Immobilization ofPaecilomyces variotiitannase and properties of the immobilized enzyme

Abstract

Tannase produced by Paecilomyces variotii was encapsulated in sodium alginate beads and used for the effective hydrolysis of tannic acid; the efficiency of hydrolysis was comparable to that of the free enzyme. The alginate beads retained 100% of their efficiency in the first three rounds of successive use and 60% in rounds 4 and 5. The response surface methodology showed that the best conditions to hydrolysis of tannic acid by immobilized tannase were: sodium alginate 5.2%, CaCl₂ 0.55 M and 9 h to curing time. The optimized process resulted in 2.4 times more hydrolysed tannic acid than that obtained before optimization. The optimum pH for the actions of both the encapsulated and the free enzymes was 5.5. The optimum temperature of the reaction was determined to be 40 °C for the free enzyme and 60 °C for the immobilized form. The immobilization process improved the stability at low pH.

Biotechnological Advances and Challenges of Tannase: An Overview

“…In this regard, tannase immobilization offers several advantages over the utilization of free enzyme, such as improvement of enzyme stability, reutilization of biocatalyst, ease of product recovery, and continuous operation in packed bed bioreactors (Schons et al 2011).…”

Characterization of a glucose- and solvent-tolerant extracellular tannase from Aspergillus phoenicis

“…Some tannases, due to the presence of 1-propanol, are able to act as transferase in an organic reaction medium to produce propyl-gallate, an important food additive and an antioxidant for lipid-rich products [1]. Several microorganisms have been identified as sources of tannases, including the filamentous fungi Aspergillus awamori [4], Paecilomyces variotii [5], Aspergillus niger [6] and Emericella nidulans [7], among others. The production of tannase is achieved through submerged fermentation [4,7] or solid-state fermentation [8,9] using different substrates/carbon sources.…”

Biotransformation of industrial tannins by filamentous fungi

“…Particular attention is given to those enzymes with better catalytic features. Tannase from P. variotii showed 95% of activity recovery in the range of 3.5-7.5 pH and 20-50°C (Schons et al 2011). The Basidiomycota L. elegans secreted a tannase effective from 40 to 60°C, whose activity was only halved at 80°C (Ordonez et al 2011).…”

“…Due to their biochemical properties, the demand for industrial enzymes is increasing, asking for the (Ong and Annuar et al 2018) and enhances the enzymatic stability, enlarging the pH and temperature range of action (Yu et al 2004;Schons et al 2011;Kumar et al 2015). Immobilization can also protect the enzymes from inhibition effects played by metal ions.…”