Immobilization of papain enzyme on a hybrid support containing zinc oxide nanoparticles and chitosan for clinical applications

Soares, Aurileide Maria Bispo Frazão; Gonçalves, Lizia M. O.; Ferreira, Ruanna D. S.; Souza, Jefferson M. de; Fangueiro, Raúl; Alves, Michel Muálem de Moraes; Carvalho, Fernando Aécio de Amorim; Mendes, Anderson Nogueira; Cantanhêde, Welter

doi:10.1016/j.carbpol.2020.116498

Cited by 57 publications

(21 citation statements)

References 56 publications

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“…It is possible to extract retinol, α‐tocopherol, ascorbic acid and papain (Enzyme Commission Number: EC 3.4.22.2), an enzyme widely used in medicine to treat intestinal disorders and skin wounds [236] . Papain can be incorporated into chitosan films and combined with zinc oxide to obtain biomaterials with low cytotoxicity to skin [237] . The papaya peel can be pyrolyzed and further modified with EDTA to produce luminescent carbon nanodots with the ability to undergo chromium complexation [237] …”

Section: Fruits and Nuts As Potential Brazilian Renewable Sourcesmentioning

confidence: 99%

“…[236] Papain can be incorporated into chitosan films and combined with zinc oxide to obtain biomaterials with low cytotoxicity to skin. [237] The papaya peel can be pyrolyzed and further modified with EDTA to produce luminescent carbon nanodots with the ability to undergo chromium complexation. [237]…”

Section: Papayamentioning

confidence: 99%

“…[237] The papaya peel can be pyrolyzed and further modified with EDTA to produce luminescent carbon nanodots with the ability to undergo chromium complexation. [237]…”

Section: Papayamentioning

confidence: 99%

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Opportunities for the Use of Brazilian Biomass to Produce Renewable Chemicals and Materials

et al. 2020

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This Review highlights the principal crops of Brazil and how their harvest waste can be used in the chemicals and materials industries. The Review covers various plants; with grains, fruits, trees and nuts all being discussed. Native and adopted plants are included and studies on using these plants as a source of chemicals and materials for industrial applications, polymer synthesis, medicinal use and in chemical research are discussed. The main aim of the Review is to highlight the principal Brazilian agricultural resources; such as sugarcane, oranges and soybean, as well as secondary resources, such as andiroba brazil nut, buriti and others, which should be explored further for scientific and technological applications. Furthermore, vegetable oils, carbohydrates (starch, cellulose, hemicellulose, lignocellulose and pectin), flavones and essential oils are described as well as their potential applications.

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Section: Fruits and Nuts As Potential Brazilian Renewable Sourcesmentioning

confidence: 99%

Section: Papayamentioning

confidence: 99%

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Opportunities for the Use of Brazilian Biomass to Produce Renewable Chemicals and Materials

et al. 2020

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“…The application of colorimetric detection depends on natural enzymes such as peroxidase. Recently, many nanomaterials, such as ZnO nanoparticles [ 3 ], conjugated microporous polymer (CMP-LS9) [ 4 ] and porous carbon nanozymes [ 5 ], have been used in colorimetric detection base on their enzyme-like catalytic ability. Liu et al [ 6 ] used 3,4:9,10-perylene tetracarboxylic acid (PTCA) modified litchi-like zinc ferrite (ZnFe 2 O 4 ) to prepare nanocomposite material, which was used for the colorimetric detection of ascorbic acid.…”

Section: Introductionmentioning

confidence: 99%

Biocompatible Platinum Nanoclusters Prepared Using Bitter Gourd Polysaccharide for Colorimetric Detection of Ascorbic Acid

Zhao

Zhang

et al. 2021

Biomolecules

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Ascorbic acid is an organic compound with antioxidant properties that can protect the human body from the threat of free radicals. Therefore, it is important to detect the existence and measure the concentration of ascorbic acid to regulate its content in the human body. In this work, we prepared bitter gourd polysaccharide (BGP)-stabilized platinum nanoclusters (Pt-BGP NCs) by reacting BGP with K2PtCl4. Pt-BGP NCs and catalyzed the decomposition of H2O2 to generate •OH radicals, which could oxidize TMB to form oxidized TMB (oxTMB), indicating their peroxidase-like properties. The kinetics followed the Michaelis–Menten equation. Furthermore, the colorimetric detection of ascorbic acid using Pt-BGP NCs showed high selectivity and a low detection limit of 0.191 μM. The accuracy of real sample detection using Pt-BGP NCs was as high as 98.9%. More importantly, Pt-BGP NCs had high cell biocompatibility and extremely low hemolysis rate due to the component of BGP. In summary, the prepared Pt-BGP NCs with reductive activity and good biocompatibility have good application prospects in colorimetric detection of ascorbic acid.

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“…Papain is a proteolytic enzyme with one polypeptide with 212 amino acid residues, a molecular mass of 23.4 kDa, isoelectric point at 8.75, and optimal activity at pH 5-8. This enzyme has great potential for the development of active packaging, due to its antibacterial and antioxidant properties (Alpay & Uygun, 2015;Eshamah et al, 2014;Manohar et al, 2015;Soares et al, 2020). In addition, it is commonly used in meat tenderization (Abdel-Naeem & Mohamed, 2016;Barekat & Soltanizadeh, 2017), production of protein hydrolysates (Alpay & Uygun, 2015), clarification of fruit juice, and prevention of turbidity of beers during prolonged cold storage (Esti et al, 2013;Mosafa et al, 2013).…”

mentioning

confidence: 99%

Properties of chitosan–papain biopolymers reinforced with cellulose nanofibers

Santos

Oliveira

Lago

et al. 2021

J. Food Process. Preserv.

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The growing demand for environmentally friendly packaging has led to studies on the development of biodegradable packaging made from biopolymers. Among the natural polymers used for the development of biodegradable packaging, chitosan stands out due to its excellent film-forming capacity and good barrier properties (de Castro e Silva et al., 2020;de Moraes Crizel et al., 2018;de Oliveira et al., 2019). Chitosan is produced industrially by deacetylation of chitin, which is the second most abundant polysaccharide in nature nontoxic, biodegradable, biocompatible, commercially available, with wide application in the production of biodegradable films for food packaging (de

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Immobilization of papain enzyme on a hybrid support containing zinc oxide nanoparticles and chitosan for clinical applications

Cited by 57 publications

References 56 publications

Opportunities for the Use of Brazilian Biomass to Produce Renewable Chemicals and Materials

Opportunities for the Use of Brazilian Biomass to Produce Renewable Chemicals and Materials

Biocompatible Platinum Nanoclusters Prepared Using Bitter Gourd Polysaccharide for Colorimetric Detection of Ascorbic Acid

Properties of chitosan–papain biopolymers reinforced with cellulose nanofibers

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