Immobilization of glucose oxidase on electrodeposited nickel oxide nanoparticles: Direct electron transfer and electrocatalytic activity

Salimi, Abdollah; Sharifi, Ensiyeh; Noorbakhsh, Abdollah; Soltanian, Saeid

doi:10.1016/j.bios.2007.02.002

Cited by 228 publications

(131 citation statements)

References 60 publications

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“…3͑b͔͒ of the fabricated biosensor increases linearly ͑50-400 mg/ dL͒ with increasing glucose concentration with correlation coefficient ͑R͒ as 0.987 indicating good electrocatalytic behavior of GOx / CeO 2 / Au bioelectrode. Compared to similar glucose biosensors, [8][9][10] this sensor shows higher sensitivity ͑0.00287 A / mg dL −1 cm −2 ͒ and low detection limit ͑12.0 M͒.…”

Section: -mentioning

confidence: 96%

“…8,9 The high GOx affinity to glucose is assigned to biocompatibility, large surface area, and high electron communication capability of CeO 2 nanoparticles in nanostructured GOx / CeO 2 / Au electrode surface. This glucose sensor reaches more than 95% steady state current in less than 5 s indicating faster electron communication between GOx and nanostructured CeO 2 electrode.…”

Section: -mentioning

confidence: 99%

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Sol-gel derived nanostructured cerium oxide film for glucose sensor

Ansari

Solanki

Malhotra

2008

Applied Physics Letters

143

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Sol-gel derived nanostructured cerium oxide ͑CeO 2 ͒ film deposited on gold ͑Au͒ electrode has been utilized for physisorption of glucose oxidase ͑GOx͒. X-ray diffraction, atomic force microscopy, UV-visible spectroscopy, and electrochemical techniques have been used to characterize sol-gel derived CeO 2 / Au electrode and GOx / CeO 2 / Au bioelectrode. The response characteristics of the glucose bioelectrode ͑GOx / CeO 2 / Au͒ indicate linearity, detection limit and shelf-life as 50-400 mg/ dL, 12.0 M, and 12 weeks, respectively. The value of apparent Michaelis-Menten constant ͑K m ͒ of GOx / CeO 2 / Au bioelectrode has been found to be 13.55 M.

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Section: -mentioning

confidence: 96%

Section: -mentioning

confidence: 99%

Sol-gel derived nanostructured cerium oxide film for glucose sensor

Ansari

Solanki

Malhotra

2008

Applied Physics Letters

143

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“…What is more, NiO nanoparticles play an accelerating the catalytic role in the redox reaction system due to the formation of some suspension unsaturated bonds on the surface of nanoparticle. 19,20 With regard to the electrocatalytic activity of the modified electrode to H 2 O 2 , the steady-state amperometry technique is a classical electrochemical method. The steady-state current reflected the electrocatalytic ability of the HRP/Nano-NiO/MWCNTs/PANI-modified electrode at a suitable applying potential.…”

Section: Resultsmentioning

confidence: 99%

Nickel Oxide Nanoparticles Immobilized on MWCNTs/PANI Composite Film as an Electron Transfer Facilitator for Horseradish Peroxidase: Direct Electron Transfer and H₂O₂Determination

Zhang

Yang

Guo

et al. 2016

J. Electrochem. Soc.

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In this paper, we developed a new method for immobilization of horseradish peroxidase (HRP) on Nano-NiO/ MWCNTs/PANI composite film, which can effectively promote the direct electron transfer and electrocatalysis of enzyme in neutral solution. This composite film modified electrode was systematically investigated by altering the thickness of composite film, pH value of solution, and the mass ratio of MWCNT to NiO nanoparticles. The study showed that the effective immobilization of horseradish peroxidase on Nano-NiO/ MWCNTs/PANI was 30 cycles of cyclic voltammetry for the preparation of PANI film and the mass ratio of 10:1. The composite film possesses a high catalytic activity in pH 6.8 buffer solution containing H 2 O 2 . The plot of the response current to H 2 O 2 concentration exhibited a linear relation at a range of 3.0 × 10 −6 to 4.3 × 10 −4 M, with a correlation coefficient of 0.998. The detection limit is 4.3 × 10 −7 M(S/N = 3). These results indicated that the modified electrode was beneficial to retain the catalytic activity of the enzyme, and possessed good stability and reproducibility. The electrochemical technology has been most extensively applied in the determination of H 2 O 2 as an inexpensive and effective way. However, it is difficult to carry out slow reaction kinetics for many electrode materials in detecting hydrogen peroxide process, and is required a high overpotential to avoid interference of other compounds in the same potential region. Hence, the electrode was generally modified by polymer film to rapidly and sensitively measure H 2 O 2 . In the past research, polyaniline (PANI) has been widely investigated as a key modifying material in chemical and biological sensors 1-3 due to good environmental stability, facile synthesis, electrical and optical properties, and unique doping/de-doping behavior, 4,5 and its electrical property can be reversibly modulated by changing the state of oxidation. Additionally, it is suitable for PANI film due to a moderating effect for enzyme catalytic reaction signal and compatibility. 6,7 However, the conductivity of PANI depends on the pH values of solutions. Generally, the excellent redox characteristics are exhibited in acidic aqueous solutions at pH up to about 5, while a low conductivity can be found in a neutral solution and/or alkaline solutions. Therefore, the modified electrode with polyaniline cannot fabricate a high-sensitivity biosensor. Moreover, the low conductivity cannot effectively mediate electron transfer between redox enzymes and conductive supports in biosensor systems. To improve the conductivity of polyaniline film, a conductive material with high conductivity, such as carbon nanotube(CNT), is introduced into PANI matrix to form conducting polymer/carbon nanotube composites. 8,9 The addition of CNT promotes the electron transfer rate between film and electrode in this composite, and possesses high electroactivity in a neutral pH environment. [10][11][12] The combination between CNTs and polymer matrix could effectively improve sol...

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“…It was found that CNTs can promote the electroactive surface area and can accelerate the rate of electron transfer between the redoxactive enzyme and the electrode. A novel approach have introduced for glucose sensing by Salimi et al, (2007). They found the excellent electrochemical performance of electrochemically co-deposited nickel oxide nanoparticles and GOx onto the glassy carbon electrode surface.…”

Section: Glucose Biosensorsmentioning

confidence: 99%

Nanostructured Metal Oxides Based Enzymatic Electrochemical Biosensors

Anees¹,

Alhoshan²,

AlSalhi³

et al. 2010

Biosensors

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Immobilization of glucose oxidase on electrodeposited nickel oxide nanoparticles: Direct electron transfer and electrocatalytic activity

Cited by 228 publications

References 60 publications

Sol-gel derived nanostructured cerium oxide film for glucose sensor

Sol-gel derived nanostructured cerium oxide film for glucose sensor

Nickel Oxide Nanoparticles Immobilized on MWCNTs/PANI Composite Film as an Electron Transfer Facilitator for Horseradish Peroxidase: Direct Electron Transfer and H₂O₂Determination

Nanostructured Metal Oxides Based Enzymatic Electrochemical Biosensors

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Immobilization of glucose oxidase on electrodeposited nickel oxide nanoparticles: Direct electron transfer and electrocatalytic activity

Cited by 228 publications

References 60 publications

Sol-gel derived nanostructured cerium oxide film for glucose sensor

Sol-gel derived nanostructured cerium oxide film for glucose sensor

Nickel Oxide Nanoparticles Immobilized on MWCNTs/PANI Composite Film as an Electron Transfer Facilitator for Horseradish Peroxidase: Direct Electron Transfer and H2O2Determination

Nanostructured Metal Oxides Based Enzymatic Electrochemical Biosensors

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Nickel Oxide Nanoparticles Immobilized on MWCNTs/PANI Composite Film as an Electron Transfer Facilitator for Horseradish Peroxidase: Direct Electron Transfer and H₂O₂Determination