Immobilization of Recombinant Glucose Isomerase for Efficient Production of High Fructose Corn Syrup

Abstract

Glucose isomerase is the important enzyme for the production of high fructose corn syrup (HFCS). One-step production of HFCS containing more than 55% fructose (HFCS-55) is receiving much attention for its industrial applications. In this work, the Escherichia coli harboring glucose isomerase mutant TEGI-W139F/V186T was immobilized for efficient production of HFCS-55. The immobilization conditions were optimized, and the maximum enzyme activity recovery of 92% was obtained. The immobilized glucose isomerase showed higher pH, temperature, and operational stabilities with a K value of 272 mM and maximum reaction rate of 23.8 mM min. The fructose concentration still retained above 55% after the immobilized glucose isomerase was reused for 10 cycles, and more than 85% of its initial activity was reserved even after 15 recycles of usage at temperature of 90 °C. The results highlighted the immobilized glucose isomerase as a potential biocatalyst for HFCS-55 production.

A Novel Glucose Isomerase fromCaldicellulosiruptor besciiwith Great Potentials in the Production of High-Fructose Corn Syrup

“…In these reports, most GIs did not achieve a conversion of 55%, some enzymes achieved a conversion of 55% at low-glucose concentrations, and there was no description of the relationship between conversion and substrate concentration. The results of this study showed that the conversion rate of CbGI increased with the increase of substrate concentration, which is somewhat different from previous reports, such as the conversion rate of GI from Thermus oshimai increased first and then decreased [22]. This indicates that CbGI has strong substrate tolerance.…”

Immobilization of the glucose isomerase from Caldicoprobacter algeriensis on Sepabeads EC-HA and its efficient application in continuous High Fructose Syrup production using packed bed reactor

“…This result unveiled an increment shelf life thanks to the immobilization of GICA. Similar trend was also observed in previous study (Jin, et al, 2017).…”

“…Similar results about improving thermostability of immobilized GIs using various supports have been reported in literature (Araya, Urrutia, Romero, Illanes, & Wilson, 2019;Jia, et al, 2018;Jin, et al, 2017;Tükel & Alagöz, 2008). As example, Chen et al, described that the residual activities for free and immobilized GI from Streptomyces rubiginosus onto Eupergit C 250L were 97 and 84%, respectively after 18 h incubation at 60 °C (Tükel & Alagöz, 2008).…”

“…This indicates that adsorbed GICA is more resistant towards pH changes. Improvement of pH stability for immobilized GI was also observed in various study (Jin, et al, 2017;Kamal, Hegazy, Sharada, Abd elhalim, Lotfy, & Mohamed, 2014;Tumturk, Demirel, Altinok, Aksoy, & Hasirci, 2008;Zhao, Cui, Shah, Xu, & Wang, 2016).…”

“…Hence, an extensive number of immobilization methods have been previously reported for GI's (DiCosimo, McAuliffe, Poulose, & Bohlmann, 2013). Nevertheless, the majority of them have some deficiencies like low operational stability (Jin, Xu, Liu, Jia, Liao, Chen, et al, 2017) or the requirement of toxic substances like glutaraldehyde that could cause several environmental hazards during the scale up of the process. Hence, the development of a straightforward and eco-friendly strategy for GI immobilization is still required to comply with the economic requirements for large-scale.…”