Immobilization of rhodopsin on a self-assembled multilayer and its specific detection by electrochemical impedance spectroscopy

Hou, Yanxia; Helali, Salwa; Zhang, Aidong; Jaffrézic‐Renault, Nicole; Martelet, C.; Minic, Jasmina; Gorojankina, Tatiana; Persuy, Marie‐Annick; Pajot‐Augy, Edith; Salesse, Roland; Bessueille, François; Samitier, J.; Errachid, Abdelhamid; Akimov, V.; Reggiani, L.; Pennetta, C.; Alfinito, Eleonora

doi:10.1016/j.bios.2005.06.002

Cited by 102 publications

(56 citation statements)

References 35 publications

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“…Furthermore, a significative conformational change is identified both with the onenode AA model and with the two-nodes AB models, the latter foreseing significantly larger differences among the configurations. These results are supported by some experimental evidences [25]: Bovine rhodopsin and the rat olfactory receptor I7 has been immobilized on a gold electrode building up a self-assembled multilayer. The electrochemical characteristic of these structures has been performed in a standard electrochemical cell, and has shown Nyquist plots qualitatively similar to those obtained in the present paper.…”

Section: Discussionsupporting

confidence: 70%

“…(1), we have calculated the frequency response of the impedance network, Z(f ), in the frequency range 0÷1100 Hz, and represented it by means of the Nyquist plot. This kind of plot is very used in signal processing and in characterizing the electrical properties of biological materials appropriately deposited on functionalized gold electrodes [25]. It combines the imaginary and real part of a transfer function, in our case the impedance associated with the protein, using the frequency as an implicit variable.…”

Section: B the Impedance Networkmentioning

confidence: 99%

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A network model to correlate conformational change and the impedance spectrum of single proteins

2008

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One of the main trend in to date research and development is the miniaturization of electronic devices. In this perspective, integrated nanodevices based on proteins or biomolecules are attracting a major interest. In fact, it has been shown that proteins like bacteriorhodopsin and azurin, manifest electrical properties which are promising for the development of active components in the field of molecular electronics. Here we focus on two relevant kinds of proteins: The bovine rhodopsin, prototype of GPCR protein, and the enzyme acetylcholinesterase (AChE), whose inhibition is one of the most qualified treatments of Alzheimer disease. Both these proteins exert their functioning starting with a conformational change of their native structure. Our guess is that such a change should be accompanied with a detectable variation of their electrical properties. To investigate this conjecture, we present an impedance network model of proteins, able to estimate the different electrical response associated with the different configurations. The model resolution of the electrical response is found able to monitor the structure and the conformational change of the given protein. In this respect, rhodopsin exhibits a better differential response than AChE. This result gives room to different interpretations of the degree of conformational change and in particular supports a recent hypothesis on the existence of a mixed state already in the native configuration of the protein.

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Section: Discussionsupporting

confidence: 70%

Section: B the Impedance Networkmentioning

confidence: 99%

A network model to correlate conformational change and the impedance spectrum of single proteins

2008

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“…Plot of peak current vs. square root of scan rate, obtained for 1 mM AA in pH 6.0 0.2 M phosphate buffer: ( ) bare GCE and after modification: ( ) MONO modified GCE, ( ) PAG modified GCE and ( ) PAG/GLU modified GCE. of the electrochemical reactions [34]. The EIS measurements were performed in 1 mM AA pH 6.0 0.1 M phosphate buffer solution using the bare GCE and poly(glutamic acid) modified GCE (Fig.…”

Section: Electrochemical Impedance Spectroscopy Of Ascorbic Acid On Tmentioning

confidence: 99%

Poly(glutamic acid) nanofibre modified glassy carbon electrode: Characterization by atomic force microscopy, voltammetry and electrochemical impedance

Santos

Zanoni

Bergamini

et al. 2008

Electrochimica Acta

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Glassy carbon electrodes (GCE) were modified with poly(glutamic acid) acid films prepared using three different procedures: glutamic acid monomer electropolymerization (MONO), evaporation of poly(glutamic acid) (PAG) and evaporation of a mixture of poly(glutamic acid)/glutaraldehyde (PAG/GLU). All three films showed good adherence to the electrode surface. The performance of the modified GCE was investigated by cyclic voltammetry and differential pulse voltammetry, and the films were characterized by atomic force microscopy (AFM) and electrochemical impedance spectroscopy (EIS). The three poly(glutamic acid) modified GCEs were tested using the electrochemical oxidation of ascorbic acid and a decrease of the overpotential and the improvement of the oxidation peak current was observed. The PAG modified electrode surfaces gave the best results. AFM morphological images showed a polymeric network film formed by well-defined nanofibres that may undergo extensive swelling in solution, allowing an easier electron transfer and higher oxidation peaks.

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“…Electrochemical impedance spectroscopy (EIS) allows the analysis of the properties of materials and systems by applying them alternate electric signals of different frequencies (voltage or current) and measuring the corresponding electric output signals (current or voltage) [45][46][47][48][49][50][51][52]. These signals can be applied in a broad frequency range, which makes it possible to cover a wide range of time constants.…”

Section: Introductionmentioning

confidence: 99%

A novel synthesis of 3,6-bis(2,3-dihydrothieno[3,4-b][1,4]dioxin-5-yl)-9-tosyl- 9H-carbazole

Ateş¹,

Uludag²,

Karazehir³

et al. 2012

Analele Universitatii "Ovidius" Constanta - Seria Chimie

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A novel synthesis of 3,6-bis (2,3-dihydrothieno[3,4-b] (2,3-dihydrothieno [3,4-b][1,4]dioxin-5-yl)-9-tosyl-9H-carbazole (EDOTTsCz) was synthesized and electrochemically polymerized on glassy carbon electrode (GCE) in 0.1 M tetrabutyl ammonium tetrafluoroborate (NB 4 BF 4 )/acetonitrile (CH 3 CN). Alternating copolymer was formed by CV method. Modified polymer electrode was characterized by Fourier transform Infrared Spectroscopy-Attenuated Transmission Reflectance (FTIR-ATR), Cyclic voltammetry (CV), Scanning electron microscopy-Energy dispersive X-ray analysis (SEM-EDX), Atomic force microscope (AFM) and Electrochemical impedance spectroscopy (EIS). Capacitive behaviors of the modified GCE were defined via Nyquist, Bode-magnitude, Bode-phase and Capacitance plots. A modified copolymer electrode provides enhanced capacitance evaluation, which may results in performance in energy storage devices.

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Immobilization of rhodopsin on a self-assembled multilayer and its specific detection by electrochemical impedance spectroscopy

Cited by 102 publications

References 35 publications

A network model to correlate conformational change and the impedance spectrum of single proteins

A network model to correlate conformational change and the impedance spectrum of single proteins

Poly(glutamic acid) nanofibre modified glassy carbon electrode: Characterization by atomic force microscopy, voltammetry and electrochemical impedance

A novel synthesis of 3,6-bis(2,3-dihydrothieno[3,4-b][1,4]dioxin-5-yl)-9-tosyl- 9H-carbazole

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