Immobilization of plant peroxidases in cellulose–ionic liquid films

Applications of Ionic Liquids for the Development of Optical Chemical Sensors and Biosensors

“…The cellulose films reconstituted from imidazolium ILs were also explored as a platform for noncovalent and covalent immobilization of several enzymes. 38,88,[104][105][106] The procedures for producing enzyme-containing films differed from each other by the cellulose content, conditions of its dissolution in ILs, and the features of adding biocatalysts into the matrix. Laccase from Rhus vernicifera was the first enzyme, immobilized into cellulose films.…”

“…88 Such films differed advantageously from the cellulose films with the noncovalently immobilized laccase 38 in 1.7 times higher residual catalytic activity and, as a result, higher rate of the substrate transformation (the indicator reactions proceeded within 1 -2 min rather than 12 h or more). This finding seemed to be an indicator of the "friendliness" of [BMIm]Cl with respect to the plant peroxidases.…”

“…Moreover, since the time of dissolution of plant peroxidases in the solution cellulose-[BMIm]Cl increased with an increase in pI values of the enzyme, the immobilization of cationic horseradish peroxidase into cellulose films seemed to be preferable compared to the immobilization of anionic soybean and peanut peroxidases. 88 The cellulose-supported peroxidase sensors were successfully tested in the reactions of aryldiamines (3,3′,5,5′-tetramethylbenzidine, o-dianisidine, and o-phenylenediamine) and catecholamines (dopamine, adrenaline, α-methyldopa, and dobutamine) oxidation by H2O2. The performed tests showed that the substrate specificity of the plant peroxidases, immobilized into the cellulose films and native enzymes, was the same.…”

“…Conditions of noncovalently and covalently immobilizing an enzyme into/on the cellulose films are under detailed investigation. Although there have been some attempts to determine the enzyme substrates (syringaldazine, 38,104,105 guaiacol, 70 aryldiamines, 88 catecholamines, 88 and o-nitrophenyl-β-D-galactopyranoside), 106 the practical use of such kinds of biosensing materials seems to be a future goal. Currently, there exists only one cellulosebased film sensor with a noncovalently immobilized catalytic indicator system of pyronin B-Mn(II)-sodium dodecyl sulfate for fluorescent determination of artemisinin in pharmaceuticals.…”

“…(1) Fabrication of various sensing materials in the different forms (gels, hydrogels, films, and membranes) due to the unique solvent ability of ILs with respect to different matrix materials (TEOS, 70,84 cellulose, [85][86][87][88][89] EC, PMMA, and PVC [74][75][76][77][78][79][80] ), recognition elements (ligands, [77][78][79]85,86,103 indicators, 81,93,94 chromo-responsible dyes, 89 complexes, [74][75][76]80,84,87,90,91 and enzymes 70,88 ), and gaseous analytes (in particular, CO2 82 ).…”