Immobilization of Pepsin onto Chitosan Silica Nanobeads with Glutaraldehyde as Crosslink Agent

Cahyaningrum, Sari Edi; Sianita, Maria Monica

doi:10.9767/bcrec.9.3.7060.263-269

Cited by 9 publications

(6 citation statements)

References 15 publications

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“…Immobilization of crosslinked chitosan onto both nano-SiO 2 and nano-TiO 2 simultaneously was achieved by using a glutaraldehyde cross-linker . In a 250 mL round-bottomed flask, 2.0 g of the dried crosslinked chitosan, 1.0 g of acid activated nano-SiO 2 , and 1.0 g of acid-activated nano-TiO 2 were combined with 50 mL of glutaraldehyde.…”

Section: Materials and Experimentsmentioning

confidence: 99%

“…Immobilization of crosslinked chitosan onto both nano-SiO 2 and nano-TiO 2 simultaneously was achieved by using a glutaraldehyde crosslinker. 20 In a 250 mL round-bottomed flask, 2.0 g of the dried crosslinked chitosan, 1.0 g of acid activated nano-SiO 2 , and 1.0 g of acidactivated nano-TiO 2 were combined with 50 mL of glutaraldehyde. The mixture was slightly acidified with 5.0 mL of 0.1 M HCl solution and then refluxed for ∼6 h. The product was washed with DDW several times and then dried at 80 °C overnight.…”

Section: ■ Materials and Experimentsmentioning

confidence: 99%

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Imprinting “Nano-SiO₂-Crosslinked Chitosan-Nano-TiO₂” Polymeric Nanocomposite for Selective and Instantaneous Microwave-Assisted Sorption of Hg(II) and Cu(II)

Mahmoud

Nabil

Abdel-Aal

et al. 2018

ACS Sustainable Chem. Eng.

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An imprinting polymeric nanocomposite-like structure was synthesized by bonding crosslinked chitosan to nano-SiO 2 and nano-TiO 2 simultaneously via bifunctional glutaraldehyde linker. The structure of this "nano-SiO 2 -crosslinked chitosan-nano-TiO 2 " nanocomposite was confirmed and characterized by Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD), and thermogravimetric analysis (TGA). The surface area (6.47 cm 3 g −1 ), porosity (0.193 cm 3 g −1 ), and particle size (14−40 nm) were confirmed from scanning electron microscopy (SEM) studies, high-resolution transmission electron microscopy (HR-TEM) studies, and surface area measurements. This nanocomposite announced selective and enhanced sorption for Hg and Cu ions with high performance using microwave techniques. Different factors that affected the metal sorption capacity were monitored, including microwave-contact time, pH, nanocomposite dosage, temperature, and initial metal concentration. Maximum capacity values of 8000 and 4000 μmol g −1 for mercury and copper, respectively, were observed at pH 6 using only 20 s. Standard thermodynamic parameters were also calculated and perfectly referred to spontaneous sorption operations. The sorption of divalent mercury and copper were successfully explained by three isotherm modelsLangmuir, Freundlich, and Dubinin−Radushkevich (D-R)showing favorable operations. The nanocomposite maintained its selectivity and sorption power for mercury and copper, even in the presence of other interfering ions. Microwave-assisted techniques achieved fast effective operations for the extraction of mercury (with 98.0%−99.5%) and copper (with 94.4%−96.6%) from different water samples.

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Section: Materials and Experimentsmentioning

confidence: 99%

Section: ■ Materials and Experimentsmentioning

confidence: 99%

Imprinting “Nano-SiO₂-Crosslinked Chitosan-Nano-TiO₂” Polymeric Nanocomposite for Selective and Instantaneous Microwave-Assisted Sorption of Hg(II) and Cu(II)

Mahmoud

Nabil

Abdel-Aal

et al. 2018

ACS Sustainable Chem. Eng.

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“…In order to fully explain the results of pectinase immobilization, we performed FT-IR comparison between porous spherical rGO and porous spherical rGO immobilized pectinase (FT-IR; Bruker Vector 22, 4000–500 cm –1 ). As shown in Figure , the typical peaks of porous spherical rGO immobilized pectinase were observed at 1654 cm –1 (−CN stretching vibration), 1542 cm –1 (−CO stretching vibrations), and 1140 cm –1 (C–O–C asymmetrical stretching vibration, an acyl transition state). − This fully elucidates the specific amino structure of the enzyme molecule introduced by immobilization.…”

Section: Results and Discussionmentioning

confidence: 66%

Green Synthesis of Porous Spherical Reduced Graphene Oxide and Its Application in Immobilized Pectinase

Gao

et al. 2020

ACS Omega

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Pectinase is widely used in juice production, food processes, and other fields. However, owing to poor stability, free pectinase is difficult to separate from a substrate after hydrolysis and cannot be reused, and thus its industrial use is limited. Immobilized pectinase can solve these problems well. We prepared a carrier material of immobilized enzyme, which is called porous spherical reduced graphene oxide (rGO) with a rich pore structure, large specific surface area, strong hardness, and good biocompatibility to enzyme. Then, we evaluated the performance of the porous spherical rGO immobilized pectinase and characterized its structure by IR, XRD, and SEM. Using this material as a carrier of immobilized enzyme improves the load and catalytic activity of the enzyme. After 10 times of continuous use, the porous spherical rGO immobilized enzyme still maintained its initial relative enzyme activity at around 87%, indicating that immobilized pectinase had a stronger cycling stability, and its thermal stability, acid–base tolerance, and storage stability were superior to those of free pectinase. The results were compared with those of other studies on immobilized pectinase. The relative activity of pectinase immobilized by porous spherical rGO was at a high level after 10 consecutive uses. Overall, the spherical rGO is an excellent immobilized enzyme carrier material.

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“…A good supporting matrix is a matrix that has a large surface area, can be penetrated, stable and resistant to microbial contamination, and is stable to changes in pH, temperature, etc. [9].…”

Section: Introductionmentioning

confidence: 99%

Stability and Esterification Activity of Candida rugosa Lipase Immobilized on Celite with Acetone Solvent

Zamara,

Yudhana,

Curie

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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Candida rugosa lipase (CRL) is an enzyme that is widely used in biopharmaceutical and biopesticide industries due to its high catalytic ability and reusability. However, conditions from industrial processes that are often outside CRL stability range may cause denaturation or destabilization of the enzyme, causing it to be not reusable, hence lowering its economic value. To increase its catalytic activity, stability, and reusability, CRL enzyme can be immobilized in a matrix. In this study, the CRL was physically adsorbed onto Celite-545. The immobilization process was done using buffer solution with the addition of acetone. The influence of initial enzyme concentration and immobilization condition (time, temperature, and pH) were optimized using OFAT method. The data were taken in terms of esterification activity, thermal stability, and protein content. The result showed good thermal stability of the immobilized biocatalysts and an increase in esterification activity at optimum conditions that supported the efficiency of Celite-545 as lipase immobilization supports.

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Immobilization of Pepsin onto Chitosan Silica Nanobeads with Glutaraldehyde as Crosslink Agent

Cited by 9 publications

References 15 publications

Imprinting “Nano-SiO₂-Crosslinked Chitosan-Nano-TiO₂” Polymeric Nanocomposite for Selective and Instantaneous Microwave-Assisted Sorption of Hg(II) and Cu(II)

Imprinting “Nano-SiO₂-Crosslinked Chitosan-Nano-TiO₂” Polymeric Nanocomposite for Selective and Instantaneous Microwave-Assisted Sorption of Hg(II) and Cu(II)

Green Synthesis of Porous Spherical Reduced Graphene Oxide and Its Application in Immobilized Pectinase

Stability and Esterification Activity of Candida rugosa Lipase Immobilized on Celite with Acetone Solvent

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Immobilization of Pepsin onto Chitosan Silica Nanobeads with Glutaraldehyde as Crosslink Agent

Cited by 9 publications

References 15 publications

Imprinting “Nano-SiO2-Crosslinked Chitosan-Nano-TiO2” Polymeric Nanocomposite for Selective and Instantaneous Microwave-Assisted Sorption of Hg(II) and Cu(II)

Imprinting “Nano-SiO2-Crosslinked Chitosan-Nano-TiO2” Polymeric Nanocomposite for Selective and Instantaneous Microwave-Assisted Sorption of Hg(II) and Cu(II)

Green Synthesis of Porous Spherical Reduced Graphene Oxide and Its Application in Immobilized Pectinase

Stability and Esterification Activity of Candida rugosa Lipase Immobilized on Celite with Acetone Solvent

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Product

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Imprinting “Nano-SiO₂-Crosslinked Chitosan-Nano-TiO₂” Polymeric Nanocomposite for Selective and Instantaneous Microwave-Assisted Sorption of Hg(II) and Cu(II)

Imprinting “Nano-SiO₂-Crosslinked Chitosan-Nano-TiO₂” Polymeric Nanocomposite for Selective and Instantaneous Microwave-Assisted Sorption of Hg(II) and Cu(II)