Immobilization of Peroxidase on Functionalized MWCNTs-Buckypaper/Polyvinyl alcohol Nanocomposite Membrane

Jun, Lau Yien; Mubarak, Nabisab Mujawar; Yon, Lau Sie; Bing, Chua Han; Khalid, Mohammad; Jagadish, Priyanka; Abdullah, Ezzat Chan

doi:10.1038/s41598-019-39621-4

Cited by 74 publications

(28 citation statements)

References 77 publications

(65 reference statements)

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“…Immobilization efficiency was improved by increasing the proportion of Fe 3 O 4 Np to around 0.5% and then decreasing. Jun et al [21] and Mohamed et al [22] reported that the peroxidase was immobilized on modified multi-walled carbon nanotubes and amidoximated acrylic polymer with an immobilization efficiency of 81.74% and 70%, respectively. The loss of immobilized-HRP activity when the magnetic Fe 3 O 4 Np concentration was increased was attributed to the multiple attachments points of the enzyme to the polymer film [23].…”

Section: Resultsmentioning

confidence: 99%

Encapsulation of HRP Enzyme onto a Magnetic Fe3O4 Np–PMMA Film via Casting with Sustainable Biocatalytic Activity

2020

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Horseradish peroxidase (HRP) enzyme was effectively encapsulated onto an Fe3O4 nanoparticle–polymethyl methacrylate (PMMA) film via the casting method. The HRP was immobilized on the 0.5% Fe3O4Np–PMMA film and characterized by Fourier transform infrared spectroscopy and field emission scanning electron microscopy. Moreover, the reusability, thermal stability, optimum pH, optimum temperature, the influence of metal ions, and the effects of detergent and organic solvent were investigated. After optimizing the immobilization conditions, the highest efficiency of the immobilized enzyme was 88.4% using 0.5% Fe3O4Np–PMMA. The reusability of the immobilized HRP activity was 78.5% of its initial activity after being repeatedly used for 10 cycles. When comparing the free and immobilized forms of the HRP enzyme, changes in the optimum temperature and optimum pH from 30 to 40 °C and 7.0 to 7.5, respectively, were observed. The Km and Vmax for the immobilized HRP were estimated to be 41 mM, 0.89 U/mL for guaiacol and 5.84 mM, 0.66 U/mL for H2O2, respectively. The high stability of the immobilized HRP enzyme was obtained using metal ions, a high urea concentration, isopropanol, and Triton X-100. In conclusion, the applicability of immobilized HRP involves the removal of phenol in the presence of hydrogen peroxide, therefore, it could be a potential catalyst for the removal of wastewater aromatic pollutants.

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Section: Resultsmentioning

confidence: 99%

Encapsulation of HRP Enzyme onto a Magnetic Fe3O4 Np–PMMA Film via Casting with Sustainable Biocatalytic Activity

2020

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“…The further addition of PVA into BP in 45 BP/PVA reduced the tensile strength to 155.27 GPa and Young's modulus to 4.33 GPa. This effect can be attributed to the irregular distribution of the PVA matrix into BP; besides higher concentration of PVA solution blocks the pores of BP at the surface and stops further penetration into BP 50 . Moreover, the solidification of PVA at various locations on the BP surface and the presence of insoluble hydrogels in the PVA solution further reduced or completely blocked the flow of PVA solution into BP.…”

Section: Characterization Of Pva Infiltrated Graphene-based Bucky Papermentioning

confidence: 99%

Graphene/PVA buckypaper for strain sensing application

Mehmood

Mubarak

Khalid

et al. 2020

Sci Rep

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Strain sensors in the form of buckypaper (BP) infiltrated with various polymers are considered a viable option for strain sensor applications such as structural health monitoring and human motion detection. Graphene has outstanding properties in terms of strength, heat and current conduction, optics, and many more. However, graphene in the form of BP has not been considered earlier for strain sensing applications. In this work, graphene-based BP infiltrated with polyvinyl alcohol (PVA) was synthesized by vacuum filtration technique and polymer intercalation. First, Graphene oxide (GO) was prepared via treatment with sulphuric acid and nitric acid. Whereas, to obtain high-quality BP, GO was sonicated in ethanol for 20 min with sonication intensity of 60%. FTIR studies confirmed the oxygenated groups on the surface of GO while the dispersion characteristics were validated using zeta potential analysis. The nanocomposite was synthesized by varying BP and PVA concentrations. Mechanical and electrical properties were measured using a computerized tensile testing machine, two probe method, and hall effect, respectively. The electrical conducting properties of the nanocomposites decreased with increasing PVA content; likewise, electron mobility also decreased while electrical resistance increased. The optimization study reports the highest mechanical properties such as tensile strength, Young’s Modulus, and elongation at break of 200.55 MPa, 6.59 GPa, and 6.79%, respectively. Finally, electrochemical testing in a strain range of ε ~ 4% also testifies superior strain sensing properties of 60 wt% graphene BP/PVA with a demonstration of repeatability, accuracy, and preciseness for five loading and unloading cycles with a gauge factor of 1.33. Thus, results prove the usefulness of the nanocomposite for commercial and industrial applications.

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“…Otherwise, the activity of IHRP decreased with increased temperature. This could be related with denaturation of enzyme with temperature [22].…”

Section: Immobilization Of Hrp On Tinps-coohmentioning

confidence: 98%

“…Among them, TiO2 is quite special as a support because of its excellent resistance to pH and corrosion, superior mechanical strength, and outstanding antimicrobial performance [10,11]. Previously, HRP has been immobilized on colloidal Au modified ITO glass support [12], colloid/cysteamine-modified gold electrode [13], on Fe3O4/nanotubes composites by APTES [5], cubic mesoporous silicate (SBA-16) [14], modified acrylonitrile copolymer membrane [15], kaolin [16], mesoporous activated carbon [17], wool [18], chitosan crosslinked with cyanuric chloride [6], acrylic polymer activated by cyanuric chloride [19], bacterial cellulose [20], magnetic composite microsphere containing polyethylene glycol [21], multi-walled carbon nanotubes (MWCNTs) buckypaper/polyvinyl alcohol composite membrane [22].…”

Section: Introductionmentioning

confidence: 99%

Optimization of the Immobilization Conditions of Horseradish Peroxidase on TiO2-COOH nanoparticles by Box-Behnken Design

Şahin

2019

Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi

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In this study, TiO2 nanoparticles were prepared and-COOH functionalized with 3-(3,4-dihydroxyphenyl) propionic acid. The characterization of nanoparticles was performed by FTIR, TEM, EDS and XRD. HRP was immobilized on those nanoparticles by EDC/NHS coupling reaction. The immobilization conditions of HRP including A: enzyme concentration (0.5-1.5 mg/mL), B: immobilization pH (4.0-8.0), C: immobilization temperature (4-50°C), D: immobilization time (1-20 h) were optimized by response surface methodology and Box-Behnken design. The optimized immobilization conditions were identified as 0.5 mg/mL HRP, at pH 5.5, 40 °C for 8 h for activity of immobilized HRP, 1.5 mg/mL HRP, at pH 4 and 18°C for 20 h for protein binding yield (%). At these optimum conditions, the experimental value for the activity of immobilized HRP was 80.39 U ± 1.06; protein binding yield was 94.25 ± 3.58%. Moreover, the optimum temperature and pH of free and immobilized enzyme were determined as 50°C and 4.0; 50°C and 3.5, respectively. The activity of the immobilized HRP sustained 52% of its initial activity after 10 days storage at 4°C. Furthermore, the immobilized HRP sustained 48% of its initial activity after 6 consecutive reactions.

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Immobilization of Peroxidase on Functionalized MWCNTs-Buckypaper/Polyvinyl alcohol Nanocomposite Membrane

Cited by 74 publications

References 77 publications

Encapsulation of HRP Enzyme onto a Magnetic Fe3O4 Np–PMMA Film via Casting with Sustainable Biocatalytic Activity

Encapsulation of HRP Enzyme onto a Magnetic Fe3O4 Np–PMMA Film via Casting with Sustainable Biocatalytic Activity

Graphene/PVA buckypaper for strain sensing application

Optimization of the Immobilization Conditions of Horseradish Peroxidase on TiO2-COOH nanoparticles by Box-Behnken Design

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