Immobilization of thermolysin enzyme on dendronized silica supports. Evaluation of its feasibility on multiple protein hydrolysis cycles

Hernández-Corroto, Ester; Sánchez-Milla, María; Sánchez‐Nieves, Javier; Marina, Marı́a Luisa; García, María Concepción

doi:10.1016/j.ijbiomac.2020.10.138

Cited by 9 publications

(12 citation statements)

References 56 publications

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“…This pyrolysis can generate N-containing inert gas, which can dilute the oxygen concentration and quench the essential active free radicals . Finally, the flame is hindered by terminating the chain reaction from burning in the gas phase …”

Section: Results and Discussionmentioning

confidence: 99%

“…42 Finally, the flame is hindered by terminating the chain reaction from burning in the gas phase. 9 Based on the aforementioned discussion, PAMAM dendrimers can be regarded as an outstanding flame retardant and curing agent. Obviously, its flame retardant mechanism is expressed in both the gas phase and condensed phase.…”

Section: Acs Appliedmentioning

confidence: 99%

“…These non-flammable gases can dilute oxygen concentration during combustion, and therefore, it can work as a flame retardant. Furthermore, the PAMAM dendrimer is a class of branched monodispersed synthetic macromolecules with a variety of primary and tertiary amino groups, and these amino groups can provide a large number of active sites. − Besides, it is found that the third-generation PAMAM(G3) dendrimer has the highest molecular density and a large internal cavity structure . The cavity structure is greatly favorable for polymer penetration. , Meanwhile, the PAMAM dendrimer has a high number of amino groups on its molecular surface, and these functional groups can easily crosslink with polymers, and thus, it could be used as a curing agent for thermosetting resins.…”

Section: Introductionmentioning

confidence: 99%

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Poly(amidoamine) Dendrimers: An Excellent Intrinsic Flame Retardant and Curing Agent for Epoxy Resins

Yang

et al. 2023

ACS Appl. Polym. Mater.

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Considerable efforts have been made to fabricate the intrinsic epoxy resin (EP) with excellent flame retardancy and mechanical properties. However, conventional single molecule flame retardants have met the bottleneck after the massive research studies of the past decade, and with the development of the polymer technology, dendritic flame retardants have been studied due to their versatility and more efficient flame retardancy. Herein, poly-amidoamine (PAMAM) dendrimer flame retardants of different generations were synthesized and utilized as a curing agent and a flame retardant to fabricate the intrinsic EP materials. Their impacts on integrated properties of an EP matrix were studied thoroughly. As expected, the thermal stability, the fire safety, and the flexural capacity of EP/PAMAM thermosets were highly strengthened. The results showed that with 20 wt % loading content of the third-generation PAMAM(G3) dendrimer in the EP matrix (named as EP/20G3), its limited oxygen index value reached up to 26.4% and passed the UL-94 V-0 level. The peak heat release rate and the total heat release data decreased by 51.1 and 60.3%, respectively. Furthermore, the values of T g , flexural strength, and flexural modulus of EP/20G3 were also promoted comparing with that of the pure EP. At the same time, as an amino-abundant compound, PAMAM dendrimers with different generations can also be served as a curing agent for EP instead of the commonly used diethylenetriamine. It means that PAMAM dendrimers play a dual role in the preparation of flame retardant EP in this system.

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Section: Results and Discussionmentioning

confidence: 99%

Section: Acs Appliedmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Poly(amidoamine) Dendrimers: An Excellent Intrinsic Flame Retardant and Curing Agent for Epoxy Resins

Yang

et al. 2023

ACS Appl. Polym. Mater.

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“…81 These limitations can be overcome by the immobilization of enzymes. Recently, Hernandez-Corroto et al 82 have demonstrated first the feasibility of silica supports functionalized with different generations of PAMAM and carbosilane dendrimers for the immobilization of enzymes. Thermolysin immobilized on G0 dendrimer supports showed the highest activity (78% of the free enzyme activity), which could be employed in three consecutive hydrolysis cycles.…”

Section: Preparation Of Sweet Peptidesmentioning

confidence: 99%

Sweet Taste Receptors and Associated Sweet Peptides: Insights into Structure and Function

Zhao,

Zheng,

et al. 2023

J. Agric. Food Chem.

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“…The multifunctionality of dendrimers can be very useful to control the immobilization of enzymes to surfaces and drive away enzyme from material support to avoid undesired interactions (Sheldon & Pelt, 2013). Silica modified with amine-functional CBS dendrimers were used for grafting thermolysin and the hydrolysis ability of the new system was explored (Hernandez-Corroto et al, 2020). Although thermolysin hydrolyzed BSA, due to the hydrophobic character of CBS framework, peptides released in this process were retained by the modified material making difficult the applicability of this immobilized enzyme.…”

Section: Ppotein Separationmentioning

confidence: 99%

Carbosilane dendritic nanostructures, highly versatile platforms for pharmaceutical applications

Mata

Gomez-Ramirez

Cano

et al. 2022

WIREs Nanomed Nanobiotechnol

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Dendrimers are multifunctional molecules with well‐defined size and structure due to the step‐by‐step synthetic procedures required in their preparation. Dendritic constructs based on carbosilane scaffolds present carbon–carbon and carbon–silicon bonds, which results in stable, lipophilic, inert, and flexible structures. These properties are highly appreciated in different areas, including the pharmaceutical field, as they can increase the interaction with cell membranes and improve the therapeutic action. This article summarizes the most recent advances in the pharmaceutical applications of carbosilane dendritic molecules, from therapeutics to diagnostics and prevention tools. Dendrimers decorated with cationic, anionic, or other moieties, including metallodendrimers; supramolecular assemblies; dendronized nanoparticles and surfaces; as well as dendritic networks like hydrogels are described. The collected examples confirm the potential of carbosilane dendrimers and dendritic materials as antiviral or antibacterial agents; in therapy against cancer, neurodegenerative disease, or oxidative stress; or many other biomedical applications. This article is categorized under: Nanotechnology Approaches to Biology > Nanoscale Systems in Biology Therapeutic Approaches and Drug Discovery > Nanomedicine for Infectious Disease Therapeutic Approaches and Drug Discovery > Nanomedicine for Oncologic Disease

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Immobilization of thermolysin enzyme on dendronized silica supports. Evaluation of its feasibility on multiple protein hydrolysis cycles

Cited by 9 publications

References 56 publications

Poly(amidoamine) Dendrimers: An Excellent Intrinsic Flame Retardant and Curing Agent for Epoxy Resins

Poly(amidoamine) Dendrimers: An Excellent Intrinsic Flame Retardant and Curing Agent for Epoxy Resins

Sweet Taste Receptors and Associated Sweet Peptides: Insights into Structure and Function

Carbosilane dendritic nanostructures, highly versatile platforms for pharmaceutical applications

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