Immobilization of nattokinase-loaded red blood cells on the surface of superhydrophobic polypropylene targeting fibrinolytic performance

Li, Chunming; Wei, Ye; Jin, Jing; Xu, Xiaodong; Liu, Jingchuan; Yin, Jinghua

doi:10.1039/c5tb00444f

Cited by 13 publications

(8 citation statements)

References 37 publications

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“…On the basis of Erbil’s work, ziPP films were fabricated by solution casting. The water CA of the film was 158.8°, as shown in Figure , which is similar to the reported value of approximately 160°. − However, this film was not truly superhydrophobic due to a high SA (32°). The thermal stability of the ziPP surfaces was further investigated.…”

Section: Resultssupporting

confidence: 85%

Thermally Stable Polypropylene Superhydrophobic Surface Due to the Formation of a Surface Crystalline Layer of Microsized Particles

Zhang

Chen

et al. 2019

J. Phys. Chem. C

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Polypropylene is a potential material for the fabrication of superhydrophobic surfaces. In this paper, two isotactic polypropylenes made from a metallocene catalyst (miPP) and Ziegler–Natta catalyst (ziPP) were employed to prepare superhydrophobic surfaces. The results showed that the superhydrophobicity and thermal stability of the miPP film were much better than those of the ziPP film. The water contact angle (CA) and sliding angle (SA) of the miPP film were 155° and 10°, respectively, after being heated at 100 °C for 5 h, while the water CA and SA of the ziPP film were 135° and 90°, respectively. This difference in the thermal stability was attributed to the miPP easily forming a surface crystalline layer on the microsized particles, which resulted in an increased roughness and improved thermal stability. These results were supported by the dyeing experiment, high-resolution transmission electron microscopy (HRTEM) images, and selected-area electron diffraction patterns. Furthermore, the morphology and roughness of the spin-coated miPP and ziPP films were explored by atomic force microscopy (AFM), and the results also showed that the good thermal stability of miPP film resulted from the increased crystallinity.

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Section: Resultssupporting

confidence: 85%

Thermally Stable Polypropylene Superhydrophobic Surface Due to the Formation of a Surface Crystalline Layer of Microsized Particles

Zhang

Chen

et al. 2019

J. Phys. Chem. C

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“…On the other hand, using the fluorescence intensity changes to monitor the enzyme renewal process in PPMECs is an attractive protocol. 8,34,148 P(AA-ES) was formed by the acrylic acid monomer covalently grafted to the surface of P(ES) via UV irradiation. 34 The carboxylic functional groups of P(ES) were activated by 1ethyl-3−3(dimethylaminopropyl)carbodiimide (EDC) and the activated groups were displaced by a nucleophilic attack from the primary amino groups of heptakis(6-deoxy-6-amino)-βcyclodexttrin (NH-CD) to form NH-CD-P(ES).…”

Section: Bioapplications Of Sr-ppmecsmentioning

confidence: 99%

“…It is difficult to control the reversible modification of the membranes to regenerate the enzyme activity in situ. On the other hand, using the fluorescence intensity changes to monitor the enzyme renewal process in PPMECs is an attractive protocol. ,, …”

Section: Bioapplications Of Sr-ppmecsmentioning

confidence: 99%

Design of Switchable Enzyme Carriers Based on Stimuli-Responsive Porous Polymer Membranes for Bioapplications

Qiao

2021

ACS Appl. Bio Mater.

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Design of efficient enzyme carriers, where enzymes are conjugated to supports, has become an attractive research avenue. Immobilized enzymes are advantageous for practical applications because of their convenience in handling, ease of separation, and good reusability. However, the main challenge is that these traditional enzyme carriers are unable to regulate the enzymolysis efficiency or to protect the enzymes from proteolytic degradation, which restricts their effectiveness of enzymes in bioapplications. Enlightened by the stimuli-responsive channels in the natural cell membranes, conjugation of the enzymes within flat-sheet stimuli-responsive porous polymer membranes (SR-PPMs) as artificial cell membranes is an efficient strategy for circumventing this challenge. Controlled by the external stimuli, the multifunctional polymer chains, which are incorporated within the membranes and attached to the enzyme, change their structures to defend the enzyme from the external environmental disturbances and degradation by proteinases. Specifically, smart SR-PPM enzyme carriers (SR-PPMECs) not only permit convective substrate transfer through the accessible porous network, dramatically improving enzymolysis efficiency due to the adjustable pore sizes and the confinement effect, but they also act as molecular switches for regulating its permeability and selectivity. In this review, the concept of SR-PPMECs is presented. It covers the latest developments in design strategies of flat-sheet SR-PPFMs, fabrication protocols of SR-PPFMECs, strategies for the regulation of enzymolysis efficiency, and their cutting-edge bioapplications.

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“…At 378C (above the LCST of the grafted polymer), Con A was exposed on the surface to capture the nattokinase-loaded RBCs and displayed the fibrinolytic property. However, at 48C, PNiPAAm-co-AAc underwent conformational changes and therefore the conjugated Con A has reverted inside the matrix and released the enzyme-loaded RBCs [39]. Here, RBCs were used as a carrier for enzyme encapsulation, which proved to be potent biocompatible carriers for delivery of biomolecules [40,41].…”

Section: Therapeutic Biocatalystsmentioning

confidence: 99%

An update on smart biocatalysts for industrial and biomedical applications

Shakya

Nandakumar

2018

J. R. Soc. Interface.

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Recently, smart biocatalysts, where enzymes are conjugated to stimuli-responsive (smart) polymers, have gained significant attention. Based on the presence or absence of external stimuli, the polymer attached to the enzyme changes its conformation to protect the enzyme from the external environment and regulate the enzyme activity, thus acting as a molecular switch. Owing to this behaviour, smart biocatalysts can be separated easily from a reaction mixture and re-used several times. Several such smart polymer-based biocatalysts have been developed for industrial and biomedical applications. In addition, they have been used in biosensors, biometrics and nano-electronic devices. This review article covers recent advances in developing different kinds of stimuli-responsive enzyme bioconjugates, including conjugation strategies, and their applications.

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Immobilization of nattokinase-loaded red blood cells on the surface of superhydrophobic polypropylene targeting fibrinolytic performance

Abstract: A superhydrophobic polypropylene (PP) platform with fibrinolytic ability was fabricated by capturing and releasing nattokinase (NK)-encapsulating red blood cells (RBCs).

Cited by 13 publications

References 37 publications

Thermally Stable Polypropylene Superhydrophobic Surface Due to the Formation of a Surface Crystalline Layer of Microsized Particles

Thermally Stable Polypropylene Superhydrophobic Surface Due to the Formation of a Surface Crystalline Layer of Microsized Particles

Design of Switchable Enzyme Carriers Based on Stimuli-Responsive Porous Polymer Membranes for Bioapplications

An update on smart biocatalysts for industrial and biomedical applications

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