Immobilization of Oligonucleotides for Biochemical Sensing by Self-Assembled Monolayers: Thiol-Organic Bonding on Gold and Silanization on Silica Surfaces

Luderer, Frank; Walschus, Uwe

doi:10.1007/128_003

Cited by 51 publications

(43 citation statements)

References 60 publications

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“…The physicochemical properties of functionalized gold surface can be tailored by varying the chain length, terminal functional group and chemical composition of capping chains. The molecular assemblies of SAM of thiol on the coinage metal surfaces have potential applications in protective surface coatings, surface chemistry, molecular electronics, lubricants, nanolithography, sensors, bio-materials, medical implants and pharmacology due to their ordered arrangement, controllable properties, bio-compatibility, stability, selectivity and sensibility [3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18]. Alkane thiols form well-packed, stable and ordered SAM on the gold surface due to the spontaneous formation of strong gold sulfur bonds and the van der Waals interactions between the adjacent thiol chains [19,20].…”

Section: Introductionmentioning

confidence: 99%

A simulation study on the thermal and wetting behavior of alkane thiol SAM on gold (111) surface

Devi

2014

Progress in Natural Science: Materials International

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Molecular dynamics simulations have been performed to investigate the structural, thermal and wetting properties of self-assembled monolayer (SAM) of alkane thiol on gold surface. The specific heat capacity of the gold SAM surface was found to linearly increase with the temperature in the range 100-300 K. It was found to drop down at 400 K and this decrease might be attributed to the disorder of the SAM chains. Hydration of gold SAM surface for two different terminal groups, namely methyl (hydrophobic), and hydroxy (hydrophilic) was studied at room temperature. The difference in their wetting behavior and the structure of their interfacial water were examined from the estimation of the z density profile, radial distribution function, hydrogen bonds and orientation of water dipoles in the interfacial region. The present simulation results suggest that the wetting behavior of the gold SAM surface can be modified by altering the terminal functional group of the SAM chains.

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Section: Introductionmentioning

confidence: 99%

A simulation study on the thermal and wetting behavior of alkane thiol SAM on gold (111) surface

Devi

2014

Progress in Natural Science: Materials International

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“…Of the many SAM systems developed, thiol-based [4,5] and silane-based [6,7] systems are the most extensively studied, with the former usually assembled on gold surfaces and the latter on the oxide surfaces of silicon or glass. The properties and applications of both of these SAM systems have been covered in numerous reviews [5,[8][9][10][11][12][13][14][15] and innumerable individual contributions.…”

Section: Introductionmentioning

confidence: 99%

Thermal stability of thiol and silane monolayers: A comparative study

Chandekar

Sengupta

Whitten

2010

Applied Surface Science

117

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“…Silanization of slides with monoalkoxysilanes in the presence of butyltrimethoxysilane (BMS) (1:1) (Pohl et al, 1986;Luderer and Walschus, 2005) was additionally performed at a concentration of 0.2%. After silanization, the slides were washed with toluene and heated at 110EC for 1 hour in order to form a covalent bond be- Fig.…”

Section: Cleaning and Silanization Of Glass Supportsmentioning

confidence: 99%

Research paper Application of epoxy functional silanes in the preparation of DNA microarrays

Frydrych-Tomczak¹,

Uszczyńska²,

Ratajczak³

et al. 2014

bta

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Nucleic acid microarrays have recently become one of the basic techniques in the study of gene expression. Owing to progress in the field of miniaturization, thousands of oligonucleotides differing in terms of their sequences can be systematically placed on a small area of a solid support (usually glass). These probes are capable of simultaneously interacting with a large number of longer nucleic acids from particular genes. Nucleic acid microarray construction technology consists, primarily, of the appropriate functionalization of a glass surface with the use of organofunctional silanes. Oligonucleotides known as probes are attached to a functionalized surface using, for instance, the lithography technique and, after being linked to the surface, they are subjected to hybridization with complementary and labelled fragments of nucleic acids known as samples of unknown sequences. In this paper, we present a method for constructing DNA microarrays that is based on the use of microscopic slides modified with epoxy functional group-containing silanes. This study was aimed at optimizing the production of DNA microarrays. The study tested the usefulness of four different epoxy functional silanes with one or three alkoxy groups. In addition, slides were silanized with the use of alkylsilane. The glass slides were characterized using a goniometer and an atomic force microscope (AFM). The synthesized amino linker-containing oligonucleotide probes were printed onto the glass slides in order to check the effectiveness of their attachment to the solid surface.

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Immobilization of Oligonucleotides for Biochemical Sensing by Self-Assembled Monolayers: Thiol-Organic Bonding on Gold and Silanization on Silica Surfaces

Cited by 51 publications

References 60 publications

A simulation study on the thermal and wetting behavior of alkane thiol SAM on gold (111) surface

A simulation study on the thermal and wetting behavior of alkane thiol SAM on gold (111) surface

Thermal stability of thiol and silane monolayers: A comparative study

Research paper Application of epoxy functional silanes in the preparation of DNA microarrays

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