Immobilization of lignin peroxidase on nanoporous gold: Enzymatic properties and in situ release of H2O2 by co-immobilized glucose oxidase

Qiu, Hua‐Jun; Li, Ying; Ji, Guanglei; Zhou, Guiping; Huang, Xirong; Qu, Yinbo; Gao, Pin

doi:10.1016/j.biortech.2009.03.016

Cited by 68 publications

(39 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…oxidase [134,140,141], alcohol dehydrogenase [141], xylanase [142], acetylcholine esterase [66], cytochrome c [135], ligin peroxidase [143], lipase [139], catalase [139], horseradish peroxidase [139], bovine serum albumin [90], and immunoglobulin G [90]. Strategies for immobilization have included physical, electrostatic, and covalent attachment.…”

Section: Dna Sensorsmentioning

confidence: 99%

“…The ability to use an electrode that has an electrochemically useful surface area that is 10-100 times larger than a traditional electrode thus offers much promise. Examples of small molecules that have been studied include dopamine [144], ascorbic acid [144], hydrogen peroxide [143,145], nitrite [146], cysteine [147], nitrophenol [148,149], hydrazine [150], and glucose [151].…”

Section: Small Molecule Sensingmentioning

confidence: 99%

See 1 more Smart Citation

Nanoporous Gold Electrodes and Their Applications in Analytical Chemistry

Collinson

2013

ISRN Analytical Chemistry

View full text Add to dashboard Cite

Nanoporous gold prepared by dealloying Au:Ag alloys has recently become an attractive material in the field of analytical chemistry. This conductive material has an open, 3D porous framework consisting of nanosized pores and ligaments with surface areas that are 10s to 100s of times larger than planar gold of an equivalent geometric area. The high surface area coupled with an open pore network makes nanoporous gold an ideal support for the development of chemical sensors. Important attributes include conductivity, high surface area, ease of preparation and modification, tunable pore size, and a bicontinuous open pore network. In this paper, the fabrication, characterization, and applications of nanoporous gold in chemical sensing are reviewed specifically as they relate to the development of immunosensors, enzyme-based biosensors, DNA sensors, Raman sensors, and small molecule sensors.

show abstract

Section: Dna Sensorsmentioning

confidence: 99%

Section: Small Molecule Sensingmentioning

confidence: 99%

Nanoporous Gold Electrodes and Their Applications in Analytical Chemistry

Collinson

2013

ISRN Analytical Chemistry

View full text Add to dashboard Cite

show abstract

“…Meanwhile, lignin peroxidase (LiP) can oxidize a wide range of environmentally persistent aromatic compounds and, therefore, many attempts have been made to degrade aromatic pollutants with this enzyme (84). Qia and co-workers demonstrated that Lip immobilized nanoporous gold revealed a potential of very effective decolourization of aromatic dye molecules such as fushcine, rhodamine B and pyrogallol red (13). http://bmbreports.org BMB reports…”

Section: Bioenergy and Environmental Technologymentioning

confidence: 99%

Inorganic nanomaterial-based biocatalysts

Lee

Lee²,

Chang³

et al. 2011

BMB Reports

View full text Add to dashboard Cite

Over the years, nanostructures have been developed to enable to support enzyme usability to obtain highly selective and efficient biocatalysts for catalyzing processes under various conditions. This review summarizes recent developments in the nanostructures for enzyme supporters, typically those formed with various inorganic materials. To improve enzyme attachment, the surface of nanomaterials is properly modified to express specific functional groups. Various materials and nanostructures can be applied to improve both enzyme activity and stability. The merits of the incorporation of enzymes in inorganic nanomaterials and unprecedented opportunities for enhanced enzyme properties are discussed.

show abstract

“…10% of the initial activity was lost after 2 h incubation. The greatly improved thermal stability should be due to the multipoint attachment between HRP and the NPC surface (Kumar et al, 2010;Qiu et al, 2009a;Vaidya et al, 2008;Wang et al, 2001), which made the 3-dimentional structure of HRP more stable. The greatly improved thermal stability of the immobilized enzyme is very important for the practical industrial applications, in which usually high temperatures are needed to enhance the reaction rate, conversion and the solubility of some reactants.…”

Section: Properties Of the Immobilized Hrpmentioning

confidence: 99%