Immobilization of oxidoreductases on gold and silver nanoparticles

Synenka, Mariya; Stasyuk, Nataliya; Semashko, T. V.; Gayda, Galina; Михайлова, Р. В.; Гончар, М. В.

doi:10.30970/sbi.0803.365

Cited by 3 publications

(3 citation statements)

References 20 publications

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“…As described earlier [42][43], the average diameters (dav) of all monometallic NPs and AuNWs estimated by SEM are about 100 nm. XRM data of these NPs proved the forming of Au 0 and Ag 0 compounds, with characteristic peaks Kα at 2.1 keV and 3.0 keV, respectively.…”

Section: Study Of the Synthesized Particles With Sem And Xrmsupporting

confidence: 63%

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Cell Imaging with Fluorescent Bi-Metallic Nanoparticles

Stasyuk

Gayda

Serkiz

et al. 2015

JAC

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Last decades various imaging techniques have been applied in biological and biomedical research, such as magnetic resonance imaging, different types of tomography, fluorescence/bioluminescence, ultrasound, as well as multimodality approaches. Fluorescence imaging, especially in combination with nanoscale materials, is a very prospective tool for experiments in vivo and clinical applications due to its high temporal and spatial resolutions. Fluorescent n anoparticles (NPs), having ability to interact with biomolecules both on the surface of and inside the cells, may revolutionize the cell imaging approaches for diagnostics and therapy. In our investigation we report about new method of cell imaging with fluorescent bi-metallic NPs synthesized by chemical reduction of the relevant ions. As the model of living organism, the cells of yeast Hansenula polymorpha were used. All NPs in minimal concentration (up to 0.05 mM) was proved to be nontoxic for yeast cells. The NPs and NPs -modified cells were characterized with the methods of UV-VIS spectroscopy, scanning electron microscopy, atom force microscopy, transmission electron m icroscopy and fluorescence microscopy. The bimetallic NPs, possessing the stable fluorescence in solution and inside the cells, allow to observe the phenomenon of NPs transferring from parental to daughter cells through at least three generations followed by releasing from the modified cells. The fluorescent NPs synthesized being small, non-toxic and fluorescent was shown to be perspective tool for cell imaging. KeywordsNanoparticles of noble metals· Fluorescence ·Toxicity of nanoparticles · Yeast Hansenula polymorpha ·Cell imaging .

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Section: Study Of the Synthesized Particles With Sem And Xrmsupporting

confidence: 63%

“…Nanoparticles (NPs) and nanowebs (NWs) were synthesized by the reduction of metal ions from appropriate salts according to the known methods in our modifications [42][43]. Conditions of NPs and NWs synthesis are presented in the Table 1.…”

Section: Synthesis Of Nanoparticlesmentioning

confidence: 99%

Cell Imaging with Fluorescent Bi-Metallic Nanoparticles

Stasyuk

Gayda

Serkiz

et al. 2015

JAC

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“…A commercial lyophilized glucose oxidase (GO, EC 1.1.3.4) from Asperigillus niger (Sigma, St. Louis, MO) with an activity of 100 U•mg −1 was dissolved in 20 mM phosphate buffer, pH 6.0, up to 100 U•mL −1 . GO activity was assayed in a reaction mixture containing 0.16 mM o-dianisidine, 1.61% (w/v) glucose, and 2 U/mL of PO in 50 mM sodium acetate buffer, pH 5.0, as described earlier [35].…”

Section: Enzymesmentioning

confidence: 99%

“Green” Nanozymes: Synthesis, Characterization, and Application in Amperometric (Bio)sensors

Gayda

Demkiv

Gurianov

et al. 2020

The 1st International Electronic Conference on Biosensors

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Nanozymes (NZs) are catalytically active nanomaterials that have enzyme-like activity but possess increased stability and greater availability due to the fact of their simpler preparation technologies. Nanozymes as nanoscale artificial enzymes demonstrate various catalytic specificities as oxidoreductases, such as peroxidase, catalase, laccase, and others as well as hydrolases, proteases, endonucleases, DNA-ases, NO synthases, etc. A broad variety of NZs exhibits dual- or multienzyme mimetic activity. Nanozymes as stable, low-cost mimetics of natural enzymes have a high potential for application in different branches of biotechnology including scientific investigations, industry, and ecology. Nanozymes can be applied in medicine as diagnostic tools and components of therapeutic drugs. Since NZs have high catalytic activity and chemical and biological stability, they are very promising in the construction of biosensors and biofuel cells. For these reasons, the search for simple methods of synthesis and characterization of different NZs is a very important and real problem. The “green” synthesis of Prussian blue analogous as peroxidase-like NZs using oxido-reductases is described in this study. The obtained green-synthesized hexacyanoferrates (gHCFs) of transition metals were characterized by structure, size, composition, catalytic properties, electro-mediator activities, and substrate specificity. Copper hexacyanoferrate (gCuHCF) was studied in more detail. When immobilized on a graphite electrode (GE), gCuHCF under special conditions of pH and tension gave amperometric signals on hydrogen peroxide and can be used as a peroxidase mimetic in oxidase-based biosensors. Under other conditions, gCuHCF/GE reacts to other analytes. We propose that gHCFs of transition metals synthesized via enzymes may become prospect platforms for the construction of multi-functional amperometric (bio)sensors.

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“Green” Prussian Blue Analogues as Peroxidase Mimetics for Amperometric Sensing and Biosensing

et al. 2021

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Prussian blue analogs (PBAs) are well-known artificial enzymes with peroxidase (PO)-like activity. PBAs have a high potential for applications in scientific investigations, industry, ecology and medicine. Being stable and both catalytically and electrochemically active, PBAs are promising in the construction of biosensors and biofuel cells. The “green” synthesis of PO-like PBAs using oxido-reductase flavocytochrome b2 is described in this study. When immobilized on graphite electrodes (GEs), the obtained green-synthesized PBAs or hexacyanoferrates (gHCFs) of transition and noble metals produced amperometric signals in response to H2O2. HCFs of copper, iron, palladium and other metals were synthesized and characterized by structure, size, catalytic properties and electro-mediator activities. The gCuHCF, as the most effective PO mimetic with a flower-like micro/nano superstructure, was used as an H2O2-sensitive platform for the development of a glucose oxidase (GO)-based biosensor. The GO/gCuHCF/GE biosensor exhibited high sensitivity (710 A M−1m−2), a broad linear range and good selectivity when tested on real samples of fruit juices. We propose that the gCuHCF and other gHCFs synthesized via enzymes may be used as artificial POs in amperometric oxidase-based (bio)sensors.

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Immobilization of oxidoreductases on gold and silver nanoparticles

Cited by 3 publications

References 20 publications

Cell Imaging with Fluorescent Bi-Metallic Nanoparticles

Cell Imaging with Fluorescent Bi-Metallic Nanoparticles

“Green” Nanozymes: Synthesis, Characterization, and Application in Amperometric (Bio)sensors

“Green” Prussian Blue Analogues as Peroxidase Mimetics for Amperometric Sensing and Biosensing

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