Immobilization of recombinant E. coli thermostable lipase by entrapment inside silica xerogel and nanocarbon-in-silica composites

Коваленко, Г. А.; Беклемишев, А. Б.; Perminova, L. V.; Мамаев, А. Л.; Рудина, Н. А.; Мосеенков, С. И.; Кузнецов, Владимир Л.

doi:10.1016/j.molcatb.2013.09.022

Cited by 25 publications

(17 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The lowest specific activity of lipase was observed with the crust type adsorbent. The adverse effect of the carbon surface on the activity of adsorbed lipase was also reported in our previous works [17,29]. It was hypothesized in those works that, in lipase adsorption on hydrophobic carbon, there can be a "wrong" orientation of lipase on the surface.…”

Section: Preparation Of Lipase Active Biocatalystssupporting

confidence: 71%

Synthesis of nanostructured carbon on Ni catalysts supported on mesoporous silica, preparation of carbon-containing adsorbents, and preparation and study of lipase-active biocatalysts

et al. 2016

View full text Add to dashboard Cite

This work continues a series of our studies on the synthesis of nanostructured carbon (NSC) by the pyrolysis of H 2 + C 3 -C 4 alkane mixtures on nickel and cobalt metal catalysts supported on chemically diverse inorganic materials (aluminosilicates, alumina, carbon) having different textural characteristics (mesoporous and macroporous supports) and shapes (granules, foamed materials, and honeycomb mono liths). Here, we consider Ni catalysts supported on granular mesoporous silica (SiO 2 ). It has been elucidated how the yield of synthesized carbon depends on the Ni/SiO 2 catalyst preparation method (homogeneous pre cipitation or impregnation) and on the composition of the impregnating solution, including the molar ratio of its components-nickel nitrate and urea. The morphology of catalytic NSC and Ni distribution in the sil ica granule have been investigated using a scanning electron microscope with an EDX analyzer. Carbon con taining composite supports (NSC/SiO 2 ) have been employed as adsorbents for immobilizing microbial lipase. The enzymatic activity and stability of the resulting biocatalysts have been estimated in transesterifi cation reactions of vegetable (sunflower and linseed) oils involving methyl or ethyl acetate, esterification, and synthesis of capric acid-isoamyl alcohol esters in nonaqueous media.

show abstract

Section: Preparation Of Lipase Active Biocatalystssupporting

confidence: 71%

Synthesis of nanostructured carbon on Ni catalysts supported on mesoporous silica, preparation of carbon-containing adsorbents, and preparation and study of lipase-active biocatalysts

et al. 2016

View full text Add to dashboard Cite

show abstract

“…These cells display enzymatic activity and are uniformly distributed throughout the inorganic matrix as nanoscale inclu sions of the second phase that impart desired proper ties to the synthesized material [46]. As demonstrated by this work, SiO 2 xerogel can be provided with addi tional components that play specific functional roles in biocatalysts: (1) activators and/or stabilizers for enzymes, (2) water retaining agents, (3) adsorbents, and (4) nanostructured carbon [47]. The optimum composition of multicomponent biocatalysts was selected under conditions where (1) the biocatalytic activity was maximum for interesterification reactions in anhydrous medium (containing approximately 0.1-1% H 2 O) and (2) the mechanical strength of granules was high, ensuring stable operation of biocat alysts in the reaction medium.…”

Section: Resultsmentioning

confidence: 99%

Heterogeneous biocatalytic processes of vegetable oil interesterification to biodiesel

et al. 2015

View full text Add to dashboard Cite

The heterogeneous biocatalytic interesterification of vegetable oils to ethyl esters of fatty acids is studied. Interesterification with ethanol or ethyl acetate is performed using biocatalysts obtained by incorpo rating cell lysates of the rE. coli/lip recombinant strain that produces thermostable Thermomyces lanuginosus lipase, into silica xerogel. The interesterification of vegetable oils is performed in both a batch stirred tank reactor and a continuous reactor with a fixed bed of the prepared biocatalyst. It is shown that ethyl acetate is the best acylating reagent for the interesterification of vegetable oil triglycerides to ethyl esters of fatty acids. Unlike ethanol, ethyl acetate does not inactivate the biocatalyst irreversibly. Under the investigated condi tions, the biocatalyst's half inactivation time was 720 hours at 40°C.Keywords: heterogeneous biocatalysts, lysates of a recombinant strain producing thermostable lipase, incor poration into silica xerogel, interesterification of vegetable oils with ethyl acetate

show abstract

“…For example, the properties of GI-active biocatalysts depended slightly upon the presence of included nanocarbons because of weak adhesion of A. nicotianae cells as mentioned above. The activity of LIP-active whole-cells biocatalysts in hydrolysis of triglyceride was twice as high when the CNTs were included [16]. On the other hand, in interesterification the activity of nanocarbons-free (without included CNTs) biocatalysts was maximal [17].…”

Section: Selection Of the Optimal Compositions Of The Biocatalystsmentioning

confidence: 99%

Heterogeneous Biocatalysts Prepared by Immuring Enzymatic Active Components inside Silica Xerogel and Nanocarbons-In-Silica Composites

et al. 2018

View full text Add to dashboard Cite

Proprietary results on preparation and studies of whole-cell and lysates-based heterogeneous biocatalysts with different enzymatic activity were reviewed. A peculiar method was developed for preparing these biocatalysts by immuring (entrapping) enzymatic active components (EAC) inside silica (SiO 2) xerogel and nanocarbons-in-silica composites. Properties of the multi-component composite biocatalysts such as enzymatic activity and operational stability were compared. The effect of the inclusion of nanocarbons such as nanotubes, nanofibers, and onion-like nanospheres with various texture, nanostructure and dispersion were thoroughly studied. With invertase-active biocatalysts, the direct correlation between an increase in the enzymatic activity of the nanocarbons-in-silica biocatalyst and efficiency of EAC adhesion on nanocarbons was observed. The steady-state invertase activity of the baker yeast lysates-based biocatalysts was determined to increase by a factor of 5-6 after inclusion of the multi-walled carbon nanotubes inside SiO 2-xerogel. With lipase-active biocatalysts, the effect of the included nanocarbons on the biocatalytic properties depended significantly on the reaction type. In interesterification of oil-fat blends, the biocatalysts without any included nanocarbons demonstrated the maximal lipase activity. In esterification of fatty acids with aliphatic alcohols, the activity of the biocatalysts increased by a factor of 1.5-2 after inclusion of the aggregated multi-walled carbon nanotubes (CNTs) inside SiO 2-xerogel. In the low-temperature synthesis of isopentyl esters of butyric (C4:0), capric (C10:0), and srearic (C18:0) fatty acids, the lipase-active composite CNTs-in-silica biocatalysts operated without loss of activity for more than thousand hours.

show abstract

Immobilization of recombinant E. coli thermostable lipase by entrapment inside silica xerogel and nanocarbon-in-silica composites

Cited by 25 publications

References 28 publications

Synthesis of nanostructured carbon on Ni catalysts supported on mesoporous silica, preparation of carbon-containing adsorbents, and preparation and study of lipase-active biocatalysts

Synthesis of nanostructured carbon on Ni catalysts supported on mesoporous silica, preparation of carbon-containing adsorbents, and preparation and study of lipase-active biocatalysts

Heterogeneous biocatalytic processes of vegetable oil interesterification to biodiesel

Heterogeneous Biocatalysts Prepared by Immuring Enzymatic Active Components inside Silica Xerogel and Nanocarbons-In-Silica Composites

Contact Info

Product

Resources

About