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Background. The probiotics immobilization technology is one of the most effective ways for controlled and continuous delivery of viable cells into the intestine. It is well known that multifaceted physiological roles of bifidobacteria are to normalize and stabilize the microbiocenosis, to form intestine colonization resistance, to synthesis amino acids, proteins and vitamins, to maintain non-specific resistance of the organism and so all. Such a wide range of positive effects on the macroorganism allows us to consider bifidobacteria as a basis for functional immobilized healthcare products development. Objective. Taxonomic position determination of the Bifidobacterium longum strain selected for immobilization, study of the viability of this bifidobacteria strain in a complex probionic product based on highly dispersed silica in simulated gastrointestinal tract's conditions and after freeze-drying. Methods. The production strain Bifidobacterium longum IMV B-7165 from the Institute of Food Resources of the National Academy of Agrarian Sciences of Ukraine collection of industrial strains has been used in the study. It was isolated from the healthy human infant's gastrointestinal tract. Commonly used bioinformatics, microbiological, biotechnological and statistical methods have been applied. Results. The best alignments for the sequence of bifidobacteria isolate "4202" 16S rRNA (it was previously deposited as Bifidobacterium longum IMV B-7165) and classic dendrograms based on these results were performed. According to the results of microscopic studies of samples of microorganisms with highly dispersed silica products ("Enterosgel", "Sillard P" and "Toxin.Net") it was found that the immobilization of the Streptococcus thermophilus and bifidobacteria cultures did not differ fundamentally. To study the immobilization effect on the bifidobacteria preservation and properties the following carriers were used: "Enterosgel", "Toxin.NET" and "Sillard P". The survival of immobilized bifidobacteria was further studied in simulated gastrointestinal conditions: immobilized cells are better protected from acid and bile, although with increasing acidity, survival decreases in both control and immobilized cells. Conclusions. The taxonomic position of a bifidobacterial isolate from the healthy human infants used in immobilization studies was clarificated (Bifidobacterium animalis subsp lactis). Under the simulated conditions of the upper gastrointestinal tract in the case of acid and bile impact, the best survival was demonstrated by immobilized cultures of bifidobacteria together with the Enterosgel sorbent (a content of 10% by weight of the culture). The survival of immobilized preparations after freeze-drying was slightly reduced in the case of immobilization on the "Enterosgel" and "Toxin.NET" samples of enterosorbents (a content from 15% to 25% by weight of the culture). The best results were observed in the case of immobilization of bifidobacteria with 5% content of the "Toxin.NET" enterosorbent (enterosgel + inulin).
Background. The probiotics immobilization technology is one of the most effective ways for controlled and continuous delivery of viable cells into the intestine. It is well known that multifaceted physiological roles of bifidobacteria are to normalize and stabilize the microbiocenosis, to form intestine colonization resistance, to synthesis amino acids, proteins and vitamins, to maintain non-specific resistance of the organism and so all. Such a wide range of positive effects on the macroorganism allows us to consider bifidobacteria as a basis for functional immobilized healthcare products development. Objective. Taxonomic position determination of the Bifidobacterium longum strain selected for immobilization, study of the viability of this bifidobacteria strain in a complex probionic product based on highly dispersed silica in simulated gastrointestinal tract's conditions and after freeze-drying. Methods. The production strain Bifidobacterium longum IMV B-7165 from the Institute of Food Resources of the National Academy of Agrarian Sciences of Ukraine collection of industrial strains has been used in the study. It was isolated from the healthy human infant's gastrointestinal tract. Commonly used bioinformatics, microbiological, biotechnological and statistical methods have been applied. Results. The best alignments for the sequence of bifidobacteria isolate "4202" 16S rRNA (it was previously deposited as Bifidobacterium longum IMV B-7165) and classic dendrograms based on these results were performed. According to the results of microscopic studies of samples of microorganisms with highly dispersed silica products ("Enterosgel", "Sillard P" and "Toxin.Net") it was found that the immobilization of the Streptococcus thermophilus and bifidobacteria cultures did not differ fundamentally. To study the immobilization effect on the bifidobacteria preservation and properties the following carriers were used: "Enterosgel", "Toxin.NET" and "Sillard P". The survival of immobilized bifidobacteria was further studied in simulated gastrointestinal conditions: immobilized cells are better protected from acid and bile, although with increasing acidity, survival decreases in both control and immobilized cells. Conclusions. The taxonomic position of a bifidobacterial isolate from the healthy human infants used in immobilization studies was clarificated (Bifidobacterium animalis subsp lactis). Under the simulated conditions of the upper gastrointestinal tract in the case of acid and bile impact, the best survival was demonstrated by immobilized cultures of bifidobacteria together with the Enterosgel sorbent (a content of 10% by weight of the culture). The survival of immobilized preparations after freeze-drying was slightly reduced in the case of immobilization on the "Enterosgel" and "Toxin.NET" samples of enterosorbents (a content from 15% to 25% by weight of the culture). The best results were observed in the case of immobilization of bifidobacteria with 5% content of the "Toxin.NET" enterosorbent (enterosgel + inulin).
Subject. Adsorption of probiotic cultures Lactobacillus acidophilus, namely strains 27 and Narine, and Lactobacillus plantarum, on high-dispersed silica. Purpose. The purpose of this study was to investigate the influence of a magnetic field with intensities of 0.03 and 0.09 T and the pH of the medium on the efficiency of cell adsorption of Lactobacillus probiotic strains. Method. Microbiological and biotechnological methods were applied in this research. Hydrogels based on high-dispersed silica "Enterosgel" and "Toxin.NET" were used as adsorbents. Neodymium magnets with intensities of 0.03 T and 0.09 T were employed to create the magnetic field. The efficiency of adsorption was evaluated by the cell survival of probiotic cultures after adsorption on hydrogel preparations under the influence of the magnetic field and in the case of pH adjustment by adding acid or alkali. The functional properties of the obtained composite preparations were assessed by the time of clot formation after milk fermentation and its acidity. Results. The research results demonstrated that immobilized cells of Lactobacillus acidophilus on the hydrogel of methylsilicic acid had enhanced protection not only against harsh conditions of the gastrointestinal tract (low pH and bile influence) but also against magnetic fields with intensities of 0.03 T and 0.09 T, which had an overall negative impact on native (non-immobilized) cells. The functional activity of cells immobilized in this way on the hydrogel based on high-dispersed silica "Enterosgel," evaluated by acidity and clot formation time, was only slightly reduced: up to 4,3-11,3%. This confirms the assumption that the assessment of cell survival by the Koch's cup method does not fully correspond to the real state of the cells. It was also determined that conducting adsorption in the pH range below 4 is impractical, and the optimal conditions for L. plantarum adsorption were in the pH range of 6 to 7. Scope of results. The research findings can be utilized for the development of complex probiotic preparations based on high-dispersed silica, which may be appropriate for preserving the functionality of probiotic cells used for starter cultures or in the production of dietary supplements containing beneficial microorganisms.
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