Immobilization of Horseradish Peroxidase on Multi-Armed Magnetic Graphene Oxide Composite

Rong, Junhui; Zhou, Zijuan; Wang, Yun; Han, Juan; Li, Chunmei; Zhang, Wenli; Ni, Liang

doi:10.17113/ftb.57.02.19.5832

Cited by 19 publications

(22 citation statements)

References 31 publications

(30 reference statements)

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“…Other supports showed a similar trend, ref. [54] indicating a reduction of the catalytic efficiency due to mass transfer limitation processes [55] and restriction of the mobility of the enzyme [56].…”

Section: Activity Parameters and Kinetic Data Of Biocatalysts I-xmentioning

confidence: 99%

Oxidative Bio-Desulfurization by Nanostructured Peroxidase Mediator System

et al. 2020

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Bio-desulfurization is an efficient technology for removing recalcitrant sulfur derivatives from liquid fuel oil in environmentally friendly experimental conditions. In this context, the development of heterogeneous bio-nanocatalysts is of great relevance to improve the performance of the process. Here we report that lignin nanoparticles functionalized with concanavalin A are a renewable and efficient platform for the layer-by-layer immobilization of horseradish peroxidase. The novel bio-nanocatalysts were applied for the oxidation of dibenzothiophene as a well-recognized model of the recalcitrant sulfur derivative. The reactions were performed with hydrogen peroxide as a green primary oxidant in the biphasic system PBS/n-hexane at 45 °C and room pressure, the highest conversion of the substrate occurring in the presence of cationic polyelectrolyte layer and hydroxy-benzotriazole as a low molecular weight redox mediator. The catalytic activity was retained for more transformations highlighting the beneficial effect of the support in the reusability of the heterogeneous system.

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Section: Activity Parameters and Kinetic Data Of Biocatalysts I-xmentioning

confidence: 99%

Oxidative Bio-Desulfurization by Nanostructured Peroxidase Mediator System

et al. 2020

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“…Until now, various substrates have been used to immobilize enzymes ( 13 ). These substrates should have the following basic properties: availability, tendency to bind to proteins, presence of free working groups and reactions with the target enzyme, hydrophilic strength, mechanical stability, tissue stiffness, flexibility in different geometrical structures, permeability, and appropriate surface for transfer, degradability, cost-effectiveness and safety of application ( 14 , 15 ).…”

Section: Introductionmentioning

confidence: 99%

Magnetic Biocatalysts of Pectinase: Synthesis by Macromolecular Cross-Linker for Application in Apple Juice Clarification

Nouri

Khodaiyan

2020

Food Technol. Biotechnol. (Online)

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Research background. Pectinase enzyme has become a valuable compound in beverage industry. One of the most significant concepts to overcome the drawbacks of using industrial enzymes is the immobilization of enzymes. In the present study, magnetic chitosan microparticles were utilized as a substrate for pectinase enzyme immobilization. New methods of enzyme immobilization involve the use of non-chemical cross-linkers between the enzyme and the substrate. The aim of this study was to immobilize the pectinase enzyme using polyaldehyde kefiran as a macromolecular crosslinker on magnetic particles. Experimental approach. Pectinase enzyme was immobilized in four steps: relative oxidation of kefiran and its application as a crosslinker; production of magnetic iron (II, III) oxide (Fe3O4) microparticles; coating of magnetic Fe3O4 microparticles with chitosan; and immobilization of enzyme on the substrate, prepared by the use of oxidized kefiran crosslinker. Parameters, such as crosslinking concentration, time, and ratio of chitosan magnetic microparticles to enzyme, were optimized. Fourier-transform infrared spectroscopy (FTIR), dynamic light scattering (DLS), transmission electron microscopy (TEM), and vibrating sample magnetometer (VSM) were used to identify the groups and investigate the structures. The biochemical properties (stability of enzyme activity at different pH, temperature and time), enzyme reusability, kinetics parameters (Km and Vmax) and apple juice turbidity, using free and immobilized pectinase enzymes, were also measured. Results and conclusions. Cross-linker concentration, crosslinking time and ratio of CMMP to enzyme were important factors in activity recovery of pectinase enzyme. FTIR analysis correctly identified functional groups in the structures. The results showed that after enzyme stabilization, the particle size and molecular mass increased and decreased the magnetic saturation strength, respectively. According to the thermal kinetic study, the immobilized pectinase was higher than its free form. In the present study, findings indicate the excellent stability and durability of the immobilized pectinase. Findings indicate the excellent stability and durability of the immobilized pectinase in present study. Finally, a magnetic pectinase micro-biocatalyst was used to clarify apple juice, which reduced turbidity of processing. Novelty and scientific contribution. This study investigates the usage of kefiran oxidized as a new cross linker for the immobilization of pectinase enzyme. Magnetic pectinase micro-biocatalyst has a good potential for industrial applications in the food industry, with high thermal stability.

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“…Hyperbranched polymers, such as multi-arm polyethylene glycol amine (PEG), containing a large number of amino groups, can be employed to fabricate the surface of magnetic nanomaterials and increase their capacity for enzyme loading. It can also increase enzyme catalytic activity and make the catalytic reactions of enzymes low-cost [ 31 , 32 , 33 ]. This polymer is highly water-soluble and biocompatible, enabling it to enhance the carrier’s water-soluble and biocompatible properties and protect the carrier structure, resulting in increased immobilized enzyme activity and stability [ 34 ].…”

Section: Introductionmentioning

confidence: 99%

“…This compound contains many amino groups, and can, thus, create an immobilized enzyme when glutaraldehyde (a cross-linking agent) is bound with protein, increasing the enzyme’s stability and loading capacity. Additionally, 4-arm-PEG-NH 2 can create a stable amide bond with the carboxyl group of materials, enhancing their solubility and stability [ 33 ]. To our knowledge, modification of magnetic MWCNTs by 4-arm-PEG-NH 2 polymer, and their application for co-immobilization lipases have not been reported previously.…”

Section: Introductionmentioning

confidence: 99%

Co-Immobilization of Rhizopus oryzae and Candida rugosa Lipases onto mMWCNTs@4-arm-PEG-NH2—A Novel Magnetic Nanotube–Polyethylene Glycol Amine Composite—And Its Applications for Biodiesel Production

Abdulmalek

Wang

et al. 2021

IJMS

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This article describes the successful synthesis of a novel nanocomposite of superparamagnetic multi-walled nanotubes with a four-arm polyethylene glycol amine polymer (mMWCNTs@4-arm-PEG-NH2). This composite was then employed as a support for the covalent co-immobilization of Rhizopus oryzae and Candida rugosa lipases under appropriate conditions. The co-immobilized lipases (CIL-mMWCNTs@4-arm-PEG-NH2) exhibited maximum specific activity of 99.626U/mg protein, which was 34.5-fold superior to that of free ROL, and its thermal stability was greatly improved. Most significantly, CIL-mMWCNTs@4-arm-PEG-NH2 was used to prepare biodiesel from waste cooking oil under ultrasound conditions, and within 120 min, the biodiesel conversion rate reached 97.64%. This was due to the synergy effect between ROL and CRL and the ultrasound-assisted enzymatic process, resulting in an increased biodiesel yield in a short reaction time. Moreover, after ten reuse cycles, the co-immobilized lipases still retained a biodiesel yield of over 78.55%, exhibiting excellent operational stability that is attractive for practical applications. Consequently, the combined use of a novel designed carrier, the co-immobilized lipases with synergy effect, and the ultrasound-assisted enzymatic reaction exhibited potential prospects for future applications in biodiesel production and various industrial applications.

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Immobilization of Horseradish Peroxidase on Multi-Armed Magnetic Graphene Oxide Composite

Cited by 19 publications

References 31 publications

Oxidative Bio-Desulfurization by Nanostructured Peroxidase Mediator System

Oxidative Bio-Desulfurization by Nanostructured Peroxidase Mediator System

Magnetic Biocatalysts of Pectinase: Synthesis by Macromolecular Cross-Linker for Application in Apple Juice Clarification

Co-Immobilization of Rhizopus oryzae and Candida rugosa Lipases onto mMWCNTs@4-arm-PEG-NH2—A Novel Magnetic Nanotube–Polyethylene Glycol Amine Composite—And Its Applications for Biodiesel Production

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