Immobilization of Platelet-Rich Plasma onto COOH Plasma-Coated PCL Nanofibers Boost Viability and Proliferation of Human Mesenchymal Stem Cells

Abstract

Abstract:The scaffolds made of polycaprolactone (PCL) are actively employed in different areas of biology and medicine, especially in tissue engineering. However, the usage of unmodified PCL is significantly restricted by the hydrophobicity of its surface, due to the fact that its inert surface hinders the adhesion of cells and the cell interactions on PCL surface. In this work, the surface of PCL nanofibers is modified by Ar/CO 2 /C 2 H 4 plasma depositing active COOH groups in the amount of 0.57 at % that were later used for the immobilization of platelet-rich plasma (PRP). The modification of PCL nanofibers significantly enhances the viability and proliferation (by hundred times) of human mesenchymal stem cells, and decreases apoptotic cell death to a normal level. According to X-ray photoelectron spectroscopy (XPS), after immobilization of PRP, up to 10.7 at % of nitrogen was incorporated into the nanofibers surface confirming the grafting of proteins. Active proliferation and sustaining the cell viability on nanofibers with immobilized PRP led to an average number of cells of 258 ± 12.9 and 364 ± 34.5 for nanofibers with ionic and covalent bonding of PRP, respectively. Hence, our new method for the modification of PCL nanofibers with PRP opens new possibilities for its application in tissue engineering.

An injectable platelet lysate-hyaluronic acid hydrogel supports cellular activities and induces chondrogenesis of encapsulated mesenchymal stem cells

“…Platelet lysate, as a protein rich solution, represents a new and exciting opportunity for tissue engineering. PL contains structural constituents of the ECM such as thrombospondin, vitronectin, and fibronectin which enhances cell adhesion and interaction between cells [62]. Combining PL with biomaterials to maintain the beneficial effects of PL over a longer period of time is an approach that received attention more recently [29,37,63].…”

Fabrication and Plasma Modification of Nanofibrous Tissue Engineering Scaffolds

“…Besides CaCO 3 , nanohydroxyapatite (n-HA) has also been coated onto the surface of plasma-activated nanofibrous scaffolds for bone TE purposes. Seyedjafari et al [296] have focused on this particular topic and have examined the capacity of their fabricated PLLA scaffolds for bone formation in vitro using USSCs under osteogenic induction and in vivo after subcutaneous transplantation in mice. In a first step, the authors performed an O 2 plasma treatment for 5 min on electrospun PLLA scaffolds, after which the samples were immersed in a 1% (w/v) solution of n-HA in deionized water.…”

“…Adhesion of MSCs on the surface of untreated PCL (A), COOH-coated PCL (B), COOHcoated PCL with physically adsorbed PRP (C) and COOH-coated PCL with covalently immobilized PRP (D). All images were taken with a magnification of 40× and the scale bar corresponds to 50 µmreproduced from [294] and [296]. Copyright Wiley, 2007. Cell proliferation and viability studies further revealed that the PRP-coated samples exhibited superior levels of cell proliferation and cell viability.…”

“…Copyright Wiley, 2007. Cell proliferation and viability studies further revealed that the PRP-coated samples exhibited superior levels of cell proliferation and cell viability. These enhanced cellular responses can be described by the presence of a high amount of PRP fibronectin at the PCL scaffold surface, which is known to strongly enhance cell adhesion, migration, and proliferation [296].…”

Synthesis, Self-Assembly, and Drug-Release Properties of New Amphipathic Liquid Crystal Polycarbonates

“…Among them, four class of polyesters, namely polyglycolide (PGA), poly(1,3-trimethylene carbonate) (PTMC), polylactide (PLA), poly(caprolactone) (PCL), and their copolymers, are the most widely studied and utilized in biomedical fields. However, exploration of their applications has been hampered by several limitations owing to their specific properties, for example, the PGA degradation rate is too fast with respect to the required application time of materials [ 3 , 4 , 5 ]; and PLA can produce acid degradation products which may cause aseptic inflammation [ 6 ]. Although there is no acid degradation product during the degradation of PTMC [ 7 ], it lacks functional groups to link bioactive primitives.…”