Immobilization of glucose oxidase within calcium alginate gel capsules

Blandino, Ana; Macı́as, M.; Cantero, Domingo

doi:10.1016/s0032-9592(00)00240-5

Cited by 177 publications

(122 citation statements)

References 18 publications

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“…The results were in agreement with previous studies [12,[15][16][17]. It has been reported that the membrane thickness of the capsules was increased as the calcium chloride concentration increased [12,16,17].…”

Section: Influence Of Liquid Core Solution Formulationsupporting

confidence: 93%

“…The membrane thickness of the capsules also exhibited a decrease as alginate concentration increased (see Figure 3B) for a given gelation time. The results were in good agreement with previous studies [12,16,17]. At high alginate concentrations, number of alginate molecules per unit volume and the number of binding sites for Ca 2+ cation also increased.…”

Section: Influence Of Alginate Solution Formulationsupporting

confidence: 92%

“…For a given dripping tip diameter, the capsule diameter and membrane thickness increases when the gelation time increases. The results were in good agreement with previous studies [6,11,12,17]. After the core solution droplet detached from the dripping tip, it is speculated that instantaneous gelation occurred upon the droplet colliding with the alginate solution [11,12].…”

Section: Influence Of Gelation Timesupporting

confidence: 92%

“…At high alginate concentrations, number of alginate molecules per unit volume and the number of binding sites for Ca 2+ cation also increased. As a result, formation of high, densely cross-linked gel structure is expected [17]. This would explain why the diameter and membrane thickness of the capsules formed with high alginate concentration are lower than those formed with low alginate concentrations.…”

Section: Influence Of Alginate Solution Formulationmentioning

confidence: 99%

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Alginate liquid core capsule formation using the simple extrusion dripping method

Lee

Ibrahim

Chu

et al. 2014

Journal of Polymer Engineering

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Liquid core capsules have been widely used in various biotechnological applications. The capsules could be formed by the simple extrusion dripping method. However, the method requires strict control of several process variables in order to form spherical capsules. The aim of this study was to systematically investigate the influence of process variables of the method on the capsule size and shape. The results showed that the capsules diameter was decreased when the concentration of alginate solution was increased. The capsule diameter was increased when the gelation time and dripping tip diameter were increased. The membrane thickness of the capsules was significantly increased by the concentration of calcium chloride, gelation time and dripping tip diameter. However, the concentration of alginate gave the opposite trend on the membrane thickness of the capsules. As a recommendation, uniform and spherical alginate liquid core capsules could be formed when concentration of calcium chloride was > 10 g/l, the concentration of alginate solution was > 5 g/l and < 20 g/l, gelation solution height in between 1.7 cm and 3.2 cm, and stirring rate of the gelation bath was in the range of 400-500 rpm.

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Section: Influence Of Liquid Core Solution Formulationsupporting

confidence: 93%

Section: Influence Of Alginate Solution Formulationsupporting

confidence: 92%

Section: Influence Of Gelation Timesupporting

confidence: 92%

Section: Influence Of Alginate Solution Formulationmentioning

confidence: 99%

See 2 more Smart Citations

Alginate liquid core capsule formation using the simple extrusion dripping method

Lee

Ibrahim

Chu

et al. 2014

Journal of Polymer Engineering

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“…Whole cell immobilization technique has several advantages over free cell systems such as higher yield of enzyme activity, higher operational stability, greater resistance to environmental perturbations, lower enzyme cost and repeated use of biocatalyst (Mamo and Gessesse 2000;Nampoothiri et al 2005;Konsoula and Liakopoulou 2006). The most common immobilization matrix used nowadays is alginate, since encapsulation in Ca-alginate gels occurs under very mild conditions and is characterized by the low cost (Bladino et al 2001;Dey et al 2003). …”

Section: Introductionmentioning

confidence: 99%