. Morphological, spectroscopic, and biocatalytic characterization of the developed enzyme microreactor is presented. The immobilized enzyme exhibited almost 100% remaining hydrolytic activity after 1000 cycles of continuous use and more than 70% residual activity after 24 h of exposure to different organic solvents. The system productivity was enhanced up to 10 times compared to the free enzyme form and 30 times compared to the batch immobilized system. Compared to previous studies in batch reactor systems, the enzyme microbioreactor exhibited a productivity enhancement of 315 and 12 times, respectively, for the continuous glycosylation of the natural compounds perillyl alcohol and tyrosol toward the synthesis of their bioactive glycosylated derivatives. The entire arrangement seems to provide a proper microenvironment for enzyme stabilization, under the scope to broaden the biocatalyst’s potentiality.

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Section: Introductionmentioning

confidence: 99%

Development of a ZnO Nanowire Continuous Flow Microreactor with β-Glucosidase Activity: Characterization and Application for the Glycosylation of Natural Products

Gkantzou

Govatsi

Chatzikonstantinou

et al. 2021

ACS Sustainable Chem. Eng.

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“…Zinc oxide (ZnO) nanoparticles have been exploited in many fields such as catalysis, drug delivery and biology, where they have unique ability to promote faster electron transfer between the active site of enzymes and substrates, as a potential material in enzyme engineering, however, very little information about their application in immobilization of lipase can be found [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

Preparation of Carriers Based on ZnO Nanoparticles Decorated on Graphene Oxide (GO) Nanosheets for Efficient Immobilization of Lipase from Candida rugosa

et al. 2017

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Herein, a promising carrier, graphene oxide (GO) decorated with ZnO nanoparticles, denoted as GO/ZnO composite, has been designed and constructed. This carrier was characterized by X-ray powder diffraction, scanning electron microscopy, Fourier transform infrared spectroscopy and thermogravimetry. Then, Candida rugosa lipase (CRL) was immobilized onto the GO-based materials via physical adsorption. Our results indicated that the lipase loading amount on the GO/ZnO composites was about 73.52 mg of protein per g. In the activity assay, the novel immobilized lipase GO/ZnO@CRL, exhibited particularly excellent performance in terms of thermostability and reusability. Within 30 min at 50 °C, the free lipase, GO@CRL and ZnO@CRL had respectively lost 64%, 62% and 41% of their initial activity. However, GO/ZnO@CRL still retained its activity of 63% after 180 min at 50 °C. After reuse of the GO/ZnO@CRL 14 times, 90% of the initial activity can be recovered. Meanwhile, the relative activity of GO@CRL and ZnO@CRL was 28% and 23% under uniform conditions. Hence, GO-decorated ZnO nanoparticles may possess great potential as carriers for immobilizing lipase in a wide range of applications.

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“…Improved activity of immobilized lipase at elevated temperatures is considered as a major advantage for large scale applications, indicating better temperature adaptability. Studies have reported a similar pattern regarding the immobilization of TLL on different nanomaterials, such as magnetic [ 54 ] and ZnO-coated nanoparticles [ 55 ].…”

Section: Resultsmentioning

confidence: 84%

Green Synthesized Magnetic Nanoparticles as Effective Nanosupport for the Immobilization of Lipase: Application for the Synthesis of Lipophenols

et al. 2021

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In this work, hybrid zinc oxide–iron oxide (ZnOFe) magnetic nanoparticles were synthesized employing Olea europaea leaf aqueous extract as a reducing/chelating and capping medium. The resulting magnetic nanoparticles were characterized by basic spectroscopic and microscopic techniques, namely, X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), fourier-transform infrared (FTIR) and atomic force microscopy (AFM), exhibiting a spherical shape, average size of 15–17 nm, and a functionalized surface. Lipase from Thermomyces lanuginosus (TLL) was efficiently immobilized on the surface of ZnOFe nanoparticles through physical absorption. The activity of immobilized lipase was found to directly depend on the enzyme to support the mass ratio, and also demonstrated improved pH and temperature activity range compared to free lipase. Furthermore, the novel magnetic nanobiocatalyst (ZnOFe-TLL) was applied to the preparation of hydroxytyrosyl fatty acid esters, including derivatives with omega-3 fatty acids, in non-aqueous media. Conversion yields up to 90% were observed in non-polar solvents, including hydrophobic ionic liquids. Different factors affecting the biocatalyst performance were studied. ZnOFe-TLL was reutilized for eight subsequent cycles, exhibiting 90% remaining esterification activity (720 h of total operation at 50 °C). The green synthesized magnetic nanoparticles, reported here for the first time, are excellent candidates as nanosupports for the immobilization of enzymes with industrial interest, giving rise to nanobiocatalysts with elevated features.

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Immobilization of Thermomyces lanuginosus lipase on ZnO nanoparticles: mimicking the interfacial environment

Abstract: In this study, we propose that enzyme activity on immobilization can be controlled and enhanced by providing the environment mimicking the lipid/water interface.

Cited by 23 publications

References 53 publications

Development of a ZnO Nanowire Continuous Flow Microreactor with β-Glucosidase Activity: Characterization and Application for the Glycosylation of Natural Products

Development of a ZnO Nanowire Continuous Flow Microreactor with β-Glucosidase Activity: Characterization and Application for the Glycosylation of Natural Products

Preparation of Carriers Based on ZnO Nanoparticles Decorated on Graphene Oxide (GO) Nanosheets for Efficient Immobilization of Lipase from Candida rugosa

Green Synthesized Magnetic Nanoparticles as Effective Nanosupport for the Immobilization of Lipase: Application for the Synthesis of Lipophenols

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