Immobilization of Glucose Oxidase on Modified-Carbon-Paste-Electrodes for Microfuel Cell

Ambarsari, Laksmi; Setyawati, Inda; Kurniasih, Rini; Kurniatin, Popi Asri; Maddu, Akhiruddin

doi:10.22146/ijc.21183

Cited by 3 publications

(6 citation statements)

References 17 publications

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“…9 In recent years, the glucose measurements are based on immobilization of GOx as complicated systems for detecting H2O2 concentration which is quick and accurate. [10][11][12] Ambarsari et al 13 reported a glucose biosensor utilizing nanofiber-polyaniline/glutaraldehyde as an immobilization matrix for GOx in an enzymatic fuel cell system. GOx has been isolated from a local fungus collection of Aspergillus niger (IPBCC.08.610) with yield and activity was 72.86% and 27.77 U/mg, respectively.…”

Section: Glucose + O → H O + Gluconic Acidmentioning

confidence: 99%

“…The modified carbon paste electrode (MCPE) was made with 2 mg nanofiber-polyaniline by thoroughly mixing in mortar 100 µL paraffin with 0.15 g of graphite powder according to method Ambarsari et al 13 with slight modification. For the preparation of MCPE capillary glass (diameter of 3 mm) the tubes were filled which carbon paste with the size of 3, 5, 7 and 10 mm.…”

Section: Preparation Of Modified Carbon Paste Electrodesmentioning

confidence: 99%

“…Immobilization of GOx into MCPE was done with glutaraldehyde as a crosslinker according to a previous method. 13,14 Briefly, the volume (of 5, 10, 15, and 20 µL) of GOx were mixed with 1 mg BSA, 50 µL of 0.1 M acetic buffer (pH 4.5) and 30 µL glutaraldehyde (of 0.5, 1.0, 1.5, 2.0, and 2.5%). The mixture was placed into micro tubes and then shake slowly.…”

Section: Preparation Of Glucose Oxidase/modified Carbon Paste Electrode (Gox/mcpe)mentioning

confidence: 99%

“…Maximum current was 5.39 mA (carbon paste size of 3x3 mm 2 ), which was better than that reported in our previous studies. 13,14…”

Section: Optimization Of Carbon Paste Size On Gox/mcpementioning

confidence: 99%

“…This result indicated that the variation of the GOx volume was useful in increasing the electroconductivity of GOx/MCPE, which was better than that reported in our previous studies. 13,14 Therefore, the volume of 5 µL of GOx was used in future studies.…”

Section: Optimization Of Gox Volume On Gox/mcpementioning

confidence: 99%

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"An Optimized Glucose Biosensor as a Potential Micro-Fuel Cell"

2018

RJC

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Glucose oxidase (GOx), a flavoprotein enzyme with the nomenclature of E.C.1.1.3.4, is widely employed in the different sectors such as an implantable fuel cell. In this paper, optimal conditions for the size of carbon paste, GOx volume, and glutaraldehyde concentrations for fabrication of polyaniline nanofiber modified carbon paste electrodes (GOx/MCPE) were investigated. The optimum parameters of the carbon paste size, GOx volume, and glutaraldehyde concentration were found to be 3 mm x 3 mm, 5 µL and 0.5%, respectively. The maximum current in carbon paste size (3 mm x 3 mm), GOx volume (5 µL) and glutaraldehyde concentration (0.5%) were estimated to be 5.39 mA, 4.12 mA, and 7.10 mA, respectively. This GOx/MCPE showed great potential in fuel cell application.

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Section: Glucose + O → H O + Gluconic Acidmentioning

confidence: 99%

Section: Preparation Of Modified Carbon Paste Electrodesmentioning

confidence: 99%

Section: Preparation Of Glucose Oxidase/modified Carbon Paste Electrode (Gox/mcpe)mentioning

confidence: 99%

“…Maximum current was 5.39 mA (carbon paste size of 3x3 mm 2 ), which was better than that reported in our previous studies. 13,14…”

Section: Optimization Of Carbon Paste Size On Gox/mcpementioning

confidence: 99%

Section: Optimization Of Gox Volume On Gox/mcpementioning

confidence: 99%

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"An Optimized Glucose Biosensor as a Potential Micro-Fuel Cell"

2018

RJC

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In Silico Analysis of Glucose Oxidase H516r and H516d Mutations for an Enzymatic Fuel Cell

Puspita

Ambarsari

Adiva

et al. 2021

Valensi

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Glucose oxidase (GOx) is an oxido-reductase enzyme that catalyzes glucose into hydrogen peroxide and glucono delta-lactone (GDL). GOx has the potential to be used in the medical field. Numerous research concerning the usage of GOx to create enzymatic biofuel cells have been done, nevertheless the results obtained have not been optimal. This research aims to increase the Km values of GOx in order to increase its potential as a material for an enzymatic fuel cell. The amino acid histidine in position 516 is a residue in the active site that plays an important part in the process of glucose oxidation. In this research we mutated H516 by in silico twice resulting in the mutants R516 and D516. The mutations resulted in a change of the docking area for both mutants and in the docking affinity for H516D resulting in higher Km values. This shows that the H516 residue plays an important part in the functions of glucose oxidase and mutation into aspartate could improve glucose oxidase based enzymatic fuel cells.

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Improvement Strategies, Cost Effective Production, and Potential Applications of Fungal Glucose Oxidase (GOD): Current Updates

et al. 2017

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Fungal glucose oxidase (GOD) is widely employed in the different sectors of food industries for use in baking products, dry egg powder, beverages, and gluconic acid production. GOD also has several other novel applications in chemical, pharmaceutical, textile, and other biotechnological industries. The electrochemical suitability of GOD catalyzed reactions has enabled its successful use in bioelectronic devices, particularly biofuel cells, and biosensors. Other crucial aspects of GOD such as improved feeding efficiency in response to GOD supplemental diet, roles in antimicrobial activities, and enhancing pathogen defense response, thereby providing induced resistance in plants have also been reported. Moreover, the medical science, another emerging branch where GOD was recently reported to induce several apoptosis characteristics as well as cellular senescence by downregulating Klotho gene expression. These widespread applications of GOD have led to increased demand for more extensive research to improve its production, characterization, and enhanced stability to enable long term usages. Currently, GOD is mainly produced and purified from Aspergillus niger and Penicillium species, but the yield is relatively low and the purification process is troublesome. It is practical to build an excellent GOD-producing strain. Therefore, the present review describes innovative methods of enhancing fungal GOD production by using genetic and non-genetic approaches in-depth along with purification techniques. The review also highlights current research progress in the cost effective production of GOD, including key advances, potential applications and limitations. Therefore, there is an extensive need to commercialize these processes by developing and optimizing novel strategies for cost effective GOD production.

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Immobilization of Glucose Oxidase on Modified-Carbon-Paste-Electrodes for Microfuel Cell

Abstract: ABSTRACT).

Cited by 3 publications

References 17 publications

"An Optimized Glucose Biosensor as a Potential Micro-Fuel Cell"

"An Optimized Glucose Biosensor as a Potential Micro-Fuel Cell"

In Silico Analysis of Glucose Oxidase H516r and H516d Mutations for an Enzymatic Fuel Cell

Improvement Strategies, Cost Effective Production, and Potential Applications of Fungal Glucose Oxidase (GOD): Current Updates

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