Immobilization of laccase on magnetically separable biochar for highly efficient removal of bisphenol A in water

Zhang, Yu; Piao, Mingyue; He, Longhui; Yao, Lan; Piao, Tiezhu; Liu, Zairan; Piao, Yunxian

doi:10.1039/c9ra08800h

Cited by 64 publications

(18 citation statements)

References 49 publications

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“…Hence, the obtained results in Figure 6 B suggest significantly improved thermal stability of the laccase after the immobilization protocol. There are many applications of the enzyme laccase that can be enhanced by improved thermal stability [ 35 ]. Further, the storage stability of the immobilized and free laccase was tested ( Figure 6 C).…”

Section: Resultsmentioning

confidence: 99%

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Thiolation of Chitosan Loaded over Super-Magnetic Halloysite Nanotubes for Enhanced Laccase Immobilization

et al. 2020

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This study focuses on the development of a nanosupport based on halloysite nanotubes (HNTs), Fe3O4 nanoparticles (NPs), and thiolated chitosan (CTs) for laccase immobilization. First, HNTs were modified with Fe3O4 NPs (HNTs-Fe3O4) by the coprecipitation method. Then, the HNTs-Fe3O4 surface was tuned with the CTs (HNTs-Fe3O4-CTs) by a simple refluxing method. Finally, the HNTs- Fe3O4-CTs surface was thiolated (-SH) (denoted as; HNTs- Fe3O4-CTs-SH) by using the reactive NHS-ester reaction. The thiol-modified HNTs (HNTs- Fe3O4-CTs-SH) were characterized by FE-SEM, HR-TEM, XPS, XRD, FT-IR, and VSM analyses. The HNTs-Fe3O4-CTs-SH was applied for the laccase immobilization. It gave excellent immobilization of laccase with 100% activity recovery and 144 mg/g laccase loading capacity. The immobilized laccase on HNTs-Fe3O4-CTs-SH (HNTs-Fe3O4-CTs-S-S-Laccase) exhibited enhanced biocatalytic performance with improved thermal, storage, and pH stabilities. HNTs-Fe3O4-CTs-S-S-Laccase gave outstanding repeated cycle capability, at the end of the 15th cycle, it kept 61% of the laccase activity. Furthermore, HNTs-Fe3O4-CTs-S-S-Laccase was applied for redox-mediated removal of textile dye DR80 and pharmaceutical compound ampicillin. The obtained result marked the potential of the HNTs-Fe3O4-CTs-S-S-Laccase for the removal of hazardous pollutants. This nanosupport is based on clay mineral HNTs, made from low-cost biopolymer CTs, super-magnetic in nature, and can be applied in laccase-based decontamination of environmental pollutants. This study also gave excellent material HNTs-Fe3O4-CTs-SH for other enzyme immobilization processes.

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Section: Resultsmentioning

confidence: 99%

“…Further, the storage stability of the immobilized and free laccase was tested ( Figure 6 C). The long-term storage of the enzyme in solution is a challenging task [ 35 ]. However, after immobilization, the enzyme native structure can be protected for a longer time [ 10 ].…”

Section: Resultsmentioning

confidence: 99%

Thiolation of Chitosan Loaded over Super-Magnetic Halloysite Nanotubes for Enhanced Laccase Immobilization

et al. 2020

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“…Hence, the detailed activity recovery and laccase loading capacity data provided the information about immobilization behavior of α-Cellulose-Fe3O4-CTNs-SH. The significant laccase loading obtained by α-Cellulose-Fe3O4-CTNs-SH was quite high among the recently reported materials [33,46,[54][55][56][57][58][59][60][61]. Thus, the developed α-cellulose based material has significant potential to look like an enzyme immobilization material.…”

Section: Activity Recovery and Laccase Loading Capacity Evaluationsmentioning

confidence: 92%

α-Cellulose Fibers of Paper-Waste Origin Surface-Modified with Fe3O4 and Thiolated-Chitosan for Efficacious Immobilization of Laccase

et al. 2021

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The utilization of waste-paper-biomass for extraction of important α-cellulose biopolymer, and modification of extracted α-cellulose for application in enzyme immobilization can be extremely vital for green circular bio-economy. Thus, in this study, α-cellulose fibers were super-magnetized (Fe3O4), grafted with chitosan (CTNs), and thiol (-SH) modified for laccase immobilization. The developed material was characterized by high-resolution transmission electron microscopy (HR-TEM), HR-TEM energy dispersive X-ray spectroscopy (HR-TEM-EDS), X-ray diffraction (XRD), vibrating sample magnetometer (VSM), X-ray photoelectron spectroscopy (XPS), and Fourier transform infrared spectroscopy (FT-IR) analyses. Laccase immobilized on α-Cellulose-Fe3O4-CTNs (α-Cellulose-Fe3O4-CTNs-Laccase) gave significant activity recovery (99.16%) and laccase loading potential (169.36 mg/g). The α-Cellulose-Fe3O4-CTNs-Laccase displayed excellent stabilities for temperature, pH, and storage time. The α-Cellulose-Fe3O4-CTNs-Laccase applied in repeated cycles shown remarkable consistency of activity retention for 10 cycles. After the 10th cycle, α-Cellulose-Fe3O4-CTNs possessed 80.65% relative activity. Furthermore, α-Cellulose-Fe3O4-CTNs-Laccase shown excellent degradation of pharmaceutical contaminant sulfamethoxazole (SMX). The SMX degradation by α-Cellulose-Fe3O4-CTNs-Laccase was found optimum at incubation time (20 h), pH (3), temperatures (30 °C), and shaking conditions (200 rpm). Finally, α-Cellulose-Fe3O4-CTNs-Laccase gave repeated degradation of SMX. Thus, this study presents a novel, waste-derived, highly capable, and super-magnetic nanocomposite for enzyme immobilization applications.

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“…In addition, after combining the biochar-loaded laccase with iron, the composite obtained even more interesting results. For instance, Zhang et al, immobilized laccase on a cost-effective and nanosized magnetic biochar (L-MBC) by adsorption, precipitation, and crosslinking, and used it for high performance BPA removal [ 45 ]. All of these studies state that the biochar-loaded enzyme has good potential for the application in fabricating electrochemical biosensors.…”

Section: The Role Of Biochar In the Fabrication Of Electrochemical Se...mentioning

confidence: 99%

Recent Advances of Biochar-Based Electrochemical Sensors and Biosensors

Wang

et al. 2022

Biosensors

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In the context of accelerating the global realization of carbon peaking and carbon neutralization, biochar produced from biomass feedstock via a pyrolysis process has been more and more focused on by people from various fields. Biochar is a carbon-rich material with good properties that could be used as a carrier, a catalyst, and an absorbent. Such properties have made biochar a good candidate as a base material in the fabrication of electrochemical sensors or biosensors, like carbon nanotube and graphene. However, the study of the applications of biochar in electrochemical sensing technology is just beginning; there are still many challenges to be conquered. In order to better carry out this research, we reviewed almost all of the recent papers published in the past 5 years on biochar-based electrochemical sensors and biosensors. This review is different from the previously published review papers, in which the types of biomass feedstock, the preparation methods, and the characteristics of biochar were mainly discussed. First, the role of biochar in the fabrication of electrochemical sensors and biosensors is summarized. Then, the analytes determined by means of biochar-based electrochemical sensors and biosensors are discussed. Finally, the perspectives and challenges in applying biochar in electrochemical sensors and biosensors are provided.

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Immobilization of laccase on magnetically separable biochar for highly efficient removal of bisphenol A in water

Abstract: Laccase was stably immobilized on a cost effective and nanosized magnetic biochar (L-MBC) by adsorption, precipitation and crosslinking, and it was used for high performance BPA removal.

Cited by 64 publications

References 49 publications

Thiolation of Chitosan Loaded over Super-Magnetic Halloysite Nanotubes for Enhanced Laccase Immobilization

Thiolation of Chitosan Loaded over Super-Magnetic Halloysite Nanotubes for Enhanced Laccase Immobilization

α-Cellulose Fibers of Paper-Waste Origin Surface-Modified with Fe3O4 and Thiolated-Chitosan for Efficacious Immobilization of Laccase

Recent Advances of Biochar-Based Electrochemical Sensors and Biosensors

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