Immobilization of invertase within calcium alginate gel capsules

Tanrıseven, Aziz; Doğan, Şenay

doi:10.1016/s0032-9592(01)00146-7

Cited by 107 publications

(65 citation statements)

References 8 publications

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“…However, it was found that the immobilization of biocatalysts, including phytase, is an essential factor for their stabilization. For example, the immobilization of enzymes by covalent binding enables their reuse several times, which results in the reduction of their total cost as well as that of their products [7,8]. It also has the advantage of keeping the enzymes well bound to the carrier and hence avoids their diff usion into the fermentation medium.…”

Section: Introductionmentioning

confidence: 99%

Comparative studies of free and immobilized phytase, produced by Penicillium purpurogenu GE1, using grafted alginate/carrageenan beads

Awad¹,

Esawy²,

El-Gammal³

et al. 2015

Egypt Pharmaceut J

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Section: Introductionmentioning

confidence: 99%

Comparative studies of free and immobilized phytase, produced by Penicillium purpurogenu GE1, using grafted alginate/carrageenan beads

Awad¹,

Esawy²,

El-Gammal³

et al. 2015

Egypt Pharmaceut J

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“…Inorganic supports provide better stability features and hence there is immense scope for research in this area. Porous glass (Ooshima, Sakimoto, & Harano, 1980), alginate gels (Tanriseven & Dogan, 2001) and rice husk (DÕSouza & Godbole, 2002) are some of the supports on which invertase immobilization has been carried out.…”

Section: Introductionmentioning

confidence: 99%

Enhanced pH and thermal stabilities of invertase immobilized on montmorillonite K-10

Gopinath

Sugunan

2006

Food Chemistry

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Invertase was adsorbed onto micro-porous acid-activated montmorillonite clay (K-10) by two procedures, namely adsorption and covalent binding. The immobilized enzymes were characterized by XRD, surface area measurements and 27 Al NMR. XRD measurements revealed an expansion of clay layers due to immobilization which suggests that intercalation had taken place. Surface area measurements also support this observation. 27 Al NMR showed that interaction of enzyme with tetrahedral and octahedral Al changes with the immobilization procedure. Sucrose hydrolysis was performed in a batch reactor. The immobilized enzymes showed enhanced pH and thermal stabilities. Optimum pH and temperature were found to increase upon immobilization. The effectiveness factor (g) and Michaelis constant (K m ) suggest that diffusional resistances play a major role in the reaction. The immobilized invertase could be stored in buffer of pH 5 and 6 at 5°C without any significant loss in activity for 20 days.

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“…Soluble invertase is used in the sweet industry in the production of artificial honey. Immobilisation of invertase on corn grits [3], polymer matrices [4][5][6][7], calcium alginate [8], composite gel fibres [9] etc., has already been reported. Studies on reusability of the immobilised enzymes are also reported.…”

Section: Introductionmentioning

confidence: 99%

Invertase immobilised on montmorillonite: reusability enhancement and reduction in leaching

Gopinath

Sugunan

2005

Catalysis Communications

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Invertase was immobilised on microporous montmorillonite K-10 via adsorption and covalent binding. The immobilised enzymes were tested for sucrose hydrolysis activity in a batch reactor. K m for immobilised systems was greater than free enzyme. The immobilised forms could be reused for 15 continuous cycles without any loss in activity. After 25 cycles, 85% initial activity was retained. A study on leaching of enzymes showed that 100% enzyme was retained even after 15 cycles of reuse. Leaching increased with reaction temperature. Covalent binding resisted leaching even at temperatures of 70°C.

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Immobilization of invertase within calcium alginate gel capsules

Cited by 107 publications

References 8 publications

Comparative studies of free and immobilized phytase, produced by Penicillium purpurogenu GE1, using grafted alginate/carrageenan beads

Comparative studies of free and immobilized phytase, produced by Penicillium purpurogenu GE1, using grafted alginate/carrageenan beads

Enhanced pH and thermal stabilities of invertase immobilized on montmorillonite K-10

Invertase immobilised on montmorillonite: reusability enhancement and reduction in leaching

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