Immobilization of glucose oxidase into polyaniline nanofiber matrix for biofuel cell applications

Encapsulation and immobilization of papain in electrospun nanofibrous membranes of PVA cross-linked with glutaraldehyde vapor

“…Other uses of enzyme immobilization by electrospun nanofibers include biofuel cell applications, ethanol production, removal of pollutants from contaminated soil, sugar production from microalgae, antibacterial applications, etc. [25,26,[30][31][32]. In this work, we report the immobilization of the enzyme papain onto electrospun poly(vinyl alcohol) (PVA) nanofibers.…”

Cross-linked glucose oxidase clusters for biofuel cell anode catalysts

“…Given a predicted volume (protein only) of the GOx dimer as 168.17 nm 3 , each CEC would contain approximately 3.71 × 10 9 GOx dimers. It is conceivable that while the size of the CECs is substantial in comparison to that of the CNTs, an increased number of smaller CECs more uniformly distributed along CNT surface, similar to other studies of GOx localization onto electrode surfaces [16, 29, 30, 51-53], would be more effective for DET. The larger CECs observed may be a result of a lack of accessibility to individual CNTs by the protein either during the initial incubation with the GOx protein or due to the spontaneous clumping behaviour of the protein with itself upon ammonium sulfate precipitation.…”

Preparing Co3O4 urchin-like hollow microspheres self-supporting architecture for improved glucose biosensing performance

“…The cofactor is then reoxidized, causing electrons transfer and yielding hydrogen peroxide. The formation of H 2 O 2 can subsequently be detected to monitor the concentration of glucose [16,17]. Consequently, the key points of the biosensor could be elaborated from two aspects, one is the enzyme catalysis which is related to the oxidation of glucose, the other one is the material catalysis associated with electrochemical oxidation of the H 2 O 2 at the detection potential.…”