Abstract:Enzyme-linked immunosorbent assays (ELISAs) can be designed to detect either antigens or antibodies. Nearly all ELISA formats require the separation of reactants from the products of the immunoassay. The product of the assay is an immune complex consisting of target ligand, the analyte, and the reporting probe used to detect the complex that is formed. Because it 1s usually necessary to remove excess analyte as well as excess probe before measurements, several separation steps may be desired, if not required. … Show more
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