Immobilization of ?-galactosidase on nylon membranes grafted with diethylenglycol dimethacrylate (DGDA) by ?-radiation: Effect of membrane pore size

Abstract

The dependence of grafting and catalytic activity on the pore size of nylon membranes grafted with different DGDA concentrations and loaded with β‐galactosidase is discussed. Membrane pore sizes were 0.2, 1.2, and 3.0 μm. Grafting and enzyme activity, all other experimental conditions being the same, decrease with the increase of membrane pore size. The membranes have been characterized also with reference to pH and temperature. The experimental conditions giving the best performance for each of the three membranes have been identified. In all cases the membranes giving the best results are those having 0.2 μm pore size. The advantages of using in non‐isothermal bioreactors a single membrane, catalytic and hydrophobic at the same time, are also indicated. © 1999 John Wiley & Sons, Inc. Adv Polym Techn 18: 109–123, 1999

Photopolymerizable Materials in Biosensorics

“…1993;Doretti et al, 1994;Jimenez et al, 1995;Macca et al, 1995;Moser et al, 1995;Gooding & Hall, 1996;Lesho & Sheppard, 1996;Ambrose & Meyerhoff, 1997;Wróblewski et al, 1997;Doretti et al, 1998;Hall et al, 1999;Kolytcheva et al, 1999;Mohy et al, 1999;Rehman et al, 1999). 2-(hydroxyethyl)methacrylate (HEMA) is used the mostet often.…”