Immobilization of Protein Molecules by Size‐Selected Metal Clusters on Surfaces

Abstract

Protein immobilization by nanocluster films is a new route to stabilize individual protein molecules and complexes for single‐molecule measurements such as liquid‐phase atomic force microscopy (AFM) imaging (see Figure). The sub‐monolayer films of size‐selected Au clusters present protein binding sites of arbitrary density.

Spatial Distance Effect of Bienzymes on the Efficiency of Sequential Reactions in a Microfluidic Reactor Packed with Enzyme-immobilized Microbeads

“…Only ~50% of the biotin-binding sites on the avidin-coated beads were available for each of the enzymes. These results suggest that by considering the protein foot areas of GOx (50 nm 2 ) 26 and HRP (20 nm 2 ) 27 to be comparable to that of avidin (34 nm 2 ), 21 only one out of two biotin-binding sites of an avidin molecule (note that we assumed only two biotin-binding sites are present for one avidin molecule sitting on a bead surface, because two other binding sites are oriented toward the bead surface) is available for a biotin-GOx or biotin-HRP enzyme molecule. Thus, only one molecule of biotin-GOx or biotin-HRP can be bound to one avidin molecule.…”

Useful multivariate kinetic analysis: Size determination based on cystein-induced aggregation of gold nanoparticles

“…Different studies showed that the change in the localized surface plasmon band is related to the aggregated size of Au NPs [19][20][21]. Typically the Au NPs can be readily modified with thiolcontaining biomolecules because the thiol group exhibits a strong interaction on the metal surface [22]. Generally, modified Au NPs aggregate when the terminal amino group of a biomolecule forms hydrogen bonding with the carboxyl group of another biomolecules on an adjacent nanoparticle [23,24].…”

Modeling the pinning of Au and Ni clusters on graphite

“…For example, gold clusters form sites at which proteins or other organic molecules can be immobilized, 1,2 leading to the intriguing possibility of nanoscale biosensors. If the nanoclusters are projected towards the surface with a certain energy, then different energy deposition regimes result in different interaction processes between the cluster and the substrate.…”

“…These materials have been the subject of experimental investigation via the deposition of such size-selected clusters on surfaces experimentally. [2][3][4]6,7,9,10 Recently a wide ranging experimental study of the pinning thresholds for gold and nickel clusters has been made for N ranging from 10 to 300. Here we investigate the deposition of size-selected Au and Ni clusters using molecular dynamics computer simulations.…”