Immobilization of phytase on zeolite modified with iron(II) for use in the animal feed and food industry sectors

Lopes, Marina Momesso; Coutinho, Thamara Carvalho; Malafatti, João Otávio Donizette; Paris, E. C.; Sousa, Cristina Paiva de; Farinas, Cristiane S.

doi:10.1016/j.procbio.2020.10.017

Cited by 20 publications

(9 citation statements)

References 56 publications

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“…Both free and immobilized phytase were incubated for 1 h at pH 2 and 3. Free phytase retains its activity on both pH values but immobilized phytase lost its 40% activity in these acidic conditions 26 . Likewise, Coutinho et al 172 analysed the impact of free and immobilized phytase by incubating them at pH 2, 2.5 and 3 for 1 h followed by 1 h incubation at pH 7.…”

Section: Immobilized Phytases In Fish Feedmentioning

confidence: 99%

“…On a commercial scale, microbes can be easily propagated, and the phytase produce by them is stable and usable under different aquaculture conditions 25 . Over the last decades, considerable progress has been made to understand the effect of microbial phytases as feed additive and their impact on fish growth performance 23,26,27 . The role of phytases in improving the growth performances and nutrition in aquaculture has been documented earlier 23,28–30 .…”

Section: Introductionmentioning

confidence: 99%

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Role of microbial phytases in improving fish health

Priya

Virmani

Pragya

et al. 2023

Reviews in Aquaculture

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Fishmeal is the most preferred protein source in aquafeed industry due to its balanced amino acid and fatty acid profile, but its limited global supply and high price value, necessitates researchers to focus on sustainable alternative sources of protein and plant‐based feed, to meet the growing demand of fish feed. Phytate is a reservoir of phosphorus present in the plants; however, its chelating ability makes it anti‐nutritional. Phytate forms complexes with minerals, proteins, lipids and carbohydrates, confining their accessibility in fish feeds, hence, additional dietary nutrients are required in the fish feed to overcome nutritional deficiencies. The elimination of phytate‐phosphorus through animal faeces and its pass on to aquatic bodies leads to enrichment of nutrients, eutrophication and algal blooms. Microbial phytase segregates phosphorus, minerals, proteins and lower inositol phosphates from phytate. The supplementation of fish feed with microbial phytase enhances the digestibility of minerals, due to which less nutrients are excreted in the faeces; therefore, minimizing pollution in water bodies. Monogastric and agastric animals inherently exhibit the deficiency of phytase; hence, supplementation of dietary material with phytase has been an emerging choice to make dietary nutrients available for fish. Moreover, microbial phytases have a broad pH range and are thermostable, which makes them an excellent choice for commercial fish feed production. Additionally, microbial phytase improves fish growth as these enzymes enhance nutrients bioavailability and their digestion. This review article presents the application of microbial phytases as a feed additive, and their role in enhancing fish growth.

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Section: Immobilized Phytases In Fish Feedmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Role of microbial phytases in improving fish health

Priya

Virmani

Pragya

et al. 2023

Reviews in Aquaculture

View full text Add to dashboard Cite

show abstract

“…The immobilization process can modify the stability of the enzyme at different pH, due to the interactions between the enzyme and the support. In addition, media loading can affect the catalytic activity of the immobilized enzyme by causing the pH of its microenvironment to be different from the pH of the reaction medium, either favoring or hindering catalysis [35]. Hence, the immobilization had a positive effect on the enzyme activity, depending on the pH at which activity was measured.…”

Section: Effect Of Temperature and Ph On Enzyme Stabilitymentioning

confidence: 99%

Improvement in the Thermostability of a Recombinant β-Glucosidase Immobilized in Zeolite under Different Conditions

et al. 2022

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β-Glucosidase is part of the cellulases and is responsible for degrading cellobiose into glucose, a compound that can be used to produce biofuels. However, the use of the free enzyme makes the process more expensive. Enzyme immobilization improves catalytic characteristics and supports, such as zeolites, which have physical-chemical characteristics and ion exchange capacity that have a promising application in the biotechnological industry. This research aimed to immobilize by adsorption a recombinant β-glucosidase from Trichoderma reesei, obtained in Escherichia coli BL21 (DE3), in a commercial zeolite. A Box Behnken statistical design was applied to find the optimal immobilization parameters, the stability against pH and temperature was determined, and the immobilized enzyme was characterized by SEM. The highest enzymatic activity was determined with 100 mg of zeolite at 35 °C and 175 min. Compared to the free enzyme, the immobilized recombinant β-glucosidase presented greater activity from pH 2 to 4 and greater thermostability. The kinetic parameters were calculated, and a lower KM value was obtained for the immobilized enzyme compared to the free enzyme. The obtained immobilization parameters by a simple adsorption method and the significant operational stability indicate promising applications in different fields.

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“…The use of supports in heterogeneous adsorption methods promotes an adsorbent-accessible recovery and simple separation from the aqueous medium [ 38 , 39 , 40 , 41 ]. Additionally, nanocomposites combine individual characteristics to increase desirable properties [ 42 , 43 , 44 , 45 ].…”

Section: Introductionmentioning

confidence: 99%

Modified Silica Nanoparticles from Rice Husk Supported on Polylactic Acid as Adsorptive Membranes for Dye Removal

et al. 2023

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Industrial effluents and wastewater treatment have been a mainstay of environmental preservation and remediation for the last decade. Silica nanoparticles (SiO2) obtained from rice husk (RH) are an alternative to producing low-cost adsorbent and agriculture waste recovery. One adsorption challenge is facilitating the adsorbate separation and reuse cycle from aqueous medium. Thus, the present work employs SiO2 supported on polylactic acid (PLA) nanofibers obtained by the electrospinning method for Rhodamine B (RhB) dye adsorption. The silica surface was modified with trimethylsilyl chloride (TMCS) to increase affinity towards organic compounds. As a result, the silanized surface of the silica from rice husk (RHSil) promoted an increase in dye adsorption attributed to the hydrophobic properties. The PLA fibers containing 40% SiO2 (w w−1) showed about 85–95% capacity adsorption. The pseudo-first-order kinetic model was demonstrated to be the best model for PLA:SiO2 RHSil nanocomposites, exhibiting a 1.2956 mg g−1 adsorption capacity and 0.01404 min−1 kinetic constant (k1) value. In the reuse assay, PLA:SiO2 membranes preserved their adsorption activity after three consecutive adsorption cycles, with a value superior to 60%. Therefore, PLA:SiO2 nanocomposites from agricultural waste are an alternative to “low-cost/low-end” treatments and can be used in traditional treatment systems to improve dye removal from contaminated waters.

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Immobilization of phytase on zeolite modified with iron(II) for use in the animal feed and food industry sectors

Cited by 20 publications

References 56 publications

Role of microbial phytases in improving fish health

Role of microbial phytases in improving fish health

Improvement in the Thermostability of a Recombinant β-Glucosidase Immobilized in Zeolite under Different Conditions

Modified Silica Nanoparticles from Rice Husk Supported on Polylactic Acid as Adsorptive Membranes for Dye Removal

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