Immobilization of feruloyl esterases in mesoporous materials leads to improved transesterification yield

Thörn, Christian; Gustafsson, Hanna; Olsson, Lisbeth

doi:10.1016/j.molcatb.2011.05.002

Cited by 55 publications

(42 citation statements)

References 40 publications

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“…Silicas of different dispersive-morphological parameters and porous structures have been proposed. A representative silica used for the enzyme immobilization on a large scale is mesoporous silica SBA-15 (Santa Barbara Amorphous) with hexagonal array of pores (Salis et al 2010;Thorn et al 2011). It is characterized by small pores, from 5 to about 30 nm in diameter and a hexagonal array of pores (Grudzień et al, 2006;2007;Hartmann and Kostrov 2013).…”

Section: Inorganic Carriersmentioning

confidence: 99%

Enzyme immobilization by adsorption: a review

2014

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Endowed with unparalleled high catalytic activity and selectivity, enzymes offer enormous potential as catalysts in practical applications. These applications, however, are seriously hampered by enzymes' low thermal and chemical stabilities. One way to improve these stabilities is the enzyme immobilization. Among various tested methods of this process that make use of different enzyme-carrier interactions, immobilization by adsorption on solid carriers has appeared most common. According to these findings, in this review we present a comparative analysis of the literature reports on the recent trends in the immobilization of the enzymes by adsorption. This thorough study was prepared in order to provide a deeper understanding of the process. Both carriers, carrier modifiers and procedures developed for effective adsorption of the enzymes are discussed. The review may thus be helpful in choosing the right adsorption scheme for a given enzyme to achieve the improvement of its stability and activity for a specific application.

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Section: Inorganic Carriersmentioning

confidence: 99%

Enzyme immobilization by adsorption: a review

2014

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“…For instance, lipase immobilized on non-porous particles has been reported to have a much lower specific activity than lipase immobilized in mesoporous particles [20]. In the case of silica, non-porous or solid particles exist in a broad range of particles sizes comparable to the dimensions of mesoporous particles [18,20,[91][92][93].…”

Section: Comparison With Non-porous Particlesmentioning

confidence: 99%

Enzymes immobilized in mesoporous silica: A physical–chemical perspective

Carlsson

Gustafsson

Thörn

et al. 2014

Advances in Colloid and Interface Science

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211

167

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“…The goal of many of these studies was to create more hydrophobic feruloylated compounds such as butyl ferulate (Vafiadi et al 2008;Thörn et al 2011) and feruloylated monoacyl-and diacyl-glycerol (Sun et al 2007b(Sun et al , 2009). On the other hand, Topakas et al (2005) succeeded in synthesizing feruloylated L-arabinose with Sporotrichum thermophile FAE (StFaeC), which is the first demonstration of enzymatic feruloylation of hydrophilic compounds.…”

Section: Discussionmentioning

confidence: 99%

“…Esterification is one way of modifying the physical properties of FA. Many feruloylated compounds are synthesized by lipases or FAEs to increase their hydrophobicity (Vafiadi et al 2008;Thörn et al 2011;Sun et al 2007bSun et al , 2009). On the other hand, little has been done to synthesize feruloylated compounds with high solubility in water.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of highly water-soluble feruloyl diglycerols by esterification of an Aspergillus niger feruloyl esterase

Kikugawa

Tsuchiyama

Kai

et al. 2012

Appl Microbiol Biotechnol

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Ferulic acid (FA) is a component of plant cell walls that has applications in food, cosmetic, and health products, but its applications are limited by its high insolubility. We synthesized water-soluble FA derivatives by esterification of FA with diglycerol (DG) using feruloyl esterase purified from a commercial enzyme preparation produced by Aspergillus niger. The major reaction product, FA-DG1, was determined to be γ-feruloyl-α,α'-DG by NMR and electrospray ionization mass spectrometry analyses. FA-DG1 is a sticky liquid whose water solubility (>980 mg/ml) is dramatically higher than that of FA (0.69 mg/ml). Suitable conditions for esterification of FA with DG were 100 mg of FA in the presence of 1 g of DG and 0.1 ml of 1 M phosphate buffer (pH 6.0) at 50 °C under reduced pressure. Under these conditions, 168 mg of feruloyl DGs (FA-DG1, 2, and 3) was obtained, corresponding to a 95 % conversion rate of FA. We also developed a batch method which resulted in synthesis of 729 mg of feruloyl DGs and 168 mg of diferuloyl DGs from 600 mg of FA and 1 g of DG (corresponding to conversion of 69 % of the FA to feruloyl DGs and 21 % of the FA to diferuloyl DGs). As an anti-oxidant, feruloyl DGs were essentially equal to FA and butyl hydroxytoluene in scavenging 1,1-diphenyl-2-picrylhydrazyl radicals. In contrast, the scavenging abilities of diferuloyl DGs were twice those of feruloyl DGs.

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Immobilization of feruloyl esterases in mesoporous materials leads to improved transesterification yield

Cited by 55 publications

References 40 publications

Enzyme immobilization by adsorption: a review

Enzyme immobilization by adsorption: a review

Enzymes immobilized in mesoporous silica: A physical–chemical perspective

Synthesis of highly water-soluble feruloyl diglycerols by esterification of an Aspergillus niger feruloyl esterase

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