Immobilization of metallothionein as a sensitive biosensor chip for the detection of metal ions by surface plasmon resonance

Wu, Chien-Hou; Lin, Lih-Yuan

doi:10.1016/j.bios.2004.03.026

Cited by 79 publications

(37 citation statements)

References 28 publications

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“…The buffer components play a key role in the analysis of binding affinity between protein and metal ions [10,19]. We have mimicked the serum environment to investigate the interaction between metals and albumin.…”

Section: Discussionmentioning

confidence: 99%

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Utilization of albumin-based sensor chips for the detection of metal content and characterization of metal–protein interaction by surface plasmon resonance

Wu¹,

Lin²

2005

Sensors and Actuators B: Chemical

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Section: Discussionmentioning

confidence: 99%

“…The level and affinity of the binding can thus be monitored by SPR and estimated. Recently, we applied this technique to analyze metal concentrations in solutions by immobilizing mammalian metallothionein on sensor chips [10]. Using this sensor chip, we can detect several species of metals with different binding affinity.…”

Section: Introductionmentioning

confidence: 99%

Utilization of albumin-based sensor chips for the detection of metal content and characterization of metal–protein interaction by surface plasmon resonance

Wu¹,

Lin²

2005

Sensors and Actuators B: Chemical

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“…A biosensor based on mammalian metallothionein (MT) for the detection of metal ions was developed and characterized (Wu and Lin 2004). MT was immobilized onto a carboxymethylated dextran matrix as a biosensor for the detection of metal ions by surface plasmon resonance (SPR).…”

Section: Detection Of Heavy Metals Adulterants and Other Toxic Compomentioning

confidence: 99%

Optical biosensors for food quality and safety assurance—a review

et al. 2011

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Food quality and safety is a scientific discipline describing handling, preparation and storage of food in ways that prevent food borne illness. Food serves as a growth medium for microorganisms that can be pathogenic or cause food spoilage. Therefore, it is imperative to have stringent laws and standards for the preparation, packaging and transportation of food. The conventional methods for detection of food contamination based on culturing, colony counting, chromatography and immunoassay are tedious and time consuming while biosensors have overcome some of these disadvantages. There is growing interest in biosensors due to high specificity, convenience and quick response. Optical biosensors show greater potential for the detection of pathogens, pesticide and drug residues, hygiene monitoring, heavy metals and other toxic substances in the food to check whether it is safe for consumption or not. This review focuses on optical biosensors, the recent developments in the associated instrumentation with emphasis on fiber optic and surface plasmon resonance (SPR) based biosensors for detecting a range of analytes in food samples, the major advantages and challenges associated with optical biosensors. It also briefly covers the different methods employed for the immobilization of bio-molecules used in developing biosensors.

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“…It has been shown previously that MT-based biosensors can be utilized for detection of various heavy metal ions and their compounds [1,26,[33][34][35]. Metallothionein studied by differential pulse voltammetry gives several electrochemical signals: MT(Cd), MT(Zn), ZnT, and FreeT.…”

Section: Mt-based Biosensor For Silver(i) Ion Detectionmentioning

confidence: 99%

Protein‐based electrochemical biosensor for detection of silver(I) ions

Křížková

Húska

Beklová

et al. 2009

Enviro Toxic and Chemistry

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Silver(I) ions are extremely toxic to aquatic animals. Hence, monitoring of these ions in the environment is needed. The aim of the present study was to suggest a simple biosensor for silver(I) ions detection. The suggested biosensor is based on the modification of a hanging mercury drop electrode (HMDE) by the heavy metal binding protein metallothionein (MT) for silver(I) ions detection. Metallothionein accumulated for 120 s onto the HMDE surface. After rinsing the electrode, the biosensor (MT modified HMDE) was prepared prior to detection of silver(I) ions. The biosensor was immersed in a solution containing silver(I) ions. These ions were bound to the MT structure. Furthermore, the electrode was rinsed and transferred to a pure supporting electrolyte solution, in which no interference was present. Under these experimental conditions, other signals relating to heavy metals naturally occurring in MT were not detected. This phenomenon confirms the strong affinity of silver(I) ions for MT. The suggested biosensor responded well to higher silver(I) ion concentrations. The relative standard deviation for measurements of concentrations higher than 50 microM was approximately 2% (n = 8). In the case of concentrations lower than 10 microM, the relative standard deviation increased to 10% (n = 8). The detection limit (3 signal/noise) for silver(I) ions was estimated as 500 nM.

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Immobilization of metallothionein as a sensitive biosensor chip for the detection of metal ions by surface plasmon resonance

Cited by 79 publications

References 28 publications

Utilization of albumin-based sensor chips for the detection of metal content and characterization of metal–protein interaction by surface plasmon resonance

Utilization of albumin-based sensor chips for the detection of metal content and characterization of metal–protein interaction by surface plasmon resonance

Optical biosensors for food quality and safety assurance—a review

Protein‐based electrochemical biosensor for detection of silver(I) ions

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