Immobilization of enzymes to porous‐bead polymers and silica gels activated by graft polymerization of 2,3‐epoxypropyl methacrylate

Abstract

Three types of organic polymers and bead‐shape silica gels were activated by graft polymerization of 2,3‐epoxypropyl methacrylate; in some cases, epoxide groups on the support surface were modified to NH2 groups. Eight active matrices so obtained were assessed as supports for immobilized enzymes using peroxidase, glucoamylase and urease. The immobilization yield of protein and specific activities of enzymes were better with supports containing NH2 groups than with those containing epoxide spacer arms. Maximum enzyme immobilization and storage stabilities were obtained with silica‐gel beads activated by graft polymerization of 2,3‐epoxypropyl methacrylate. With all eight matrices tested, the immobilized enzymes showed good stability with not less than 82% of the original activity persisting after 28 days. The developed matrices have potential for use in process‐scale biotechnological operations.

Spring cabbage peroxidases – Potential tool in biocatalysis and bioelectrocatalysis

“…It is known that this heterogeneous heme protein presents quite significant heat stability, optimum activity at neutral (6-8) pH values and is suitable for effective immobilization (Chmielnicka, 1966;Robinson, 1981,1987;Wó jcik et al, 1990). These properties make cabbage peroxidases highly promising for biotechnological applications, specially for constructions of stable and highly effective bioelectrocatalytic systems for oxygen reduction, with respect to e.g.…”

Cinnamic ester of D‐sorbitol for immobilization of mushroom tyrosinase

“…Attempts have been made to use immobilized tyrosinase for the commercial production of L ‐dopa,15, 16 for the removal of phenolic compounds from wastewater,17, 18 as biosensors to detect pollutants in environmental samples,19, 20 for the electrochemical determination of hydrogen peroxide,12 or for the analysis of thiol‐containing compounds 21. It is widely accepted that more attention should be paid to the effect of the polymeric supports used for immobilization on the catalytic activity22–24 and stability10, 12, 25–27 of immobilized enzymes. In this study the polymeric support used for tyrosinase immobilization was the totally cinnamoylated derivative of D ‐sorbitol (SOTCN) on glass beads.…”