Immobilization of Rhodococcus opacus 1CP azoreductase to obtain azo dye degrading biocatalysts operative at acidic pH

Qi, Jingxian; Anke, Marlene Kristin; Szymańska, Katarzyna; Tischler, Dirk

doi:10.1016/j.ibiod.2017.01.027

Cited by 25 publications

(7 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Based on flavin cofactor content and nicotinamide dependence or preference, azoreductase superfamily can be classified into five major groups-(1) flavin-containing NADH-dependent azoreductase (Matsumoto et al 2010;Liu et al 2007), (2) flavin-containing NADPH-dependent azoreductase (Chen et al 2005;Wang et al 2007), (3) flavin-containing NAD(P)H-dependent azoreductase (Qi et al 2016(Qi et al , 2017aOturkar et al 2013), (4) flavinfree NAD(P)H-dependent azoreductase (Blumel et al 2002;Chen et al 2010;Misal et al 2011Misal et al , 2014Misal et al , 2015, and (5) flavin-containing NAD(P)H-dependent quinone Fig. 1 Azoreduction mechanism of amaranth dye by azoreductase enzyme: The UV-Visible spectrum demonstrating the complete reduction of amaranth dye in 24 h of incubation with purified azoreductase.…”

Section: Azoreductase Classificationmentioning

confidence: 99%

Azoreductase: a key player of xenobiotic metabolism

Misal

Gawai

2018

Bioresour. Bioprocess.

135

View full text Add to dashboard Cite

Azoreductases are diverse flavoenzymes widely present among microorganisms and higher eukaryotes. They are mainly involved in the biotransformation and detoxification of azo dyes, nitro-aromatic, and azoic drugs. Reduction of azo bond and reductive activation of pro-drugs at initial level is a crucial stage in degradation and detoxification mechanisms. Using azoreductase-based microbial enzyme systems that are biologically accepted and ecofriendly demonstrated complete degradation of azo dyes. Azoreductases are flavin-containing or flavin-free group of enzymes, utilizing the nicotinamide adenine dinucleotide or nicotinamide adenine dinucleotide phosphate as a reducing equivalent. Azoreductases from anaerobic microorganisms are highly oxygen sensitive, while azoreductases from aerobic microorganisms are usually oxygen insensitive. They have variable pH, temperature stability, and wide substrate specificity. Azo dyes, nitro-aromatic compounds, and quinones are the known substrates of azoreductase. The present review gives an overview of recent developments in the known azoreductase enzymes from different microorganisms, its novel classification scheme, significant characteristics, and their plausible degradation mechanisms.

show abstract

Section: Azoreductase Classificationmentioning

confidence: 99%

Azoreductase: a key player of xenobiotic metabolism

Misal

Gawai

2018

Bioresour. Bioprocess.

135

View full text Add to dashboard Cite

show abstract

“…Furthermore, the enzyme was not deactivated via CAPC treatment, indicating that the enzyme-loaded disk cartridge was successfully created through CAPC. This unique flow reactor system based on the CAPC technique enables the efficient continuous decomposition reaction of azo dye and effective regulation of enzymatic activity by means of accurate control of the flow rate, leading the resolution of important issues such as continuous and scale-up operations in a batch-wise experiment [16]. In order to confirm that the mesoporous silica is encapsulated, the results of product using mesoporous silica colored with methylene blue are shown in Figu ure 5 shows a cross section cut with scissors and photographed with an optica scope.…”

Section: Resultsmentioning

confidence: 99%

Fabrication of Enzyme-Loaded Cartridges Using CO2-Assisted Polymer Compression

Aizawa

Matsuura

2021

Technologies

View full text Add to dashboard Cite

An enzyme-loaded disk-shaped cartridge was fabricated using CO2-assisted polymer compression (CAPC), which is a polymer-bonding method that does not use heat. In this process, after the enzyme was loaded onto mesoporous silica, it was placed in a container fabricated from laminated fibrous sheets using CAPC. Subsequently, the lid and container were bonded via CAPC. Verification using the reductive decomposition reaction of an azo dye (methyl red) showed that the enzyme was not deactivated and that a reaction cartridge was created successfully.

show abstract

“…With Rhodococcus enzymes, the same methods plus covalent binding and cross-linked aggregation are currently used for their immobilization. For example, the azoreductase AzoRo from Rhodococcus opacus strain 1CP was covalently immobilized onto the functionalized mesoporous silica, and this method was more successful than cross-linking [103].…”

Section: Choice Of the Immobilization Methodsmentioning

confidence: 99%

“…The only example of enzymatic Rhodococcus biocatalyst being a covalently immobilized azoreductase AzoRo from R. opacus 1CP is shown. The biocatalyst had high azo dye-degrading activity (38 U/mg) and stability (60 h operation) under unfavorable (pH 4) conditions but required supplementation with NADH, FMN, and formate dehydrogenase [103]. Batch Complete degradation of phenol (0.5 g/L) in 5 days; ≥ 5 cycles of re-using; stable for 12 months; stored at +4 • C for 6 months.…”

Section: Environmental Applications Of Rhodococcus Biocatalystsmentioning

confidence: 99%

“…Importantly, Rhodococcus biocatalysts are suitable for various bioremediation conditions, such as single introduction (bioaugmentation or batch processes) [38,70,85,93,96,103,136], semicontinuous pollutant supplementation (with repeated and gradual adding of a substrate, up to 40 times in total) [91], and continuous bioreactors [13,67,70,83,85,100,102,130,136]. Different-type bioreactors are used that are equipped with diverse constructions, from slurry and single-column fluidized bed bioreactors to three-phase airlift and 2-5-column ones.…”

Section: Treatment Of Wastewater Containing Chlorophenolsmentioning

confidence: 99%

See 1 more Smart Citation

Advanced Rhodococcus Biocatalysts for Environmental Biotechnologies

2019

View full text Add to dashboard Cite

The review is devoted to biocatalysts based on actinobacteria of the genus Rhodococcus, which are promising for environmental biotechnologies. In the review, biotechnological advantages of Rhodococcus bacteria are evaluated, approaches used to develop robust and efficient biocatalysts are discussed, and their relevant applications are given. We focus on Rhodococcus cell immobilization in detail (methods of immobilization, criteria for strains and carriers, and optimization of process parameters) as the most efficient approach for stabilizing biocatalysts. It is shown that advanced Rhodococcus biocatalysts with improved working characteristics, enhanced stress tolerance, high catalytic activities, human and environment friendly, and commercially viable are developed, which are suitable for wastewater treatment, bioremediation, and biofuel production.

show abstract

Immobilization of Rhodococcus opacus 1CP azoreductase to obtain azo dye degrading biocatalysts operative at acidic pH

Cited by 25 publications

References 27 publications

Azoreductase: a key player of xenobiotic metabolism

Azoreductase: a key player of xenobiotic metabolism

Fabrication of Enzyme-Loaded Cartridges Using CO2-Assisted Polymer Compression

Advanced Rhodococcus Biocatalysts for Environmental Biotechnologies

Contact Info

Product

Resources

About