Immobilization of lipase on epoxy-activated Purolite® A109 and its post-immobilization stabilization

Lipase immobilization on epoxy-activated poly(vinyl acetate-acrylamide) microspheres

“…The immobilized lipase has exhibited a high specific activity of 2.9 U/mg lipase at 76 mol/g microsphere, which suggested that epoxy-covalent immobilization of lipase was a proper enzyme immobilization way because the mild immobilization reaction condition did not destroy enzyme activity [28]. Similar observations were also reported previously by Bezbradica and his colleagues [14] who showed that epoxy-activated Purolite could be well used as carrier to immobilize lipase, and a high protein loading capacity and high lipase activity were obtained. Besides, Tural's group [13,29,30] also demonstrated that decarboxylase from Pseudomonas putida and benzaldehyde lyase immobilized on epoxy-activated magnetic support exhibited a high enzyme activity recovery.…”

“…Covalent immobilization provided a good strategy for enzyme immobilization by preventing the shedding and leakage of enzyme compared with physical absorption [10][11][12]. Particularly, epoxy-activated carriers could be utilized to covalently immobilize enzyme under mild reaction conditions [13,14]. Therefore, exploiting good carriers and immobilization strategies has become an interesting work for enzyme immobilization.…”

HaloTag™: Evaluation of a covalent one-step immobilization for biocatalysis

“…. One option to achieve the direct immobilization of enzymes directly from crude cell extracts is the selective absorption through non-covalent or covalent binding on hydrophobic supports that were previously reported for lipases (Bastida et al, 1998;Mihailović et al, 2014) However, a selective method combining purification and covalent site-directed immobilization for a broad range of enzymes in one step is highly demanded. Therefore, fusions of catalysts with respective protein tags are useful (Barbosa et al, 2015).…”

Physical-Chemical Properties of the Support Immobead 150 Before and After the Immobilization Process of Lipase

“…1,2 One of the most important aspects for the development of different supports for enzyme immobilization is their possible reuse in repeated batch cycles or in continuous process. 3 Supports must present sufficient amount of functional groups on their surfaces, allowing interaction with enzyme molecules. Furthermore, supports should have mechanical and morphological properties that allow their use under industrial conditions, where shear force and temperature stresses are often present.…”

“…7,8 A variety of matrices have been used as support materials for enzyme immobilization. 3,[9][10][11][12] Any solid material that contains cavities, channels, or interstices may be regarded as porous, which is a property of major importance for the practical applications of support materials. 13 According to IUPAC recommendations, 13 pores with free diameters smaller than 2 nm are classified as micropores, those in the range of 2 to 50 nm are mesopores, and those larger than 50 nm, macropores.…”