Immobilization of glucose oxidase on gold nanoparticles modified Au electrode for the construction of biosensor

Zhang, Suxia; Wang, Nü; Niu, Yaming; Sun, Chang Q.

doi:10.1016/j.snb.2005.01.003

Cited by 173 publications

(75 citation statements)

References 22 publications

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“…Their bimolecular rate constants for FADH 2 -GOx oxidation range from 3 × 10 4 to 8 × 10 6 M -1 s -1 . 148 The most intensively studied ferrocene-derivatives are ferrocenemethanol 88,90,91,[149][150][151][152][153][154][155][156][157][158][159][160][161] and ferrocenecarboxylic acid. 155,[162][163][164] Several of the ferrocenes meet the requirements for application in blood-glucose analyzers, which include high solubility in water, fast electron-shuttling, stability, and pH-independence of the redox potential.…”

Section: Metal-organic Mediatorsmentioning

confidence: 99%

Electrochemical Glucose Sensors and Their Applications in Diabetes Management

2008

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About 6,000 peer reviewed articles have been published on electrochemical glucose assays and sensors, of which 700 were published in the 2005-2006 two-year period. Their number makes a full review of the literature, or even of the most recent advances, impossible. Nevertheless, this review should acquaint the reader with the fundamentals of the electrochemistry of glucose and provide a perspective of the evolution of the electrochemical glucose assays and monitors helping diabetic people, who constitute about 5% of the world's population. Because of the large number of diabetic people, no assay is performed more frequently than that of glucose. Most of these assays are electrochemical. The reader interested also in nonelectrochemical assays used in, or proposed for, the management of diabetes is referred to a 2007 review of Kondepati and Heise. 1 Adam Heller was born in 1933. Surviving the Holocaust, he arrived in Israel in 1945. He received his M.Sc. in Chemistry and Physics in 1957, then his PhD in Organic Chemistry in 1961 from Ernst David Bergman at the Hebrew University in Jerusalem. He postdoced at UC Berkeley (1962-3) and at Bell Laboratories . At GTE Labs (1964-1975 he built the first Nd 3+ liquid laser and, with J. J. Auborn, the still worldwide used Li/SOCl 2 battery. At Bell Labs (1975)(1976)(1977)(1978)(1979)(1980)(1981)(1982)(1983)(1984)(1985)(1986)(1987)(1988) he designed the first >10% efficient electrochemical solar cells and the first >10% efficient hydrogengenerating solar-powered photoelectrode. He also headed Bell Labs' Electronic Materials Research Department (1977)(1978)(1979)(1980)(1981)(1982)(1983)(1984)(1985)(1986)(1987)(1988), which developed part of the high density chip interconnection technology underlying the miniaturization of portable electronic devices. He was appointed to the Ernest Cockrell Sr. Chair in Engineering of the University of Texas at Austin in 1988, and in 2001 became one of UT's first Research Professors. At UT he pioneered the electrical wiring of enzymes. In 1996 he cofounded with his son Ephraim Heller TheraSense Inc., now part of Abbott Diabetes Care, to improve the lives of diabetic people. The company introduced in 2000 the blood sugar monitor FreeStyle, a thin-layer microcoulometer utilizing only 300 nL of blood, so little that it was, for the first time, painlessly obtained. In 2007 it provided for more than 1 billion painless glucose assays. After alleviating the pain of diabetes monitoring, FreeStyle Navigator, based on the electrical wiring of glucose oxidase, introduced in 2007 in Europe and Israel and in 2008 in the U.S., removed the worry of diabetic people by continuously monitoring their glucose levels. Heller aims his work at alleviating suffering through bioelectrochemistry.

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Section: Metal-organic Mediatorsmentioning

confidence: 99%

Electrochemical Glucose Sensors and Their Applications in Diabetes Management

2008

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“…101 The construction of a glucose amperometric biosensor by using this kind of modification can be made by subsequent immersion of silica modified Au electrode into different solutions: AuNPs, cysteamine and finally GOx. 102 In a similar assembly, HRP was covalently attached to AuNPs immobilized on a silica network. 103 Recently it was reported an interesting work involving the assembly of AuNPs and GOx in a tridimensional silica network by means of chemioluminescent reaction between the H 2 O 2 produced in enzymatic reaction and luminol catalyzed by AuNPs.…”

Section: Redox Protein Biosensors Designed With Aunpsmentioning

confidence: 99%

Biosensors based on gold nanostructures

Vidotti¹,

Carvalhal²,

Ferreira³

et al. 2011

J. Braz. Chem. Soc.

130

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O presente trabalho de revisão aborda os mais recentes avanços na tecnologia de biossensores alcançados através da montagem de biomoléculas associada com nanopartículas de ouro na construção de dispositivos analíticos. Esta revisão está dividida de acordo com a biomolécula empregada no desenvolvimento de biossensores: (i) compostos imunológicos; (ii) DNA/RNA funcionais; e (iii) enzimas e proteínas Heme. A fim de facilitar a compreensão, cada seção foi subdividida de acordo com o modo de transdução. Os imunossensores contendo nanopartículas de ouro têm uma ampla gama de aplicações nos campos alimentício, ambiental, farmacêutico, químico e de diagnósticos clínicos. As nanopartículas foram empregadas para melhorar o sinal analítico ou a imobilização dos imunocompostos. Em outra seção, os biossensores DNA/ RNA empregando nanoestruturas de ouro como labels e biossensores label-free associados a nanoestruturas de ouro como transdutores foram sistematicamente relatados para a rápida identificação de patógenos, espécies de interesse ambiental e diagnóstico clínico. A inclusão de nanopartículas de ouro em eletrodos modificados aumenta a transferência de elétrons entre o transdutor e a biomolécula proporcionando um melhor desempenho quando enzimas e proteínas redox heme são usados. Biossensores para a detecção e quantificação de glicose e peróxido de hidrogênio foram também discutidos.The present review discusses the latest advances in biosensor technology achieved by the assembly of biomolecules associated with gold nanoparticles in analytical devices. This review is divided in sections according to the biomolecule employed in the biosensor development: (i) immunocompounds; (ii) DNA/RNA and functional DNA/RNA; and (iii) enzymes and Heme proteins. In order to facilitate the comprehension each section was subdivided according to the transduction mode. Gold nanoparticles based immunosensors have a wide range of applications in food, environmental, pharmaceutical, chemistry and clinical diagnostics. The nanoparticles were employed to improve whether the analytical signal or the immunocompounds immobilization. In another section, biosensors based on DNA/RNA biomolecules employing gold nanostructures as labels and label-free funtional DNA/RNA biosensors associated to gold nanostructures as tranducers were systematically reported for rapid identification of pathogens, species of environmental interest and clinical diagnostics, respectively. The inclusion of gold nanoparticles in modified electrodes itself enhances the electron transfer between the transducer and biomolecules leading to improved bioanalytical devices when redox enzymes and heme proteins are used. Biosensors for the detection and quantification of glucose and hydrogen peroxide are discussed as well.

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“…Another strategy to measure at lower potential consists to use other mediators as Prussian blue [7,8], Meldola blue [9], ferrocene [10,11] or ferrocyanide [12,13]. Nanomaterials as gold nanoparticles [14,15], carbon nanotubes [16] have also been used in order to improve the electronic transfer between the electroactive center of the enzyme and the surface electrode (3 rd generation sensor) [17]. These sensors would not need any mediator but in the most of the cases, those sensors with nanogolds or nanotubes need some compound as mediator [18,19], which can offer an improvement of sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Enzymatic Sensor Using Mediator‐Screen‐Printed Carbon Electrodes

et al. 2010

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A new glucose sensor was developed using screen-printed ferrocyanide/carbon electrodes. The ferrocyanide is included in the carbon ink of the commercial screen-printed electrode. The immobilization of enzymes glucose oxidase (Gox) and horseradish peroxidase (HRP) were carried out in a very easy way. An aliquot of 10 mL of a Gox/ HRP mixture was deposited on the electrode surface and left there until dried (approximately 1 h) at room temperature. The ferricyanide generated enzymatically was detected amperometrically applying a potential of À0.1 V (vs. Ag pseudo reference electrode). The sensor, so constructed, shows a good sensitivity to glucose (À2.12 mA/mM) with a slope deviation of AE 0.06 mA/mM and a linear range comprised between 0.05 and 1 mM of glucose, with a limit of detection of 0.025 mM. These sensors show good intersensor reproducibility and a high stability. When they are stored at 4 8C, no significant changes in the slope value of the glucose calibration plot were found after 3 months. Glucose was determined in real samples as honey, blood, drink for babies and glucosed drink with a great accuracy.

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Immobilization of glucose oxidase on gold nanoparticles modified Au electrode for the construction of biosensor

Cited by 173 publications

References 22 publications

Electrochemical Glucose Sensors and Their Applications in Diabetes Management

Electrochemical Glucose Sensors and Their Applications in Diabetes Management

Biosensors based on gold nanostructures

Enzymatic Sensor Using Mediator‐Screen‐Printed Carbon Electrodes

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